Research Journal of Livestock Science

Current Issue

By Issue

By Author

By Subject

Author Index

Keyword Index

About Journal

Aims and Scope

Editorial Board

Publication Ethics

Indexing and Abstracting

Related Links

FAQ

Peer Review Process

Journal Metrics

News

Phylogenetic relationship study of Iranian subspecies honeybee with other honeybee subspecies using morphological and molecular markers

Document Type : Research Paper

Authors

1 Razi university, Kermanshah, Iran

2 Razi university, KARMANSHAH, Iran

3 Razi university, KARMANSHAH, IRAN

Abstract

Morphological and molecular studies are considered as a powerful tool for estimating genetic diversity and the determination of phylogenetic relationships among different populations of honeybee subspecies. In the present study, morphological and molecular markers (PCR-RFLP) were used to study the phylogenetic relationships of Iranian subspecies honeybee with other honeybee subspecies around the world. Samples were collected from 100 cities belonging to 20 Iranian provinces during the summer of 2016. A total of 2,250 and 300 worker bees were studied for morphological and molecular analyses, respectively. The results of phylogenetic trees plotted using morphological and molecular markers revealed that 29 honeybee subspecies were classified into five groups. In this clustering, the Iranian subspecies honeybee (A. m. meda) with A. m. cyprica, A. m. syriaca, A. m. anatolica, A. m. armeniaca, A. m. caucasica, A. m. caucasica, Am. Pomonella subspecies were assigned in same cluster. This group included subspecies from Eastern Mediterranean, the Near East and the East of the Middle East (O), which was reported in previous studies. The results showed that the honeybee subspecies (or race) in Iran was exactly the same as the Iranian honeybee subspecies (A. m. meda); it also seems that imports of foreign subspecies in the past two decades and the trafficking imports of queen in the last decade had no significant impact on Iranian honeybee subspecies genetic purity due to its adaptation to the country's climates and the instability and incompatibility of other imported subspecies, so that it has not lost its genetic identity.

