Effect of different levels of glutamine amino acid on semen quality of Arabi ram during different storage times under liquid condition in 5°C

Document Type : Research Paper

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Abstract

Glutamine is an amino acid which has antioxidant role. The aim of this study was to investigate the effect of different levels of glutamine into diluent on semen characteristics of Arabi ram in liquid condition. Semen samples were collected weekly from 8 Arabi rams for 8 weeks and pooled. After dilution, the pooled semen was divided into 5 parts and received the levels of glutamine (0 or control, 25, 50, 75 and 100 mM). In liquid storage condition under 5°C and different times (1, 24, 48, 72 and 96 hours), semen quality characteristics were evaluated. The glutamine levels have no effects on semen quality in first hour of maintenance (P>0.05). The sperm motility was not affected by treatments during 24 and 48 hours; But, sperm viability in control was lower than 25 mM glutamine (P<0.05). After 72 hours, the lowest sperm motility and viability (except 100 mM of glutamine) was observed in control. While, after 96 hours, only 25 mM and 50 mM dose of glutamine improved the spermatozoa motility and viability (P<0.05). After 24 hours, all glutamine levels were reduced the morphological defect rates of spermatozoa. But, over time, lower levels of glutamine (25 and 50 mM) reduced the morphological defect rates and improved the plasma membrane integrity of spermatozoa (P<0.05). In all times, pH of semen was not affected by treatments (P>0.05). Overall, the use of glutamine especially dose of 25 and 50 mM significantly increased the sperm quality parameters of Arabi ram without effect on semen pH.

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References

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Animal Sciences Journal
Volume 30, Issue 115
September 2017
Pages 233-242

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