Document Type : Research Paper

Authors

1 Sari Agricultural Science and Natural Resources University

2 Sari Agricultural Sciences and Natural Resources University

Abstract

In order to evaluate the effect of diets with different levels of energy and protein on ruminal pH, ammonia nitrogen, nutrient digestibility and excretion of purine derivatives, an experiment was designed. Six experimental treatments were allocate in six fitulated seep in repeated measure desind. Treatments included: Control; treatment with high level of energy using barley grain; treatment with high energy level using barley grain and corrected by peptide, treatment with high level of energy using corn grain; treatment with high energy level using corn grain and corrected by peptide and treatment with high level of protein with increasing levels of soybean meal. Results showed experimental treatments were significant effect on pH and ammonia nitrogen released in the rumen, in three hours after feeding (p <0.05). Dry matter intake, fat and organic matter digestibility were not affected by experimental treatments (p >0.05). Control and high protein levels treatments significantly less intake of crude protein, Nutral Detergent Fiber and Acid Detergent Fiber (p <0.05). The digestibility of nutrients has improved with addition of energy and protein sources. The purine derivatives excreation were significantly higher in treatment with high protein levels than other groups (p <0.05). Supplementation of diets with carbohydrate and protein sources with the least adverse effects on gastrointestinal is recommended.

