Document Type : Research Paper
Authors
Abstract
This study was designed to investigate the effects of various concentrations of egg yolk (5, 10, 15 and 20%) and Lecithin (1, 1.5 and 2%) in the extenders based on tris (with 5% glycerol) on the Marghoz goat spermatozoa after freezing-thawing. Semen samples were collected by an artificial vagina, twice a week from four matured goat.The extender containing semen was frozen in liquid nitrogen and then was stored until using for assessment. Semen was thawed at 37◦C and then motility and progressive motility were assessed by CASA. Membrane integrity and viability,morphology were assessed as well and Malondialdehiyde (MDA) were were assessed as well. The results of this experiment showed that extender containing 15% egg yolk and 1.5% Lecithin significantly improved motility, progressivemotility and viability compare to other levels (P<0.05). Membrane integrity, morphology, malondialdehiyde and acrosome status were not affected significantly by the different level of egg yolk and Lecithin (P > 0.05). It can be concluded that use of 15% egg yolk in animal extender and 1.5% Lecithin in vegetable extender is suitable for cryopreservation of Marghoz goat semen.
Keywords
Aires, V.A., Hinsch, K.D., Mueller-Schloesser, F., Bogner, K., Mueller-Schloesser, S. and Hinsch, E. (2003). In vitro and in vivo comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of bovine semen. Theriogenology. 60: 269-279.
Amirat, L., Tainturier, D., Jeanneau, L., Thorin, C., Gérard, O., Courtens, J.L. and Anton, M. (2004). Bull semen in vitro fertility after cryopreservation using egg yolk LDL: a comparison with Optidy commercial egg yolk extender. Theriogenology. 61: 895-907.
Amoah, E.A. and Gelayes, S. (1997). Biotechnological advances in goat reproduction. Animal Reproduction Science. 75: 578- 585.
Beccaglia, M., Anastasi, P., Chigioni, S. and Luvoni, G. (2009). Tris-Lecithin Extender Supplemented With Antioxidant Catalase for Chilling of Canine Semen. Reproduction in Domestic Animals. 44: 345-349.
Bucak, M.N., Atessahin, A., Varıslı, O., Yuce, A., Tekin, N. and Akcay, A. (2007). The influence of trehalose, taurine, cysteamine andhyaluronan on ram semenMicroscopic and oxidative stress parameters afterfreeze–thawing process.Theriogenology. 67:1060–1067.
Bucak, M.N., Sarıözkan, S., Tuncer, P.B., Sakinand, F. and Kulaksız, R. (2010). The effect of antioxidants on post-thawed Angora goat (Capra hircusancryrensis) sperm parameters, lipid peroxidation and antioxidant activities. Small Ruminant Research. 89: 24-30.
Bucak, M.N., Tuncer, P.B., Sarıözkan, S., Ulutas, P.A., Çoyan, K. and Baspınar, N. (2009). Effects of hypotaurine, cysteamine and aminoacids solution on post-thawmicroscopic and oxidative stress parameters of Angora goat semen. Research in Veterinary Science. 87: 468–472.
Curry, M.R. (2000). Cryopreservation of semen from domestic livestock. Reviews of Reproduction. 5: 46-52.
Del Valle, I., Gomez-Duran, A., Holt, W., Muino-Blanco, T. and Cebrian-Perez, J. (2012). Soy Lecithin Interferes With Mitochondrial Function in Frozen-Thawed Ram Spermatozoa. Journal of Andrology.33: 717-725.
Esterbauer, H. and Cheeseman, K.H. (1990). Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods in enzymology. 186: 407-421.
Evans, G. and Maxwell, W.M.C. (1987). Handling and examination semen. In:Maxwell WMC, editor. Salamon’s artificial insemination of sheep and goat. Sydney: Butterworths. PP: 93–106.
