Sequencing and bioinformatics analysis of the exon 9 of Prolactin receptor gene in Khalkhali goat

Document Type : Research Paper

Authors

Abstract

Due to a crucial role in transferring of Prolactin hormone signals to milk protein gene promoter, Prolactin receptor (PRLR) gene has been detected as the most important candidate gene. The present study investigated the genetic variation of exon 9 of PRLR gene and bioinformatics analysis of this gene in farm animals. For bioinformatics analysis, 37 samples of different farm animal species genomic sequences were obtained. A mutation was detected in exon 9 of Khalkhali goat PRLR gene that causes to the substitution of A to G nucleotide and, led to change ATG codon to GTG codon with the substitution of Metioning amino acid with Valin. The results of analyzing of Exon 9 led to identify 28 polymorphic sites in the PRLR gene that causes 10 haplotypes with 0.8408 haplotype diversity in studied species. Nucleotide diversity and average nucleotide differences among species were 0.04439 and 10.655 respectively. Analysis of genetic diversity showed that ovine species have the more diversity compared with other species. Also, analysis of species distance and network analyses showed that ovine is most closed species to Capra than other species, and Khalkhali goat belongs to the same phylum as Capra Hircus.

Keywords

References

خالداری، م.(1382). اصول پرورش گوسفند و بز.چاپ دوم. انتشارات دانشگاه تهران.
علیجانی، ص. (1388). ردیابی ژنهای عمده در حیوانات مزرعه­ای با استفاده از آمار بیزی و نشانگرهای مولکولی. پایان نامه دکتری. دانشکده کشاورزی کرج-دانشگاه تهران.
Abula, R., Zhang. H.L, Chen, Y. and Yao, X.K. )2013(. Novel polymorphism detected in the prolactin receptor gene of Yilli horse (Equus caballus) by PCR-SSCP. Journal of Animal and Feed Science. 22: 70-76.
An, X., Hou, J., Gao, T., Lei, Y., Li, G., Song, Y., Wang, J. and Cao, B. (2015). Single-nucleotide polymorphisms g. 151435C> T and g. 173057T> C in PRLR gene regulated by bta-miR-302a are associated with litter size in goats. Theriogenology. 83, 1477-1483. e1471.
Arden, K.C., Boutin, J.M., Djiane, J., Kelly, P.A. and Cevenee, W.K. )1990(. The receptor for Prolactin and Growth hormone are localize in the same region of the human chromosome 5. Cyto Genetic Cell Genetic. 53: 161-165.
Bole-Feysote, G., Goffin, V., Edery, M., Binart, N. and Kelly, P.A. )1998). Prolactin (PRL) and its receptor: actions, signal transduction pathways and phenotypes observed in PRL receptor knockout mice. Endocrine Reviews. 19(3), 225-268.
Bryme, P., Kaminski, S. and Wojcikk, E. )2005(. Nucleotide sequence polymorphism within exon 4 of bovin prolactin gene and its association with milk performance traits. Journal of Applied Genetics. 45: 179-185.
Chen, Y., Luo, Q.J., Yang, J.Q., Yang, F.Y., Zhang, Y. J., Li, D.Z. and Zhu, W.Y. (2010). Relationships between genetic polymorphisms of intron 9 and exon 10 of prolactin rceptor gene and litter size of sheep. China Animal Husbandry and Veterinary Medicine. 37, 100-106.
Di, R., Yin, J., Chu, M., Cao, C.L., Feng, T., Fang, L., and Zhou Z.X. )2011(. DNA polymorphism of introns 1 and 2 of Prolactin Receptor Gene and its association with litter size in goats. Animal Science Papers and Reports. 4:343-350
Hayes, H., Le Chalony, C., Goubin, G., Mercier, D., Payen, E., Bignon, C., & Kohno, K. (1996). Localization of ZNF164, ZNF146, GGTA1, SOX2, PRLR and EEF2 on homoeologous cattle, sheep and goat chromosomes by fluorescent in situ hybridization and comparison with the human gene map. Cytogenetic and Genome Research. 72(4), 342-346.
Hou, J.X., Fang, F., An, X.P., Yan, Y., Ma, T., Han, P., Meng, F., Song, Y.X., Wang, J.G. and Cao, B.Y. )2014(.  Polymorphisms of PRLR and FOLR1 genes and association with milk production traits in goats. Genetics and Molecular Research. 13 (2): 2555-2562.
 Hou, J., An, X., Han, P., Peng, J., and Cao, B. (2015). Two missense mutations in exon 9 of caprine PRLR gene were associated with litter size. Animal Genetics. 46(1), 87-90.
Hou, J., An, X., Song, Y., Wang, J., Ma, T., Han, P. and Cao, B. (2013). Combined effects of four SNPs within goat PRLR gene on milk production traits. Gene. 529(2), 276-281.
Khabiri, A.A., Tahmoorespur, M., Nassiri, M.R., and Hadi, M. (2014). Sequencing and bioinformatics analysis of the partial promoter region of κ-casein (CSN3) gene in Iranian Bacterianus and Dromedaries camels. International Journal of Advanced Biological and Biomedical Research. 2, 1719-1725
Li, Y., Zhang, L., Shang, L., Wang, H., Zou, H., Zhang, H., and Ji, D. (2010). Genetic polymorphisms at three loci of PRLR and FSHR gene correlate with litter size in Chinese Haimen goat. Journal of Animal and Veterinary Advances. 9(22), 2835-2838.
Song, X., Xu, C., Yue, Z., Wang, L., Wang, G., and Yang, F. (2015). Bioinformatic analysis based on the complete coding region of the MSTN gene within and among different species. Genetics and molecular research: GMR. 15(2).
Sun, R.P., Wang, L.X., Zhu, G.Q., Wang, J.G., Song, Y.X., Liang, Z.Y. and CAO, B.Y. (2008). Polymorphism of Exon 10 of Prolactin Receptor Gene and Its Relationship with Milk Performance of Xinong Saanen Dairy Goat. Chinese Journal of Animal and Veterinary Sciences. 12, 004.
Tajima, F. )1989(. Statistical method for testing the neutral mutation hypothesis by dna polymorphism. Genetics. 123: 585-595.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S. )2011(. MEGA5: molecular evolutionary genetics analysis using maximum likelihood evolutionary distance, maximum parsimony methods. Molecular Biology Evolution. 28(10):2731-2739..
Trott, J.F., Freking, B.A. and Hovey, R.C. (2014). Variation in the coding and 3′ untranslated regions of the porcine prolactin receptor short form modifies protein expression and function. Animal Genetics. 45, 74-86.
Viitala, S., Szyda, J., Blott, S., Schulman, N., Lidaure, M., Maki-Tanila, S., Georges, M. and Vilkki, J. )2006(. The Role of the Bovine Growth Hormone Receptor and Prolactin Receptor Genes in Milk, Fat and Protein Production in Finnish Ayrshire Dairy Cattle. Genetics. 173: 2151–2164.
Wu, X.H., Mu, X.K., Lu, J.Y., Wang, Y. and Zi, X.D. (2014). mRNA expression, polymorphism of prolactin receptor gene and their association with prolificacy in Lezhi black goats. Journal of Applied Animal Research 42, 414-419.
Zhang, J.L., Zan. L.S., Fang. F., Zhang. F., Shen. G. and Tian. W.Q. )2008(. Genetic variation of PRLR gene and association with milk performance traits in dairy cattle. Canadian Journal of Animal Science. 88: 33-39.
Animal Sciences Journal
Volume 30, Issue 115
September 2017
Pages 117-126

Files

Share

How to cite

Statistics