Keywords

افشار، س.، کاظمی بن چناری، م. و فردوسی، ح. (1394). تأثیر تغذیه دانه جو کامل و پرک شده همراه با دو منبع پروتئین کنجاله سویا و اوره بر قابلیت هضم مواد مغذی و فراسنجه های شکمبه‌ای در گوسفند نژاد مهربان. پژوهش‌های تولیدات دامی. 11. 102-107.
پاپی، ن.، کوهستانی م.، مقصودی نژاد، ق.، اکبری، ا. و ارفعی، م. (1393). اثرات جایگزینی دانه جو با ذرت بر عملکرد پروار بره‌های نر. تحقیقات کاربردی در علوم دامی. 12؛ 33-42.
نجفی، ش.، طباطبایی، م. م.، زابلی، خ.، احمدی، ا. و ساکی،  ع ا. (1396).اثر متقابل بین فرآوری دانه جو و نوع منبع نیتروژن جیره غذایی بر قابلیت هضم، متابولیسم نیتروژن و تولید پروتئین میکروبی در گوسفند مهربان. تحقیقات تولیدات دامی. (6) 1: 39-51.
یوسفیان، س.، تیموری یانسری، ا. و انصاری، ز. (1389). بررسی اثرات کنجاله سویای تیمار شده با پرتو ماکروویو و متیونین محافظت‌شده بر عملکرد بره‌های پرواری. پایان‌نامه کارشناسی ارشد دانشگاه علوم کشاورزی و منابع طبیعی ساری.
یوسفیان، س.، تیموری یانسری، ا. و چاشنی دل، ی. (1395). اثر اندازه ذرات و الیاف نامحلول در شوینده خنثی غیر قابل هضم جیره بر عملکرد بره های پرواری. نشریه پژوهش در نشخوارکنندگان. (4) 3: 135-156
 AOAC. (1990). Official methods of Analysis. 15th ed. Association of official analytical chemists, Arlington, VA.
Atkinson, R.L., Toone,  C.D and Ludden, P.A. (2010). Effects of ruminal protein degradability and frequency of supplementation on site and extent of digestion and ruminal fermentation characteristics in lambs fed low-quality forage. Journal of  Animal  Science. 88: 718–726.
Brito,  A.F and Broderick,  G.A. (2007). Effects of different protein supplements on milk production and nutrient utilization in lactating dairy cows, Journal of  Dairy Science. 90: 1816-1827.
Cannas, A., Tedeschi, L.O., Fox, D.G., Pell, A.N., and Van Soest, P.J. (2004). A mechanistic model to predict nutrient requirements and feed biological values for sheep. J. Anim. Sci. 82: 149-169.
Chen,  X.B. and Gomes,  M.J. (1992). Estimation of microbial protein supply to sheep and cattle based on urinary excretion of purine derivatives. An overview of technical detail. International.feed resources units, Rowett Research Institute. Occasional publication, Aberdeen.
Cole, N.A. and Todd, R.W. (2008). Opportunities to enhance performance and efficiency through nutrient synchrony in concentrated fed animals, Journal of Animal Science. 86: 318-333.
Davies, K. L., McKinnon, J. J. and Mutsvangwa, T. (2013). Effects of dietary ruminally degradable starch and ruminally degradable protein levels on urea recycling, microbial protein production, nitrogen balance, and duodenal nutrient flow in beef heifers fed low crude protein diets. Canadian Journal of Animal Science, 93:123-136.
Feng, P., Hunt, C.W., Pritchard, G.T. and Parish, S.N. (1995). Effect of barley variety and dietary barley content on digestive function in beef steers fed grass hay-based diets. Journal of Animal  Science. 73: 3476–3484.
Flis,  S.A. and Wattiaux,  M.A. (2005). Effects of parity and supply of rumen degraded and undegraded protein on production and nitrogen balance in Holsteins. Journal of Dairy Science. 88: 2096-2106.
Griswold,  K. E., Apgar,  G. A., Bouton,  J. and Firkins,  J. L. (2003). Effects of urea infusion and ruminal degradable protein concentration on microbial growth, digestibility, and fermentation in continuous culture, Journal of Animal Science, 81: 329-336.
Haddad, S. G., and R. E. Naser. (2007). Partial  replacement of barley grain for corn grain: Associative effects on lambs’ growth performance. Small Ruminant Research. 72: 92-95.
Jabbar,  M.A., Marghazani,  I.B., Pasha, T.N., Khalique, A. and Abdullah,  M. (2013). Effect of protein supplements of varying ruminal degradability on nutrient intakes, digestibility, nitrogen balance and body condition score in early lactating Nili-Ravi Buffaloes. Journal of Animal  Plant  Science. 23: 108-112.
jooste,  A.M. (2012). Effect of diets differing in rumen soluble nitrogen on poor quality roughage utilization by sheep. M.Sc. thesis. University of Pretorioa, South Africa.
Kargar, S., Ghorbani, G.R., Alikhani, M., Khorvash, M., Rashidi, L and Schingoethe, D.J. (2012). Lactational performance and milk fatty acid profile of Holstein cows in response to dietary fat supplements and forage: concentrate ratio. Livest. Sci. 150: 274–283.
