Document Type : Research Paper

Authors

Abstract

Totally, 3200 and 320 young worker bees were investigated morphologically and molecularly respectively. The four important honey bee microsatellite loci, A24, A28, A29, A113, indicated a considerable level of genetic diversity among the nation’s northwest honey bee populations and, considering average number of alleles per locus (3.25-5.75) and, average expected heterozygosity (0.445-0.701) high level of variability was shown. Moreover, according to molecular analysis of variance (AMOVA), intra-population and inter-population genetic diversity were estimated to be 70% and 30% respectively. In addition, statistical analysis of morphological characteristics using Principle Component Analysis (PCA) revealed that northwestern honey bee population of Iran could be divided into, at least, three groups. Considering the high genetical diversity of honey bee in northwest Iran, establishing queen rearing stations and executing interbreeding programs for honey bee population in northwest Iran seems to be necessary. Furthermore, considering the high level of polymorphism in the studied markers, they are recommended to be applied for Quantitative Trait Loci (QTL) mapping, Marker Assisted Selection (MAS) and, other related investigations.

1-    اسدی، ن. ا.، خدرزاده، ص. و امیری­ نیا، س. 1387؛بررسی تعادل هاردی- وینبرگ و چندشکلی در جمعیت­های زنبور عسل شمال­غرب ایران با استفاده از آنالیز ریزماهواره. مجله دانش و پژوهش علوم دامی. شماره 2: 1- 6.
2-       حسین پور، س.، دولتی، ل. ع.، ملایی، م. و سپهری، ر. 1391؛ بررسی کارآیی چهار روش مختلف استخراج DNA از زنبور عسل. خلاصه مقالات سومین همایش ملی بیوتکنولوژی کشاورزی ایران، 25 و 26 شهریور ماه 1391. مشهد. دانشگاه فردوسی مشهد.
3-       حلفی مسبوبی، ز.، روشنفکر، ه.­ ا.، تقی­بیگی نصیری، م. و بوجارپور، م.   1391؛ بررسی تنوع ژنتیکی زنبور عسل خوزستان با استفاده از نشانگرهای ریزماهواره­ای. خلاصه مقالات سومین همایش ملی بیوتکنولوژی کشاورزی ایران، 25 و 26 شهریور ماه 1391. مشهد. دانشگاه فردوسی مشهد.
4-       خدرزاده، ص. 1386؛ بررسی تنوع ژنتیکی و روابط فیلوژنتیکی جمعیت‌های زنبور عسل غرب ایران با استفاده از نشانگرهای ریزماهواره. پایان‌نامه کارشناسی‌ارشد علوم‌دامی، دانشکده کشاورزی و منابع طبیعی رامین.
 
