The effect of in ovo feeding of DL-methionine on small intestine morphology in a day-old broiler chicks

Document Type : Research Paper

Authors

1 Assistant Professor, Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.

2 MSc student in Animal Physiology, Department of Animal Science, University of Tabriz, Iran.

3 Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

4 Professor at Department of Histology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

5 Professor, Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.

6 Associate Professor, Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

7 Dep of Animal Science, University of Tabriz, Tabriz, Iran

Abstract

In ovo feeding is considered as a useful tool to overcome the growth limitations during embryonic growth and after hatching in domesticated poultry. Therefore, the purpose of the present study was to investigate the effect of different levels of DL- methionine on the morphology of small intestine in ROSS 308 broiler chicks. In this regard, the total number of 240 eggs from Ross 308 broiler breeder were used according to a completely randomized design (CRD) with 8 experimental groups and 30 eggs per each treatment. The experimental treatments included different levels of DL-methionine amino acid (0.19, 0.38, 0.57, 0.76, 0.95 and 1.14 %), along with a sham-control group (sterile water injection) and a control group (without injection) which were injected into the amniotic fluid on the 14th d of incubation. After hatching, the chicks were weighed and slaughtered to measure small intestine morphological characteristics. Results of the study indicated that in ovo feeding of DL-methionine had a significant effect (p<0.05) on hatchability, weight and relative weight of jejunum, small intestine weight, as well as crypt diameter (except for duodenum), villus height, crypt depth, villus height to crypt depth ratio, and villus thickness in duodenum, jejunum, and ileum. On the other hand, no effect of treatments (p>0.05) was observed on chick weight and weight and length indexes of duodenum and ileum. Based on the results of this study, the best morphological response was observed in 76% DL-methionine treatment.

