Document Type : Research Paper

Authors

1 1Department of Animal Science, Faculty of Agriculture, University of Tabriz, Iran

2 Department of Animal Science, Faculty of Agriculture, University of Tabriz, Iran

3 Member of Scientific Board

4 Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Iran

5 Animal Science Research Institute of Iran

Abstract

Nowadays, Incorporation of both phenotypes and genotype information enhanced the accuracy of genetic parameter predictions for growth characteristics in breeding schematics. The purpose of this study was to identify quantitative traits controlling positions (QTLs) associated with live body weight traits and growth characteristics using specific microsatellite markers in the 605 cm distance of goat chromosomes. In this regards, 4 half sib families were selected from large herd pedigree including 112 individual. Investigated records were included: birth, weaning, 6 months weights, average daily gain, calculated Keliber ratio index for two measured periods of birth- weaning age, weaning age- 6 months age. In the molecular study phase, a total of 45 microsatellite markers belong to 5 chromosome numbers (1, 2, 5, 6, and 26) with a pre-condition of the heterozygote pattern for microsatellite marker in each sire genotypes in progenies of heterozygote sires. The key findings of the present study are the identification of two QTLs between BMC1009-RM029 and INRABEN172 markers that are related to birth weight in chromosomes 5 and 26 (p<0.01). Also, after a across familial analysis, a QTL close to the BM1312 marker was detected in relation to the KBR1 ratio on chromosome 1(p<0.05).

Keywords

ساقی، د.ع.، اسلمی نژاد، ع.‌ا.، طهمورث‌پور، م.، نصیری، م.ر و داشاب، غ.ر. (1391).  مکان یابی جایگاه‌های صفت کمّی (QTL) موثر بر وزن بدن در بخشی از ژنوم گوسفند بلوچی.  نشریه علوم دامی (پژوهش و سازندگی). دوره 25، صفحه 57 – 49.
Beckman, J. S., and M. Soller. )1983(. Restriction fragment length polymorphisms in genetic improvement-methodologies, mapping and costs. Theoretical  Applied Genetics. 67:35–43
Casas, E., Shackelford, S.D., Keele, J. W., Koohmaraie, M., Smith, T. P. L. and Stone, R.T. (2003). Detection of quantitative trait loci for growth and carcass composition in cattle. Journal of Animal Science. 81:2976-83. 
Churchill, G. A. and Doerge, R.W. (1994). Empirical threshold values for quantitative trait mapping. Genetics, 138:963–971.
Dekkers, J.C. M. (2004). Commercial application of marker- and gene assisted selection in livestock: strategies and lessons. Journal Animal  Science. 82 (E Suppl.), E313–328.
Dodds, K.G., McEwan, J.C. & Davis, G.H.(2007). Integration of molecular and quantitative information in sheep and goat industry breeding program. Small Ruminant Research. 70, 32-41.
Ellegren, H. (2004). Microsatellites: Simple sequences with complex evolution. National Review Genetics 5(6): 435-45.
Esmailizadeh, K. A., Mohammad Abadi, M R., Asadi Foozi, M.( 2008). Mapping quantitative trait loci in livestock using simple linear regression. Iranian Journal Animal Science. 39, 83–93.
Ezmailizadeh, A.K.(2010). A partial genome scan to identify quantitative trait loci affecting birth weight in Kermani sheep. Small Ruminant Research. 94, 73–78.
FAO. (2007) Status of animal genetic resources. In: The state of the world’s animal genetic resources for food and agriculture (Ed. by D. Pilling & B. Rischkowsky), pp. 23–49. FAO, Rome.
Goldstein, D. B. and Pollock, D. D. (1997). Launching microsatellites: A review of mutation processes and methods of phylogenetic inference. Journal of Heredity 88: 335-342.
Green, P., Falls, K. and Crooks, S. (1990). Documentation for CRI-MAP, version 2.4. Washington University School of Medicine, St. Louis.
Kleiber, M. (1947). Body size and metabolic rate. Physiology Review. 27:511–541.
Knott, S.A., Elsen, J.M. and Haley, C. S. (1996) Methods for multiple marker mapping of quantitative trait loci in half-sib populations. Theoretical and Applied Genetics 93, 71–80.
Lander, E. and Kruglyak, L. (1995). Genetic dissection of complex traits: guidelines for interpreting and reporting linkage results. Nature Genetics, 11: 241–247.
Lander, E. S. and Botstein, D. (1989). Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics, 121: 185-199.
Maddox, J. and Cockett, N.(2007). An update on sheep and goat linkage maps and other genomic resources. Small Ruminant Research. 70, 4–20.
Menezes,W.H.Sous,E.P.Cavalcanti-Filho,L.T.Gama.(2016).Genetic parameters for reproduction and growth traits in Boer goatsin Brazil.Small Ruminant Research136 (2016) 247–256
Mohammedabadi, M. R., Askari N., Baghizadeh A. and Esmailizadeh, A. K. (2009) A directed search around caprine candidate loci provided evidence for microsatellites linkage to growth and cashmere yield in Rayini goats. Small Ruminant Research81,146–51.
Raadsma, H., Thomson, P., Zenger, K., Cavanagh, C., Lam,M., Jonas, E., Jones,M., Attard, G., Palmer, D. and Nicholas, F.(2009). Mapping quantitative trait loci (QTL) in sheep. I. A new male framework linkage map and QTL for growth rate and body weight. Genetic Selection and Evaluation. 41, 34.
Renaville, R., Gengler, N., Vrech, E., Prandi, A., Massart, S., Corradini, C., Bertozzi, C., F. Mortiaux, Burny, A. and Portetelle, D. (1997). Pit-1 gene polymorphism, milk yield, and conformation traits for Italian Holstein-Friesian bulls. Journal of Dairy Science. 80, 3431–3438.
SAS. (2009). SAS, SAS/sat user's guide, release 9.1. SAS Institute Inc., Cary, NC, USA
Vaiman 1996
Vaiman, D., Schibler, L., Bourgeois, F., Oustry, A., Amigues, Y.  and Cribiu E. P. (1996) A genetic linkage map of the male goat genome. Genetics 144, 279–305.
Van derWerf, J. H.J., Marshall, K., . and Sanghong, L.(2007). Methods and experimental designs for detection of QTL in sheep and goats. Small Ruminant Research. 70, 21–31.
Visser, C., Van Marle-Kster, E., Snyman, M.A., Bovenhuis, H. and Crooijmans, R..P.M.A. (2013) Quantitative trait loci associated with pre-weaning growth in South African Angora goats. Small Ruminant Research112, 15–20.
Walling, G. A., Visscher, P. M.,  Wilson, A. D.,  McTeir, B. L., Simm, G. and Bishop, S. C. (2004). Mapping of quantitative trait loci for growth and carcass traits in commercial sheep populations. Journal of Animal Science. 82:2234–2245.
Weller, J. I.(2009). Quantitative Trait Loci Analysis in Animals. CABI Publishing, London, UK;
Yeh, F. C., Boyle, T. and Yang, R. (1999). Popgene version 1.31. Microsoft window based freeware for population genetic analysis. University of Alberta Canada.