Association between Polymorphism IGF-I gene exon 1 and growth traits in Moghani Sheep

Document Type : Research Paper

Authors

Abstract

In this study, 100 Moghani sheep from breeding station located in Jaafarabad, Moghan were genotyped for exon 1 of IGF-I gene, using PCR-SSCP. Different band patterns were obtained in this site gene that was studied. Based on SSCP patterns, three genotypes BC, BD and BB obtained with frequencies of 65%, 26% and 9%, respectively. The genotype frequencies had a significant deviation from HWE. In addition, significant associations were observed between BB, BD and BC genotypes and weaning weight, weight in six months, weight in nine months, Average daily gain and Kleiber ratio. No association of the genotypes with the Birth weight and Yearling weight traits were found. These association polymorphisms and growth traitsmay be applied in sheep breeding schemes as marker assisted selection (MAS).

Keywords

References

اسدی خشویی، ا.، میزائی آشتیانی،  س. ر.، ترکمن زهی،آ.(1378). ارزیابی نسبت کلیبر (Kleiber-Ratio) به عنوان یکی از معیارهای انتخاب قوچ در گوسفند نژاد لری بختیاری. مجله علوم کشاورزی ایران. جلد 30، شماره 4، ص 655-649.
یزدان پناه، ا.، خدرزاده، ع.، محمدی کفتر کاری، م. (1389). بررسی چند شکلی ژن IGF-I در جمعیت گاومیش استان خوزستان با استفاده از تکنیک PBR. چهارمین کنگره علوم دامی کشور.
کارگر، ن.، مرادی شهر بابک، م.، مروج، ح.، رکوعی، م. (1385)، تخمین پارامترهای ژنتیکی صفات رشد و پشم در گوسفند کرمانی. مجله پژوهش و سازندگی، شماره 73، ص 95-88.
رشیدی، ا.، افتخار شاهرودی، ف.، نیکخواه، ع.، اصغری، ی. (1377). تخمین پارامتر­های ژنتیکی و فنوتیپی صفات رشد در گوسفند مغانی. مجله علوم کشاورزی ایران. جلد 29، شماره 2، ص 235-227.
Bale, L.K. and Conover, C.A. (1992). Regulation of insulin Like growth factor binding protein-3 messenger ribonucleic acid expression by insulin-like growth factor I. Endocrinology, Vol, 131, No, 2. pp: 608-614.
Benbouza, H. Jacquemin, M. Baudoin, J. and Mergeai, G. (2006). Optimization of a reliable, fast, cheap and sensitive silver staining method to detect SSR markers in polyacrylamide gel. Biotechnology, Agronomy, Society and Environment, Vol, 10, No, 2. pp: 77-81.
Beuzen, N., D. STEAR M. J. and Chang, K. C.( 2000). Molecular markers and their use in animal breeding. The Veterinary Journal. Vol,160, No, 1: pp. 42–52.
Byrne, P.F. and McMullen, M. D. (1996). Defining genes for agricultural traits: QTL and the candidate gene approach. Probe, Vol, 7, No, 3. pp:24–27.
Dekkers, J. C. M. and Hospital, F. (2002). The use of molecular genetics in improvement of agricultural populations. Nature Reviews Genetics. Vol, 3, No, 1. pp: 22-32.
Ge, W. Davis, M. E. and Hines, H. C. (1997). Two SSCP alleles detected in the 5'-flanking region of bovine IGF1 gene. Animal Genetics. Vol, 28, No, 2. pp: 155-156.
Ge, W. Davis, M. E. Hinec, H. C. Irvin, K. M. and Simmen, R. C. M.(2001). Associations of a genetic marker with blood serum insulin-like growth factor-1 concentration and growth traits in angus cattle. Journal of Animal Science, Vol, 79, No, 7. pp: 1757-1762.
Honarvar M., M. Sadeghi., H.  Moradi-Shahre babak, S.H. Behzadi., H. Mohammadi and  A. Lavaf. 2012.Study of polymorphisms in the 5" flanking region of the ovine IGF-I gene in Zel sheep. World Applied Sciences Journal. Vol, 16, No, 5. pp: 726-728.
