Effect of Enterococcus facium isolates from intestine of Coracias garrulus anf lactofeed probiotic on performance, blood biochemical, intestine morphology and microflora of Japanese Quail

Document Type : Research Paper

Authors

Abstract

The purpose of this study was to investigate the effect of acidophilus bacterial isolates from intestine of Coracias Garrulus on performance and carcass characteristics parameters of Japanese quail. A total of 385 chicks were arranged into 35 experimental units with 7 treatments in a completely randomized design. The treatments were included control, spraying, drinking and sprying+ drinking of the acidophilus bacterial isolates, and spraying, drinking and sprying+ drinking of commercial probiotic. Two birds from each replicate were sacrificed and bleeding, then blood was gathered and plasma was extracted.The results were revealed that addition of acidophilus bacterial isolates and commercial probiotic did not affect feed intake at 47 d. The body weight was increased when birds received acidophilus bacterial isolates and commercial probiotic. The FCR were lower in birds received drinking probiotic and in spraying+ drinking acidophilus bacterial isolates treatments as compared to control. The carcass parameters did not affected by treatments. The results were revealed that addition of acidophilus bacterial isolates and commercial probiotic were decreased the serum cholesterol and LDL concentration of quail. The HDL and triglyceride were not affected by treatments. The protein concentration were increased in chicks received the acidophilus bacterial isolates and commercial probiotic. The antibody response against SRBC and IgM were increased in birds received acidophilus bacterial isolates or commercial probiotic. The jejuna histomorphmetry were showed that height of villi, crypt depth, and the ratios of height of villi to crypt depth were significantly changed.

