Effects of extender and sperm chamber on computer-assisted analysis of sperm motility (CASA) in goats

Document Type : Research Paper

Authors

Abstract

Computer-assisted semen analysis (CASA) is used as an accurate and objective tool for kinetic sperm measurements. The aims of this study were evaluate the effects of extender and sperm chamber on motility parameters of goat sperm using CASA. Semen collected from five Mahabadi goats via artificial vagina. The collected semen divided in two fractions and diluted in two commercially soyabean based extender, Bioxcell and Andromed to finally concentration of 6 × 106 spermatozoa per ml. The diluted semen evaluated via CASA and using two sperm chambers including Makler chamber with 10µm deep and Leja chamber with 20µm deep, and microscopic slide with 18*18mm cover. The motility parameters considered in this study were average path velocity (VAP, µm/s), straight line velocity (VSL, µm/s), curvilinear velocity (VCL, µm/s), amplitude of lateral head displacement (ALH, µm), beat cross frequency (BCF, Hz), straightness (STR, as VSL/VAP, %), and linearity (LIN, as VSL/VCL, %). The medium used for the dilution of the sample significantly affects all sperm motility parameters except VCL. Only ALH was significantly lower (P < 0.05), whereas VSL, VAP, BCF LIN and STR were significantly higher, in the semen diluted in Bioxcell than Andromed. The effects of sperm chamber were significant only in LIN and BCF. In conclusion the extender had significant effect on the CASA results whereas sperm chamber didn't  has so effects.

Keywords

References

مصطفی پور، س (1388) مطالعه تأثیر نوع رقیق کننده، میزان رقیق سازی و مدت نگهداری منی در آنالیز کامپیوتری تحرک اسپرم قوچ، پایان نامه کارشناسی ارشد، دانشگاه ارومیه. شماره پایان نامه: 119-2ک.
Amann, R. ( 1989 ). Can the fertility potential of a seminal sample be predicted accurately. Journal Androl, Vol, 10, No, 2. pp: 89-98.
Budworth, P. Amann, R. and Chapman, P. ( 1988). Relationships between computerized measurements of motion of frozen thawed bull spermatozoa and fertility. Journal Androl, Vol, 9, No, 1. pp:41-54.
Contri, A. Valoizb, C. Faustinic, M. Wegherb, L. and Carluccioa A. (2010). Effect of semen preparation on casa motility results in cryopreserved bull spermatozoa. Theriogenology, Vol, 74, No, 3. pp: 424–435.
Cox, J. Alfaro, V. Montenegro, H. and Rodriguez- Martinez, H. (2006). Computer- assisted analysis of sperm motion in goats and its relationship with sperm migration in cervical mucus. Theriogenology, Vol, 66, No. pp: 860- 867.
Dorado, J. Rodriguez, I. and Hidalgo, M. (2007). Cryopreservation of goat spermatozoa: Comparison of two freezing extenders based on post-thaw sperm quality and fertility rates after artificial insemination. Theriogenology, Vol, 68, No, 2. pp: 168–177.
Farrell, P.Foote, R. Mc Ardle, M. Trouem-Trend, V. and Tardif, A. (1996). Media and dilution procedures tested to minimize handling effects on human, rabbit, and bull sperm for computer-assisted sperm analysis (CASA). Androl Journal, Vol, 17, No, 3. pp: 293-300.
Iguer-Ouada, M. and Verstegan, J. (2001). Evaluation of the Hamilthon- Thorn computer based automated system for dog semen analysis. Theriogenology, Vol, 55, No, 3. pp: 733-749.
Quintero-Moreno, A., Rigau, T., Rodríguez-Gil, J., (2004). Regression analyses and motile sperm subpopulation structure study as improving tools in boar semen quality analysis. Theriogenology, Vol, 61,No, 4. pp: 673–690.
Robayo, I. Montenegro, V. Valde’s, C. and Cox, J. (2008). CASA assessment of kinematic parameters of ram spermatozoa and their relationship to migration efficiency in ruminant cervical mucus. Reproduction Domestic Animal Journal, Vol, 43, No, 4. pp:393- 399.
Schafer- Somi S, Aurich CH. ) 2007 .(Use of new computer- assisted sperm analyzer for the assessment of motility and viability of dog spermatozoa and evaluation of four different semen extenders for predilution. Animal Reproduction Science, Vol, 102, No, 1-2. pp: 1- 13.
Tardif A.L, Farrel P.B, Trouern- Trend, Footel R.H.) 1997 .(Computer- assisted sperm analysis for assessing initial semen quality and changes during storage at 5˚C. J Dairy Sci, Vol, 80, No, 8. pp: 1606- 1612
Varner D.D., Vaughan S.D., Johnston L. (1991). Use of a computerized system for evaluation of equine spermatozoa1 motility. Am J Vet Res, Vol, 52, No, 2. pp: 224-228.
Verstegen, J. (2002). Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology, Vol, 57, No, 1. pp: 149-179.

Files

Share

How to cite

Statistics