5-    دفتر آمار و فناوری اطلاعات وزارت جهاد کشاورزی، 1389؛ آمارنامه کشاورزی، جلد دوم.
6-       طهماسبی، غ. ح. 1375؛ بررسی مرفولوژیکی و بیوشیمیایی توده های زنبور عسل ایران. پایان نامه دکترای حشره­شناسی کشاورزی، دانشگاه تربیت مدرس، دانشکده کشاورزی.
7- Bourgeois, L., Sylvester, A., Danka, R. and Rinderer, T., 2008; Comparison of Italian microsatellite DNA diversity among commercial queen breeder stocks honey bees in the United States and Italy. Journal of Apicultural Research and Bee World, 47: 93-98.
8- De La Rua, P., Galian, J., Serrano, J. and Moritz, R. F. A., 2001; Genetic structure and distinctiveness of Apis mellifera L. populations from the Canary Islands. Molecular Ecology,10: 1733–1742.
9- De La Rua, P., Galian, J., Serrano, J. and Moritz, R. F. A., 2003; Genetic structure of Balearic honeybee populations based on microsatellite polymorphism. Genetic Selection Evolution, 35: 339–350.
10- Doyle, J. J. and Doyle, L. L., 1987; A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemistry, 19: 11-15.
11- Estoup, A., Garnery, L., Solignac, M. and Cornuet, G. M., 1995; Microsatellite variation in honey bee (Apis mellifera L.) populations: hierarchical genetic structure and test of the infinite allele and stepwise mutation models. Genetics,140: 679-695.
12- Farhoud, H. J. and Kence, M., 2005; Morphometric and mtDNA analysis in honeybee populations (Apis mellifera L.) of north and northwest Iran. Proceedings of the Balkan scientific conference of biology in Plovdiv (Bulgaria) from 19th till 21st of May 2005, 2005, P: 594- 597.
13- Farshineh Adl, M. B., Gencer, H. V., Firatli, C and Bahreini, R., 2007; Morphometric characterization of Iranian (Apis mellifera meda), Central Anatolian (Apis mellifera anatoliaca) and Caucasian (Apis mellifera caucasica) honey bee populations. Journal of Apicultural Research and Bee World,46: 225-231.
14- Franck, P., Garnery, L., Loiseau, A., Oldroyd, B. P., Hepburn, H. R., Solignac, M., et al., 2001; Genetic diversity of the honeybee in Africa: microsatellite and mitochondrial data. Heredity,86: 420-430.
15- Garnery, L., Franck, P., Baudry, E., Vautrin, D., Cornuet, J. M. and Solignac, M., 1998; Genetic diversity of the west European honey bee (Apis mellifera mellifera and Apis mellifera iberica). II microsatellite loci. Genetic Selection Evolution, 30: 49-79.
16- Kandemir, I., Ozkan, A. and Moradi, M., 2004; A scientific note on allozyme variability in Persian honey bees (Apis mellifera meda) from the Elburz mountains in Iran. Apidologie, 35: 521–522
17-  Kence, M., Farhoud, H. J. and Tunca, R. I., 2009; Remove from marked records  morphometric and genetic variability of honey bee (Apis mellifera L.) populations from northern Iran. Journal of Apicultural Research, 48: 247-255.
18- Moradi, M. and Kandemir, I., 2004; Morphometric and allozyme variability in Persian bee population from the Alburz mountains, Iran. Iranian International  5: 151-166.
19-  Nei, M., 1978; Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics,89: 583–590.
20- Peakall, R. and Smouse, P. E., 2006; GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology,6: 288-295.
21- Royan, M., Rahimi, G., Esmaeilkhanian, S. and Ansari, Z., 2007; A study on the genetic diversity of the Apis mellifera meda population in the south coast of the Caspian Sea using microsatellite markers. Journal of Apicultural Research and Bee World,46: 236-241.
22- Ruttner, F., 1988; Biogeography and taxonomy of honeybees. Berlin: Springer-Verlag.
23- Ruttner, F., Pourasghar, D., and Kauhausen, D., 1985; Die Honigbienen Des Iran. Apis mellifera meda skorikow, Die Persische Biene. Apidologie,16: 241-264.
24- Saitou, N., and Nei, M., 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology Evolution,4: 406-425.
25- SAS/ STAT, CATMOD procedure (SAS version 9.1). SAS Institute  Inc., Cary.
26- Segura, J. A. L., 2000; Highly polymorphic DNA markers in an Africanized honey bee population in Costarica. Genetics and Molecular Biology,23: 317-322.
27- SplitsTree ver 4.10, 2011; Available at:  http://www.splitstree.org
28-  SPSS (IBM SPSS Statistics) ver 20.0, 2008; SPSS Inc., Chicago, Ill.
29- Ting, J., Ling, Y., Min, L., Wen-bin, B. and Guo- hong, C., 2008; Study on genetic diversity of Apis cerana and Apis mellifera ligustica in China with microsatellite markers. Research Journal of Animal Sciences,2: 178-182.
30- Wei, S., 2001; Genetic variation and colony development of honey bee (Apis mellifera) in            Kenya. Journal of Apicultural Research,29: 131-142.