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بذرافشان ،خ. کریمی ترشیزی ،م.ا. رحیمی،ش. (1391). تاثیر برخی باکتری های جدا شده از پروبیوتیک های تجاری بر رشد، ترکیب لاشه و سیستم ایمنی بلدرچین ژاپنی. مجله پژوهش و سازندگی (نشریه علوم دامی). 96: 15-24.
تشفام، م.، رحیمی، ش.، و کریمی، ک. (1384). تأثیر سطوح مختلف پروبیوتیک بر مورفولوژی مخاط روده جوجه­های گوشتی. مجله دانشکده دامپزشکی، دوره 60 :211- 205.
سلطانی، م.، مظهری، م.،‌ و اسماعیلی پور، ا. ع. (1394) اثر سطوح گوناگون پروبیوتیک کلوستات بر عملکرد و ایمنی جوجه های گوشتی تحت تنش گرمایی در دورۀ پایانی. تولیدات دامی. 17(2):‌ 291-300.
شمس شرق، م.، و خسروی، ع.، (1390). افزوودنی­های خوراکی در تغذیه طیور. چاپ اول. دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ص ص: 15-130.
کریمی ترشیزی، م. ا.، سیفی، ک.، رحیمی، ش. و یوسفیان، س. (1392). بررسی کارآیی پروبیوتیک بر عملکرد رشد بلدرچین ژاپنی. همایش ملی دام و طیور، 12 اردیبهشت 1392، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ص. 769-772.
لطفی، ح.، حجازی، م. ا.، ملکی زنجانی، ب.، و برزگری، ا.‌ (1389) جداسازی، شناسایی بیوشیمیایی و مولکولی باکتریهای با پتانسیل پروبیوتیکی از محصولات لبنی سنتی مناطق هریس و سراب. مجله پژوهش‌های صنایع غذایی. 3: 20.
عبدی نژاد، م. (1395). روش‌های مقابله با پرنده زنبورخوار (سبزقبا). انتشارات موسسه محیط زیستی سرزمین ایده آل ما.
مظفری قله جوق، م.، گلیان، ا.، و کرمانشاهی، ح. (1392) اثر پروبیوتیک پدیوکوکوس‌اسیدی‌لاکتیسی بر عملکرد، قابلیت هضم مواد مغذی، بافت‌شناسی و فلور میکروبی روده در جوجه‌های گوشتی. پایان نامه دانشگاه فردوسی مشهد.
 Ahmad, I. (2006). Effect of probiotics on broilers performance. International Journal of Poultry Science. 5: 593-597.
Aviagen. (2009). Ross 308 broiler management guide.Aavailable at www.aviagen.com
Axelsson, L. (1998). Lactic acid bacteria: classification and physiology. In: Salminen, S. and VonWright, A., eds. Lactic acid bacteria: microbiology and functional aspects. 2nd edition. New York. Marcel Dekker Inc. pp 1-72.
Ayasan, T., and Okan, F. (2001). The effect of a diet with different probiotic (Protexin) levels on the fattening performance and carcass characteristics of Japanese quails. Proceedings of XVth European Symposium on the Quality of Poultry Meat. pp: 169-174. Kuþadasi, Turkey.
Chichlowski, M., Croom, W.J., Edens, F.W., MacBride, B.W., Qiu, R., Chiang, C.C., Daniel, L.R., Havenstein, G.B. and Koci M.D. (2007) Microarchitecture and spatial relationship between bacteria and ileal, cecal and colonic epithelium in chicks fed a direct-fed microbial, PrimaLac, and salinomycin. Poultry Science. 86: 1121-1132.
Dhama, K., Tiwari, R., Khan, R.U., Chakraborty, S., Gopi, M., Karthik, K., Saminathan, M., Desingu, P.A., and Sunkara, L.T. (2014). Growth promoters and novel feed additives improving poultry production and health, bioactive principles and beneficial applications: The Trends and Advances-a Review. International Journal of Pharmacology. 10: 129-159.
Ferket, P.R. (2002). Use of oliosacccharidies and gut modifiers as replacements for dielary antibiotics. Proceeding 63rd  Minnesota nutrition Conference, Septembr 17-18, Eagan, MN, pp 169-182.
Fuller, R. (1977). The importance of Lactobacilli in maintaining normal microbial balance in the crop. British Poultry Science. 1: 85-94.
Gill, H., Rutherfurd, G. and Cross, M. (2001). Dietary probiotic supplementation enhances natural killer cell activity in the elderly. Journal of Clinical Immunology. 21(4):264- 271.
Gunal, M., Yayli, G., Kaya O., Karahan, N., and Sulak, O. (2006). The Effects of antibiotic Growth Promoter, Probiotic or Organic Acid Supplementation on Performance, Intestinal Microflora and Tissue of Broilers. International Journal of Poultry Science. 5:62.
Haghighi, H.R., Gong, J., Gyles, C.L., Hayes, M.A., Sanei, B., Parvizi, P., Gisavi, H., Chambers, J.R. and Sharif. S. (2005). Modulation of antibody-mediated immune response by probiotics in chickens. Clinical Diagnostic Laboratory Immunology. 12: 1387–1392.
Jin, L. Z., Abdullah, N. and Jalaleldin, S. (1998). Growth performance, intestinal microbial population and serum cholesterol of broilers diets containing Lactobacillus cultures. Poultry Science, 77: 1259-1265.
Macfaddin, (1985). Media for isolation- cutivaton- indentification- maintenance bacteria, vol.1. willwams and wilkins, Baltiore, MD.
Mountzouris KC, Tsitrsikos P, Palamidi I, Arvaniti A, Mohnl M, Schatzmayr G and Fegeros K (2010). Effects of probiotic inclusion levels in broiler nutrition on growth performance, nutrient digestibility, plasma immunoglobulins, and cecal microflora composition. Poultry Science. 89: 58-67.
Nelson, N.A., Lakshmanan, N., Lamoni, S.J. (1995). Sheep red blood cell and Brucella abortus antibody responses in chickens selected for multitrait immunocompetence. Poultry Science. 74: 1603-1609
Panda, A. K. Reddy, M.R., RamaRao, S.V., Raju. M.V.L.N., and Paraharaj, N.K. (2000). Growth, carcass characteristics, immunocompetence and  response to Escherchia coli on broiler fed diets with various level of probiotic .Archive fur. Geflugelqunde. 64: 152-156.
SAS institute, (2003), SAS/STAT®, user’s guide, release 9.1 edition, SAS institute Inc, cary, NC.
Sharifi, S.D., Dibamehr, A., Lotfollahian, H. and Baurhoo, B. (2012). Effects of flavomycin and probiotic supplementation to diets containing different sources of fat on growth performance, intestinal morphology, apparent metabolizable energy, and fat digestibility in broiler chickens. Poultry Science. 91: 918-927.
Saeed, A.K., Mohamad, S.N., and Ashraf, A.K. (2005). Selective isolation of multi drug resistant Enterococcus spp. From poultry and dairy farms: Detection of virulence and vancomycin resistance gene markers by PCR. Molecular Cellular Probes. 19: 27-34.
Strompfova, V., Marcinakova, M., Gancarcikova, S., Jonecova, Z., Scirankova, L., Guba, P., Koscova, J., Boldizarova, K., and Laukova, A. (2005). New probiotic strain Lactobacillus fermentum AD1 and its effect in Japanese quail. Veterinary Medicina,(Czech). 50(9): 415–420.
Vahdatpour, T., Nikpiran, H., Babazadeh, D., Vahdatpour, S. and Jafargholipour, M.A. (2011).  Effects of Protexin, Fermacto and combination of them on blood enzymes and performance of Japanese quails (Coturnix Japonica). Annals of Biological Research. 2(3): 283-291.