Imam-Ghali, M.I. Saidi-Mehtar, N. and Guerin, G. (1991). Sheep gene mapping: additional DNA markers included (CASB, CASK, LALBA, IGF-I and AMH). Animal Genetics. Vol, 22, No, 2. pp:165-172.
Javanrouh, A. Banabazi, M.H. Esmaeilkhanian, S. Amirinia, C. Seyedabadi, H. R. and Emrani, H. (2006) Optimization on salting out method for DNA extraction from animal and poultry blood cells. The 57th Annual Meeting of the European Association for Animal Production. Antalya, Turkey 21-25 August.
Kazemi, S. M. Amirinia, C. Emrani, H. and Gharahveysi, SH. (2011). Study and    Identification of Insulin-Like Growth Factor-I Gene Polymorphisms in Zel Sheep Population. American Journal of Animal and Veterinary Sciences .Vol, 6, No, 4. pp: 176-179.
Pipalia, D.l. Joshi, C.G. Brahmkshtri, B.P. and Sonlanki, J.V. (2004). PCR-SSCP typing of MHC in cattle and buffaloes. Indian Journal of Animal Sciences,Vol, 74, No, 6. pp: 637-639.
Qiong, W. Chao, F. Wu-Jun, L. Yi, F. and Shi-Gang, Y. (2011). A Novel Mutation at Exon 4 of GENE IN Three Indigenous Goat Breeds in China.Asian Journal of Animal and Veterinary Advances. Vol, 6, No, 6. pp: 627-635.
Roite, D. Bondy, C. Yakar, S. Liu, J.L. and Butler, A. (2001). The Somatomedin Hypothesis. Endocrine Reviews. Vol, 22, No, 1. pp: 53-74.
Reyna, X.F. Montoya, H.M. Castrellon, V. V. Rincon, A.M. Bracamonte, M.P. and Vera, W. A. (2010). Polymorphisms in the IGF1 gene and their effect on growth traits in Mexican beef cattle. Genetics and Molecular Research. Vol, 9, No, 2. pp:875-883.
SAS. (2008). User's Guide: Statistics. Version 9.1, Cary, NC, USA.
Shimatsu, A. and Rotwein, P. (1987). Mosaic evolution of the insulin-like growth factors. Organization, sequence, and expression of the rat insulin-like growth factor I gene. Journal of Biological Chemistry. Vol, 262, No, 16. pp: 7894-7900.
Tahmoorespur, M. Valeh, M.V. Nassiry, M.R. Heravi Moussavi, A. and Ansary, M. (2009). Association of the polymorphism in the 5’ flanking  region of the ovine IGF-I gene with growth traits in the Baluchi sheep. S Afr. Journal of Animal Science. Vol, 39, No, 5. pp: 97-101.
Tosh, J.J. and Kemp, R.A. ( 1994). Estimation of variance components for lamb weights in three sheep populations. Journal of Animal Science. Vol, 72, No, 5. pp: 1184-1190.
Yazdi, M.H. Engstrom, G. Nasholm, A. Johansson, K. Jorjani, H. and Liljedahl, L.E. (1997). Genetic parameters for lamb weight at different ages and wool production in Baluchi sheep. Journal of Animal Science. Vol, 65, No, 2. pp: 247-255.
 Yeh, F. C. Yang, R. and Boyle, T. (1999). POPGENE (V. 1.31). University of Alberta and Centre for International Forestry Research. USA
Yilmaz, A. E. Michael, C. H.  Harold, Hines and Hoyoung. Chung. (2005). Detection of two nucleotide substitutions and putative promoters in the 5’ flanking region of the ovine IGF-I gene. Journal of Applied Genetics. Vol, 46, No, 3. pp: 307-309.
Zhang, Ch. Zhang, W. Luo, H. Yue1, W. Gao, M and Jia, Z. (2008). A new single nucleotide polymorphism in the IGF-I gene and its association with growth traits in the Nanjiang Huang goat.Asian-Australasian Journal of Animal Sciences. Vol, 21, No, 8. pp: 1073-1079.

Files

Share

How to cite

Statistics