Document Type : Research Paper
Authors
Abstract
In this study to gain insights into transcriptional regulation Lactogenesis in mammary tissue, at the first high expression genes in lactogenesis in three different condition were identified by RNA-Seq data analysis. This three conditions including comparison of the dairy cow mammary tissue in 35 days before and 3 days after birth, dairy cow mammary tissue in 7 days before and 3 days after birth and dairy cow mammary tissue in 7 and 35 days before birth. To investigate the biological processes of significant high expression genes in three different condition mentioned above, the DAVID software was used. The only significant terms (P<0.05) related to biological processes were considered. Then promoter regions of these genes were extracted to identify candidate transcription factors involved in this mechanisms. Assuming that changes in the transcription level of co-expressed genes may result from the coordinated action of a limited number of transcription factors, we looked for over-represented putative transcription factor binding sites in the promoters of these genes. Promoter analysis by using Genomatix software lead to identify of 24 novel transcription factors candidates activating in lactogenesis. In this study in order to visualization of regulatory networks containing transcription factors and that regulate the genes, STRING software were used. According to the results, 24 new candidates transcription factor introduced in this study has good potential for more investigation in the laboratory.
Keywords
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