Keywords

References
عبادی، ر.، احمدی، ع.ا. 1387. پرورش زنبورعسل. انتشارات چاپخانه راه نجات، اصفهان، ایران. ص 16، 17 و 18.
رحیمی، ع. 1295. بررسی تنوع ژنتیکی و روابط فیلیوژنتیکی زنبورعسل زیرگون ایرانی با استفاده از نشانگرهای ریخت شناسی و مولکولی. پایان نام دکترای تخحصصی رشته حشره شناشی، دانشگاه رازی.
طهماسبی، غ. 1375. مطالعه مورفولوژیکی و بیوشیمیایی توده­های زنبورعسل ایران . پایان نامه دکترای حشره شناسی کشاورزی، دانشگاه تربیت مدرس.
طهماسبی، غ.، عبادی، ر.، اسماعیلی، م. و کامبوزیا، ج. 1377. مطالعه مورفولوژیک زنبورعسل معمولی (Apis mellifera L.) در ایران. مجله علوم کشاورزی و منابع طبیعی، شماره 2، ص 89-100.
طهماسبی، ع.، سروستانی، م.، عبادی، ر.، جوارمی، ا.، بابایی، م.، بانه، ح.، قره­داغی، ع.، افروزان، ه.، جمشیدی، م. و سیفی، ع. 1395. تعیین پارامترهای ژنتیکی و فنوتیپی صفات تولید عسل، بچه­دهی و رفتار دفاعی در کلنی­های زنبورعسل نسل چهاردهم در استان­های مرکزی کشور. مجموعه مقالات اولین کنگره بین المللی و نهمین کنگره پژوهشی زنبورعسل ایران. موسسه تحقیقات علوم دامی کشور. ص 5.
Aljianabi, S.M. and Martinez, I. (1997). Universal and rapid salt extraction of high quality genomic DNA for PCR-based techniques. Nucleic Acids Research, 25: 4692-4693.
Arias, M.S. and Sheppard, W.S. (2005). Phylogenetic relationships of honey bees (Hymenoptera:Apinae:Apini) inferred from nuclear and mitochondrial DNA sequence data. Molecular Phylogenetics and Evolution, 37(1): 25–35.
Avise, J.C., Arnold, J. and Ball, R.M. (1987). Intraspecific phylogeography: the mitochondrial DNA bridge between population genetics and systematics. Annual Review of Ecology, Evolution, and Systematics, 18(2): 489-522.
Bouga, M., Harizanis, P.C., Kilias, G. and Alahiotis, S. (2005). Genetic divergence and phylogenetic relationships of honeybee Apis mellifera (Hymenoptera: Apidae) populations from Greece and Cyprus using PCR-RFLP analysis of three mtDNA segments. Apidologie, 36(1): 335-344.
Dupraw, E.J. (1965). The recognition and handling of honey bee specimens in non linnean taxonomy. Journal of Apicultural Research, 4(2): 71 – 84.
Engel, M.S. 1999. The taxonomy of recent and fossil honey bees (Hymenoptera: Apidae). Journal of Hymenoptera Research, 8(1): 165-196.
Franck, P., Garnery, L., Solignac, M. and Cornuet, J.M. (2000). Molecular confirmation of a fourth lineage in honeybees from the Near East. Apidologie, 3(1)1: 167–180.
Garnery, L., Solignac, M., Celebrano, G. and Cornuet, J.M. (1993). A simple test using restricted PCR-amplified mitochondrial DNA to study the genetic structure of Apis mellifera L. Experientia, 49(2): 1016-1021.
Meixner, M.D., Leta, M.A., Koeniger, N. and Fuchs, S. (2011). The honey bees of Ethiopia represent a new subspecies of Apis mellifera – Apis mellifera simensis n. ssp. Apidologie, 42(2): 425-43.
Morse, R.A., Calderone, N.W. (2000). The Value of Honey Bees As Pollinators of U.S. Crops in 2000. Pollination, 1-15.
Özdil, F., Fakhri, B., Meydan, H., Yildiz, M.A. and Hall, H.I. (2009). Molecular characterization of Turkish honey bee population(Apis mellifera) inferred from mitochondrial DNA RFLP and sequence results. Apidologie, 40(5): 570-576.
Palmer, M., Smith, D. and Kaftano, A.O. (2000). Genetic variation and evidence for a fourth lineage of Apis mellifera mtDNA. Journal of Heredity, 4(2): 42- 46.
Papachristoforou, A., Rortais, A., Bouga, M., Arnol, G. and Garnery, L. (2013). Genetic characterization of the cyprian honey bee (Apis mellifera cypria ) based on microsatellites and mitochondrial DNA polymorphisms. Journal Apiculture Science, 57(2): 127-134.
Rahimi, A., Miromayedi, A., Kahrizi, D., Abdolshahi, R., Kazemi, E. and Yari, K. (2014). Microsatellite genetic diversity of Apis mellifera meda skorikov. Molecular Biology Reports, 41(3): 7755-7761.
Rahimi, A., Hasheminasab, H. and Azati, N. (2015). Predicting honey production based on morphological characteristics of honey bee (Apis mellifera L.) using multiple regression model. Ecology-Environment-Conservation, 21(1): 29-33.
Rahimi, A., Mirmoayedi, A., Kahrizi, D., Zarei, L, and Jamali, S. 2016. Genetic diversity of Iranian honey bee(Apis mellifera meda Skorikow, 1829) populations based on ISSR markers. Cellular and Molecular Biology, 62 (4): 53-58.
Rahimi, A., Mirmoayedi, A., Kahrizi, D., Zarei, L, and Jamali, S. (2017). Morphological diversity and phylogenetic relationships Study of Iranian subspecies honey bee (Apis mellifera meda) populations via morphological characteristics. Sociobiology,  64(1): 33-41.
Rahimi, A., Mirmoayedi, A., Kahrizi, D., Zarei, L, and Jamali, S. (2018). Genetic Variation in Iranian Honeybees, Apis mellifera meda Skorikow, 1829, (Hymenoptera: Apidae) Inferred from RFLP Analysis of two mtDNA Regions (COI and 16S rDNA). Sociobiology, In press.
Royan, M., Rahimi, G., Esmaeilkhanian, S. and Ansari, Z. (2007). A study on the genetic diversity of the Apis mellifera meda population in the south coast of the Caspian Sea using microsatellite markers. Journal of Apicultural Research and Bee World, 46(4): 236–241.
Ruttner, F., Tassencourt, L. and Louvaux, J. (1978). Biometrical – statistical analysis of the geographic variability of Apis mellifera L. Apidologie, 9 (4): 363 – 381.
Ruttner, F., Pourasghar, D. and Kauhausen, D. (1985). Honey bees of Iran .2. Apis mellifera meda Skorikoow. The Persian bee. Apidologie, 9(4): 363 – 381.
Ruttner, F. (1988). Biogeography and Taxonomy of Honeybees. Springer-Verlag. Berlin. 284 pp.
Ruttner, F. (1992). Naturgeschichte der Honigbienen. Ehrenwirth Verlag. Münich. Germany. 357pp.
Sheppard, W.S., Arias, M.C., Greech, A. and Meixner, M.D. (1997). Apis mellifera ruttneri, a new honey bee subspecies from Malta. Apidologie, 28(2): 287-293.
Sheppard, W.S. and Meixner, M.D. (2003). Apis mellifera pomonella, a new honey bee subspecies from Central Asia. Apidologie, 34(3): 367–375.
Research Journal of Livestock Science
Volume 32, Issue 123
September 2019
Pages 323-334
Files
Share
How to cite
Statistics