Document Type : Research Paper
Authors
Abstract
An experiment was conducted with two hundred and twenty day-old male broiler chicks (Ross 308) in five groups to investigate the effects of different levels of 0, 0.2, 0.4 and 0.6% thyme extract in drinking water (including 27.4 mg sodium nitrate per liter) on broiler performance and antioxidantstatus and blood gas indices. No significant differences were observed between the treatments for body weight gain and feed consumption during the starter, grower, finisher and whole the experimental periods (P˃0.05). Nitrate consumption had no effect on feed conversion ratio during the starter period, but consumption of highest thyme level increased it (P˃0.05). There was no significant difference between the treatments for feed conversion ratio during the grower, finisher and whole the experimental periods (P˃0.05). None of the experimental treatments affected the blood uric acid, creatinine, total protein, malondialdehyde, total antioxidant capacity and blood gas indices at day 42 of age (P˂0.05). Nitrate consumption increased the blood urea whereas thyme extract supplementation decreased it (P˂0.05). It was concluded that consumption of 27.4 mg nitrate per liter drinking water causes the kidney injuries and higher blood urea in the end of the experimental period (day 42 of age) as a consequence but does not changes the performance, antioxidant indices and blood gas indicates, that shows the lack of induced methemoglobinemia and peroxidation. The thyme extract consumption in drinking water of broiler chicken decreases the blood urea through the diminished kidney injuries.
Alçiçek, A., Bozkurt, M. and Çabuk, M. )2004(. The effect of a mixture of herbal essential oils, an organic acid or a probiotics on broiler performance. South African Journal of Animal Science. 34: 217-222.
2- Atef, M., Abo-Norage, M.A., Hanafy, M.S. and Agag, A.E. (1991). Pharmacotoxicologicalaspects of nitrate and nitrite in domestic fowls. British Poultry Science. 32: 399-404.
3- Ayo, J.O., Minka, N.S. and Mamman, M. (2006). Excitability scores of goats administered ascorbic acid and transported during hot-dry conditions. Journal of Veterinary Science. 7: 127-131.
4- Barton, T.L., Hileman, L.H. and Nelson, T.S. (1986). A Survey of water quality on Arkansas broiler farms and meeting poultry health and Condemnation. University of Arkansas Fayetteville.
5- Baydar, H., Sagdic, O., Ozkan, G. and Karadogan, T. (2004). Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. Food Control. 15: 169-172.
6- Bölükbaşi, Ş.C., Erhan, M.K. and Özkan, A. (2006). Effect of dietary thyme oil and vitamin E on growth, lipid oxidation, meat fatty acid composition and serum lipoproteins of broilers. South African Journal of Animal Science. 36: 189-196.
7- Carter, TA. and Sneed, RE. (1987). Poultry Science and Technology Guide. North Carolina State University, Raleigh.
8- Choi, S.Y., Chung, M.J. and Sung, N.J. (2002). Volatile N nitrosamine inhibition after intake of Korean green tea and Maesil extracts with an amine-rich diet in subjects ingesting nitrate. Food and Chemical Toxicology. 40: 949-957.
9- Chow, C.K. and Hong, C.B. (2002). Dietary vitamin E and selenium and toxicity of nitrite and nitrate. Toxicology. 180: 195-207.
10- Chow, C.K. and Hong, Reese, M. and Gairola, C. (1984). Effect of dietary vitamin E on nitrite-treated rats. Toxicological Letters, 23: 109-117.
11- Cross, D.E., Acamovic, T., Deans, S.G. and McDevitt, R.M. (2002). The effect of dietary inclusions of herbs and their volatile oils on the performance of growing chickens. British Poultry Science. 43: 33-35.
12- Cross, D.E., McDevitt, R.M., Hillman, K. and Acamovic, T. (2007). The effects of herbs and their associated essential oils on performance, dietary digestibility and gut microflora in chickens from 7 to 28 days of age. British Poultry Science. 48: 496-506.
13- Cross, D.E., Svoboda, K., McDevitt, R.M. and Acamovic, T. (2003). The performance of chickens fed diets with or without thyme oil and enzymes. British Poultry Science. 44: 18-19.
14- Farag, R.S., Badei, A.Z.M.A., Hewedi, F.M. and El-Baroty, G.S.A. (1989). Antioxidant activity of some spice essential oils on linoleic acid oxidation in aqueous media. Journal of American Oil Chemists Society. 66: 792-799.
15- Jamroz, D., and Kamel, C. (2002). Plant extracts enhance broiler performance. Journal of Animal Science. 80: 41-41.
16- Kaneko, J.J., Harvey, J.W. and Michael, LB. (1997). Clinical Biochemistry of Domestic Animals. 5th Ed. New York, Academic Press. pp: 857-879.
17- Kasyanenko, I.V., Pinchuk, V.G., Mysasoyedov, D.V., Pivnyuk, V.M. and Osipova, L.A. (1992). Oncology. Naukova Dumka, Kiev. 264 pp.
18- Kolahi, S., Hejazi, J., Mohtadinia, J., Jalili, M., Farzin, H., (2011). The evaluation of concurrent supplementation with vitamin E and omega-3 fatty acids on plasma lipid per oxidation and antioxidant levels in patients with rheumatoid arthritis. Internet Journal of Rheumatology. 7:1cf7.