Keywords

References

پوررضا، ج. (1388). اصول علمی و عملی پرورش طیور، انتشارات جهاد دانشگاهی واحد صنعتی اصفهان (تالیف).
پوستی، ا. و ادیب مرادی، م. (1387). روش‌های آزمایشگاهی بافت شناسی.انتشارات دانشگاه تهران. صفحه 16-22.
تهامی، ز.، حسینی، س. م. و باشتنی، م. (1393). اثر مکمل اسیدهای آلی بر برخی خصوصیات دستگاه گوارش و مورفولوژی روده باریک جوجه‌های گوشتی. تحقیقات تولیدات دامی. سال سوم، شماره سوم، ص ص 10-1.
صلاحی، 1. (1394). راهنمای کامل جوجه‌کشی. انتشارات آییژ.
قوچخانی، ر. 1395. اثر تزریق درون تخم مرغی نسبت‌های مختلف دی‌-ال متیونین به ال-لایزین بر شاخص‌های لاشه، غلظت متابولیت‌های خونی، مورفولوژی بافت روده و سیستم ایمنی در جوجه‌های گوشتی یک روزه. پایان نامه کارشناسی ارشد. دانشگاه تبریز.
محمدرضایی، ح. (1393). بررسی اثر متیونین بر خصوصیات هیستومورفومتری روده ی باریک جنین مرغ. پایان نامه دکتری حرفه‌ای، دانشگاه شهید باهنر کرمان.
نیکوفرد، و. (1390). اثرات متقابل سطح متیونین و تعادل الکترولیتی جیره بر عملکرد و پاسخ-های ایمنی جوجه‌های گوشتی. پایان نامه کارشناسی ارشد- دانشگاه صنعتی اصفهان.
ادیب مرادی م، ابراهیمی م، زارع شحنه ا، شیوازاد م، انصاری پیرسرایی ز، تبیانیان م و نوری جلیانی ک،1393. تأثیر ال-آرژنین بر رشد، رودە کوچک و سامانه ایمنی در جوجه‌های گوشتی در دورۀ آغازین پرورش. علوم دامی ایران، دوره 45، شماره 3، صفحه: 233-223.
متقی‌طلب م، شفیعی‌منش ی، 1394. اثر تزریق داخل تخم مرغی پیریدوکسین، متیونین و ترکیب آنها به تخم مرغ های مادر گوشتی بر میزان جوجه درآوری، وزن جوجه یکروزه و صفات تولیدی.تحقیقات تولیدات دامی، دوره 4، شماره 3، صفحه 57-66.
Bartell, S. M., and Batal, A. B. (2007). The effect of supplemental glutamine on growth performance, development of the gastrointestinal tract, and humoral immune response of broilers. Poultry Science, 86 (9): 1940-1947.
Bhanja, S.K. and Mandal, A.B. (2005). Effect of in ovo injection of critical amino acids on pre and post hatch growth, immunocompetence and development of digestive organs in broiler chickens. Asian-Australasian Journal of Animal Science, 18: 524-531.
Calder, P.C. and Yaqoob, P. (1999). Glutamine and the immune system. Amino Acids, 17: 227-241.
Cant, P.J., McBride, B.W. and Croom, Jr.W.J. (1996). The regulation of intestinal metabolism and its impact on whole animal energetics. Journal of Animal Science, 74: 2541-2553.
Chen, W., Wang, R., Wan, H.F., Xiong, X.L., Peng, P. and Peng, J. (2009). Influence of in ovo injection of glutamine and carbohydrates on digestive organs and pectoralis muscle mass in the duck. British Poultry Science, 50 (4): 436-442.‏
Coşkun, İ., Erener, G., Şahin, A., Karadavut, U., Altop A., and Ağma Okur, A. (2014). Impacts of in ovo feeding of DL-methionine on hatchability and chick weight. Turkish Journal of Agriculture - Food Science and Technology, 2(1): 47-50.
Ebrahimi, M., Janmohammadi, H., Daghigh Kia, H., Moghaddam, G., Rajabi, Z., Rafat, S.A., and Javanmard, A. (2017). The effect of L-lysine in ovo feeding on body weight characteristics and small intestine morphology in a day-old Ross broiler chicks. Revue de Médecine Vétérinaire, 168: 116-124.
Dilger, R.N. and Baker, D.H. (2007). DL-Methionine is as efficacious as L-methionine, but modest L-cystine excesses are anorexigenic in sulfur amino acid-deficient purified and practical-type diets fed to chicks. Poultry Science, 86:2367–2374.
Finkelstein, J.D. (1990). Methionine metabolism in mammals. The Journal of Nutritional Biochemistry, 1: 228–237.
Foye, O.T. (2005). The biochemical and molecular effects of amniotic nutrient administration, “in ovo feeding” on intestinal development and function and carbohydrate metabolism in turkey embryos and poults. Ph.D. Thesis, North Carolina State University, United States.
Foye, O.T., Ferket, P.R. and Uni, Z. (2005a). The effects of in ovo feeding of arginine and/or beta-hydroxy-beta-metylbutyrate (HMB) on glycogen metabolism and growth in turkey poults. Poultry Science, 84 (Supplement 1): 9.
Foye, O.T., Ferket, P.R. and Uni, Z. (2005b). The effects of in ovo feeding of beta-hydroxy-beta-methylbutyrate (HMB) and arginine on jejunal expression and function in turkeys. Poultry Science, 84 (Supplement 1): 41.
Foye, O.T., Ferket, P.R. and Uni, Z. (2007). The effects of in ovo feeding arginine, β-hydroxy-β-methyl-butyrate, and protein on jejunal digestive and absorptive activity in embryonic and neonatal turkey poults. Poultry Science, 86 (11): 2343-2349.
Foye, O.T., Uni, Z. and Ferket, P.R. (2006). Effect of in ovo feeding egg white protein, β-hydroxy-β-methylbutyrate, and carbohydrates on glycogen status and neonatal growth of turkeys. Poultry Science, 85: 1185-1192.
Geyra, A., Uni, Z. and Sklan, D. (2001). The effect of fasting at different ages on growth and tissue dynamics in the small intestine of the young chick. British Journal of Nutrition, 86 (01): 53-61.‏
Kadam, M. M., Barekatain, M. R., K Bhanja, S., & Iji, P. A. (2013). Prospects of in ovo feeding and nutrient supplementation for poultry: the science and commercial applications—a review. Journal of the Science of Food and Agriculture, 93 (15), 3654-3661.‏
Khajali, F. and Widerman, R.F. (2010). Dietary arginine: metabolic, environmental, immunological and physiological interrelationships. World's Poultry Science Journal, 66: 751-766.
Ojano-Dirain, C.P. and Waldroup, P.W. (2002). Evaluation of lysine, methionine and threonine needs of broilers three to six week of age under moderate temperature stress. International Journal of Poultry Science, 1: 16-21.
Pluske, J.R., Hampson, D.J. and Williams, I.H. (1997). Factors influencing the structure and function of the small intestine in the weaned pig- a review. Livestock Production Science, 51: 215-236.
SAS Institute Inc. (2008). SAS/STAT User's Guide, Version 9.2. Cary, NC: SAS Institute Inc.
Shen, Y.B., Ferket, P., Park, I., Malheiros, R.D. and Kim, S.W. (2015). Effects of feed grade-methionine on intestinal redox status, intestinal development, and growth performance of young chickens compared with conventional-methionine. Journal of Animal Science, 93 (6): 2977-2986.
Teshfam, M., Nodeh, H. and Hassanzadeh, M. (2005). Alterations in the intestinal mucosal structure following oral administration of triiodothyronine (T3) in broiler chickens. Journal of Applied Animal Research, 27 (2): 105-108.
Uni, Z. and Ferket, R.P. (2004). Methods for early nutrition and their potential. World's Poultry Science Journal, 60 (01): 101-111.
Uni, Z., Tako, E., Gal-Garber, O. and Sklan, D. (2003). Morphological, molecular, and functional changes in the chicken small intestine of the late-term embryo. Poultry Science, 82 (11): 1747-1754.
Widerman, J.R.F. and Bottje, W.G. (2000). In ovouse of L-arginine and salts thereof in the prevention and/or treatment of pulmonary hypertension syndrome in avians. United States patent. Patent NO. 612742.
Xu, G., Kwon, J., Marshall, C.A., Lin, T.A., Lawrence, J.C. and McDaniel, M.L. (1998). Branched-chain amino acids are essential in the regulation of PHAS-I and p70 S6 kinase by pancreatic β-cells. Journal of Biological Chemistry, 273: 28178-28184.
Animal Sciences Journal
Volume 32, Issue 122
June 2019
Pages 121-130

Files

Share

How to cite

Statistics