Forouzanfar, M., Sharafi, M., Hosseini, S., Ostadhosseini, S., Nili N., Rahmani, H. and Nasr-Esfahani, M. (2010). In vitro comparison of egg yolk–based and soybean lecithin–based extenders for cryopreservation of ram semen. Theriogenology. 73: 480-487.
Graham, J. and Foote, R. (1987). Effect of several lipids, fatty acyl chain length, and degree of unsaturation on the motility of bull spermatozoa after cold shock and freezing. Cryobiology. 24: 42-52.
Holt, W., and North, R. 1994. Effects of temperature and restoration of osmotic equilibrium during thawing on the induction of plasma membrane damage in cryopreserved ram spermatozoa. Biology of Reproduction. 51: 414-424.
Holt, W.V. (2000). Basic aspects of frozen storage of semen. Animal Reproduction Science. 62: 3-22.
Ijaz, A., Hussain, A., Aleem, M., Yousaf, M.S. and Rehman, H. (2009). Butylated hydroxytoluene inclusion in semen extender improves the post-thawed semen quality of Nili-ravi buffalo. Theriogenology. 71:1326-1329.
Leboeuf, B., Restall, B. and Salamon, S. (2000). Production and storage of goat semen for artificial insemination. Animal Reproduction Science. 62: 113-141.
Maxwell, W.M. and Salamon, S. (1993). Liquid storage of ram semen: a review. Reproduction and Fertility Development. 5: 613–638.
Moussa, M., Martinet, V., Trimeche, A., Tainturier, D. and Anton, M. (2002). Low density lipoproteins extracted Theriogenology. 57: 1695-1706.
Parks, J. and Graham, J. (1992). Effects of cryopreservation procedures on sperm membranes. Theriogenology. 38: 209-222.
Pérez-Garnelo, S,. Oter, M., Borque, C., Talavera, C. and De la Fuente, J. (2006). Post-thaw viability of European Bison (Bison Bonasus) semen frozen with extenders containing egg yolk or lipids of plant origin and examined with a heterologous in vitro fertilization assay. Journal of Zoo and Wildlife Medicine. 37: 116-125.
Perez, L.J., Valcarcel, A., Heras, M.A., Moses, D.F. and Baldassarre, H. (1996). In vitro capacitation and induction of acrosomal exocytosis in ram spermatozoa as assessed by the chlortetracycline assay. Theriogenology. 45: 1037–1046.
Purdi, P.H. (2006) . A review on goat cryopreservation. Small Ruminant Research. 63: 215-225.
Quinn, P., Chow, P. and White, I. (1980). Evidence that phospholipid protects ram spermatozoa from cold shock at a plasma membrane site. Journal of Reproduction and Fertility. 60: 403-407.
Revell, S.G. and Mrode, R.A. (1994). An osmotic resistance test for bovine semen. Animal Reproduction Science. 36: 77–86.
Salamon, S. and Maxwell, W. (2000). Storage of ram semen. Animal Reproduction Science. 62: 77-111.
Schafer, S. and Holzmann, A. (2000). The use of transmigration and spermac stain toevaluate epididymal cat spermatozoa. Animal Reproduction Science. 59: 201–211.
Trimeche, A., Anton, M., Renard, P., Gandemer, G. and Tainturier, D. (1997). Quail egg yolk: a novel cryoprotectant for the freeze preservation of Poitou jackass sperm. Cryobiology. 34: 385-393.
Vick, M., Bateman, H., Lambo, C. and Swanson, W. (2012). Improved cryopreservation of domestic cat sperm in a chemically defined medium. Theriogenology. 78: 2120-2128.
Watson, P. (1976). The protection of ram and bull spermatozoa by the low density lipoprotein fraction of egg yolk during storage at 5 C and deep-freezing. Journal of Thermal Biology. 1: 137-141. :"B Zar";mso-ansi-language:EN-US;mso-fareast-language:EN-US; mso-bidi-language:FA'>. 87:1519-1527.
)