19- Lee, K.W., Everts, H., Kappert, H.J., Frehner, M., Losa, R. and Beynen, AC. (2003a). Effects of dietary essential oil components on growth performance, digestive enzymes and lipid metabolism in female broiler chickens. British Poultry Science. 44: 450-457.
20- Lee, K.W., Everts, H., Kappert, H.J., Frehner, M., Losa, R. and Beynen, A.C. (2003b). Dietary Carvacrol lowers body weight gain but improves feed conversion in female broiler chickens. The Journal of Applied Poultry Research. 12: 394-399.
21- Lee, K.G. and Shibamoto, T. (2001). Inhibiton malonaldehyde formation from blood plasma oxidation by aroma extracts and aroma components isolated from clove and eucalyptus. Food and Chemical Toxicology. 39: 1199-1204.
22- Lee, K.W., Everts, H., Kappert, H.J., van der Kuilen, J., Lemmers, A.G., Frehner, M., Beynen, A.C. (2004a). Growth performance, intestinal viscosity, fat digestibility and plasma cholesterol in broiler chickens fed a rye-containing diet without or with essential oil components. International Journal of Poultry Science. 3: 613-618.
23- Manassaram, D.M., Backer, L.C. and Moll, D.M. (2006). A review of nitrates in drinking water: Maternal exposure and adverse reproductive and developmental outcomes. Environmental Health Perspective. 114: 320-327.
24- Merino, Leonardo. (2008). Development and validation of a method for determination of residual nitrite/nitrate in foodstuffs and water after Zinc reduction. Food Anal Methods. 2: 212–220.
25- Marrett, L. E. and Sunde, M. L. (1968). The use of turkey poults and chickens as test animals for nitrate and nitrite toxicity. Poultry Science. 68:511-519.
26- Phillips, W.E. (1971). Naturally occurring nitrate and nitrite in foods in relation to infant methaemoglobinaemia. Food and Cosmetics Toxicology. 9: 219-228.
27- Rocha, BS., Gago, B., Barbosa, RM., Lundberg, J.O., Radi, R. and Laranjinha, J. (2012). Intra gastric nitration by dietary nitrite: implications for modulation of protein and lipid signaling. Free Radical Biology Medicine. 52: 693-8.
28- Salvemini, D. and Cuzzocrea. (2002). Superoxide, superoxide dismutase and ishemic injury. Current Opinion Investigational Drugs. 3:886-895.
29- Santamaria, P. (2006). Nitrate in vegetables: toxicity, content, intake and EC regulation (review). Journal of the Science of Food and Agriculture. 86: 10-17.
30- Sharafzadeh, Sh., Alizadeh, O. and Vakili, M. (2011). Effect of Nitrogen Sources and Levels on essential oil components of thymus vulgaris L. Australian Journal of Basic and Applied Sciences. 5: 885-889.
31- Singhal, S., Gupta, R. and Gogle, A. (2001). Comparison of antioxidant efficacy of vitamin E, vitamin C, vitamin A and fruits in coronary heart diseases. A controlled trial. Journal of the Association of Physicians, India. 49: 327-331.
32- Slepchenko, V.N. and Rhnelnitskh, G.A. (1988) Toxic effects of dietary nitrate on semen production in bulls. Veterinariya, Kiev USSR. 63:63-67.
33- Suteu, R., Altuntas, I., Buyukvanli, B., Akturk, O., Koylu, H. and Delibas, N. (2007). The effects of diazozin on lipid peroxidation and antioxidant enzymes in rats erythrocytes: role of vitamins E and C. Toxicology and Industrial Health. 23: 13-17.
34- Toghyani, M., Tohidi, M. (2010). Performance, immunity, serum biochemical and hematological parameters in broiler chicks fed dietary thyme as alternative for an antibiotic growth promoter. African Journal of Biotechnology. 9: 6819-6825.
35- Ulfah, M. (2006). Essential oils as multi-function feed additive (MFA) to improve broilers performance, metabolism, dung consistency and efficiency of production. Journal of Agriculture and Rural Development in the Tropics and Subtropics. 88: 50-55
36- Youdim, K.A. and Deans, S.G. (2000). Effect of thyme oil and thymol dietary supplementation on the antioxidant status and fatty acid composition of the aging rat brain. British Journal of Nutrition. 83: 87-93.
37- Youdim, K.A. and Deans, S.G. (1999). Dietary supplementation of thyme (Thymus Vulgaris L.) essential oil during the lifetime of the rat: Its effects on the antioxidant status in liver, kidney and heart tissues. Mechanisms of Ageing and Development. 109: 163-75.
38- Youdim, K.A., and Deans, S.G. (1999). Beneficial effects of thyme oil on age-related changes in the phospholipid C20 and C22 polyunsaturated fatty acid composition of various rat tissues. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 1438: 140-6.
39- Zhang, KY., Yan, F., Keen, CA. and Waldroup, PW. (2005). Evaluation of microencapsulated essential oils and organic acids in diets for broiler chickens. International Journal of Poultry Science. 4: 612-619.
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