Khalesizadeh,  A., Vakili, A. R., Danesh Mesgaran,  M. and Valizadeh, R. (2011). The effects of garlic oil (Allium sativa), turmeric powder (Curcuma iongalinn) and monensin on total apparent digestibility of nutrients in Baloochi lambs. International Journal of Biological, Bimolecular, Agricultural, Food and Biotechnological Engineering, 5(11):791-793.
Khalid, M. F., Sarwar, M., Rehman, A. U., Shahzad, M. A. and Mukhtar, N. (2012). Effect of Dietary Protein Sources on Lamb’s Performance: A Review. Iranian Journal of Applied Animal Science 2(2): 111-120.
Kiran D. and Mutsvangwa T. (2007). Effects of barley grain processing and dietary ruminally degradable protein on urea nitrogen recycling and nitrogen metabolism in growing lambs. Journal of Animal Science, 85:3391-3399.
Kyriazakis, I. and Oldham,  J.D. (1997). Food intake and diet selection in sheep: The effect of
manipulating the rate of digestion of carbohydrates and protein of the feeds offered as a choice, British Journal of Nutrition. 77: 243-254.
Maekawa, M., Beauchemin,  K.A. and Christensen, D.A. (2002). Effect of concentrate level and feeding management on chewing activities, saliva production and ruminal pH of lactating dairy cows. Journal of Dairy Science. 85: 1165-1175.
Matras, J., Bartle,  S.J. and Preston, R.L. (1991). Nitrogen utilization in growing lambs: effects of grain (starch) and protein source with various rate of ruminal degradation, Journal of Animal Science. 69: 339- 347.
Mc Carthy,  R.D., Klusmeyer, T.H., Vicni, J.L. and Clack, J.H. (1989). Effect of source of protein and carbohydrate on ruminal fermention and passage of nutrients to the small intestine of lactating cows, Journal of  Dairy Science. 72: 2002-2009.
McAllister, T. A., Cheng, K.J., Rode, L. M. and Forsberg, C. W. (1990). Digestion of barley, maize, and wheat by selected species of ruminal bacteria. Applied and Environmental Microbiology , 56 (10), 3146-3153.
Moore,  K.P. (2000). Laboratory procedures for the plant tissue and feed analysis laboratory. Agricultural services laboratory, Clemson University. Clemson. S.C.
Nikkhah, A. and Amanlou, V. (1990). The importance of dietary protein for ruminants and its use in diets. Zanjan University Press. 223p.
NRC. (Ed.). (2007). Nutrient requirements of small ruminants: sheep, goats, cervids, and New World camelids. The National Academies Press Washington, DC
Pond, W.G., Church, D.C., Pond, K.R. and Schoknecht, P.A. (2005). Basic animal nutrition and feeding. 5th edition
Reynal, S.M., Ipharraguerre,  I. R., Lin˜eiro,  M., Brito,  A. F., Broderick,  G. A and Clark, J.H. (2007). Omasal flow of soluble proteins, peptides, and free amino acids in dairy cows fed diets supplemented with proteins of varying ruminal degradabilities, Journal of  Dairy Science. 90: 1887-1903.
Ribeiro,  S.S., Vasconcelos, J.T., Morais,  M.G., Itavo,  C.B.C.F and Franco,  G.L. (2011). Effects of ruminal infusion of a slow-release polymer-coated urea or conventional urea on apparent nutrient digestibility, in situ degradability, and rumen parameters in cattle fed low quality hay. Animal Feed Science and Technology. 164: 53–61.
Russell, J.B., O’Conner, J.D., Fox, D.G., Van Soest,  P.J. and Sniffen, C.J. (1992). A net carbohydrate and protein system for evaluating cattle diets: I. Ruminal fermentation, Journal of Animal Science. 70:3551-3561.
Santos,  F.A., Santos, J.E., Theurer, C.B. and Huber, J.T. (1998). Effects of rumen undegradable protein on dairy cow performance: a 12-year literature review. Journal of Dairy Science. 81: 3182-213.
Sinclair L. A., Garnsworthy P. C., Newbold J. R. and Buttery P. J. 1993. Effect of synchronizing the rate of dietary energy and nitrogen release on rumen fermentation and microbial protein synthesis in sheep. Journal of Agricultural Science, 120:251-263.
Small Ruminant Nutrition System. Version, 1.9.5566.
Souza, N.K.P., Detmann, E., Valadares Filho, S.C., Costa, V.A.C., Pina, D.S., Gomes, D.I., Queiroz, A.C and Mantovani, H.C. (2013). Accuracy of the estimates of ammonia concentration in rumen fluid using different analytical methods. Arq. Bras. Med. Vet. Zootec. 65: 1752-1758
Van Soest, P.J., Roberts, J.B. and Lewis, B.A. (1991). Methods of dietary fiber, neutral detergent fiber and noN.S.trach polysaccharides in relation to animal nutrition, Journal of Dairy Science. 74: 3583- 3597.
Zhao, X.G., Wang, M., Tan, Z.L., Tang, S.X., Sun, Z.H., Zhou, C.S., and Han, X.F. (2009). Effects of rice straw particle size on chewing activity, feed intake, rumen fermentation and digestion in goats. Asian-Aust. J. Anim Sci. 22: 1256 – 1266.