sirvan nasimi; M. Sharafi; abdolhossein shahverdi
Abstract
Serotonergic axis has an important role in reproductive process of breeder rooster flocks. The aim of this study was to evaluate semen quality parameters of Ross old broiler breeder roosters, which received serotonin inhibitor (PCPA). In this study, 16 Ross broiler breeder rooster assigned into four ...
Read More
Serotonergic axis has an important role in reproductive process of breeder rooster flocks. The aim of this study was to evaluate semen quality parameters of Ross old broiler breeder roosters, which received serotonin inhibitor (PCPA). In this study, 16 Ross broiler breeder rooster assigned into four groups and received 0, 20, 40 and 80 ppm PAPA during 4 weeks. Then semen samples were collected and frozen. After freezing-thawing process, motion parameters, viability, membrane integrity, morphology, acrosome integrity, mitochondria activity, lipid peroxidation and DNA fragmentation were evaluated. In results, using 20 and 40 ppm PCPA improved total motility, progressive motility, membrane integrity, acrosome integrity, viability and decreased apoptosis rate in frozen-thawed semen samples. Using PCPA did not have any significant effect on morphology, mitochondria activity, lipid peroxidation and DNA fragmentation of semen samples. It seems to using 20 and 40 ppm PCPA could be a suitable method to improve Ross old broiler breeder rooster semen quality parameters after freezing-thawing process.
seyed kamal hashemian; M. Mehri
Abstract
The objective of the present study was to investigate the preservative effect of green tea extract as a natural antioxidant on sperm quality parameters of rooster in the duration of an entire spermatogenesis cycle. Sixteen rooster divided into four aliquots and consumed diets supplemented with 0, 30, ...
Read More
The objective of the present study was to investigate the preservative effect of green tea extract as a natural antioxidant on sperm quality parameters of rooster in the duration of an entire spermatogenesis cycle. Sixteen rooster divided into four aliquots and consumed diets supplemented with 0, 30, 60, 90 mg of green tea extract/kg of feed. On 0, 14, 28, 42, 56, 70 days after start semen fluid were collected and CASA for sperm motility, progressive motility, linearity (LIN), curvilinear velocity (VCL), amplitude of lateral head displacement (ALH), average path velocity (VAP), straight line velocity (VSL), nonapoptotic sperm and DNA fragmentation (by staining sperm with acridine orange). Treatments 3 and 4 reduced apoptotic sperm and improved some of sperm motion parameters such as ALH (P<0.05). However, in last days of experiment, treatment 4 had adverse effects on VAP and VSL (P<0.05). The least amount of green tea extract (30 mg/kg of feed) improved VAP (P<0.05). The results showed that daily consumption of 60 mg green tea extract/kg of feed, as a natural antioxidant, could possibly lead to improve some of sperm motion parameters and reduced apoptosis.
