The effect of egg yolk from different birds in Tris diluent on the quality of Arabic ram semen in liquid condition

Document Type : Research Paper

Authors

1 , Ramin Agriculture and Natural Resources University of Khuzestan.

2 Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khuzestan, Iran.

3 , Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resources University of Khuzestan, Iran.

Abstract

This experiment were conducted in order to compare the protective effect of egg yolk from 6 species of birds [industry chicken (Gallus Gallus), local chicken (Gallus Gallus domesticus), duck (Anatidae anas platyrhynchos), goose (Anatidae anser), quail (Coturmix japonica) and pheasant (Phasianus Colchicus)] to protect Arabian ram semen in four times (1, 6, 12 and 24 hours) of semen storage under liquid condition (5°C), was done in a completely randomized design with six treatments (Egg yolks of 6 birds) and eight replications. Semen samples from 10 Arabic rams with 2-3 years were collected weekly in breeding season for eight weeks with using electric shock and semen samples were mixed. Semen samples were mixed with Tris extender containing eggs yolks of different treatments (14%) and then the percentage of motility, viability and morphological abnormalities of spermatozoa in four times after dilution were evaluated. The result of this experiment showed that, use duck egg yolk, goose and local chicken, positive effect on the reduction in percentage of spermatozoa motility and viability during storage at quarter time (24 hours) of (P<0.05). The passage of time (six hours of storage) duck egg yolk and quail have the maximum effects on the survival (P<0.05). The percentage of morphological abnormalities increased with the passage of time and the use of different sources egg yolks not to make a significant difference. In general, the results showed that the duck egg yolk, goose eggs and chicken local, have better protective effect for Arabian ram semen stored in liquid condition.

Keywords


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