Document Type : Research Paper
Authors
1 Department of Animal Science, Faculty of Agriculture Science, University of Yasouj, Yasouj
2 Animal Science Department, Faculty of Agriculture Science, University of Yasouj,Yasouj, Iran
3 Department of Animal Science, Faculty of Agriculture, Yasouj University, Yasouj, Iran
Abstract
This study was conducted to investigate the effect of different levels of oak acorn and sex on abdominal fat and mRNA expression of pancreatic lipase gene in broiler chicken. A total of 264 one-day old boilers of male and female for Cobb 500 strain were assigned in a completely randomized design to 3 experimental groups each with 4 replicates (22 broilers per replication). In the first treatment (control), the broiler chicks were fed with early and end-of-corn diets (without oak), while in the other two treatments, the diets contained 15 or 20% oak acorn. In order to determine the effect of treatments on lipase gene expression at the age of 21 and 42 days from each treatment, 6 broilers were slaughtered and sampled from their pancreatic tissue. Gene expression data were analyzed using SAS and REST softwares. The results showed that the expression of the pancreatic lipase gene was significantly higher in 15 and 20% oak acorn treatments relative to control group (p < 0.05) on day 21,however, there was no significant difference in expression of lipase gene on day 42 (P>0.05). In addition, relative weight of abdominal fat was significantly increased in both treatments of oak acorn on day 42 (p < 0.05).
Keywords
Ahn, J.H. Liu, Q. Lee, C. Ahn, M.J. Yoo, H.S. Hwang, B.Y. and Lee, M.K. (2012). A new pancreatic lipase inhibitor from Broussonetia kanzinoki. Bioorganic & medicinal chemistry letters. 22(8):2760-2763.
Alcicek, A. Bozkurt, M. and Çabuk, M. (2004). The effect of a mixture of herbal essential oils, an organic acid or a probiotic on broiler performance. South African Journal of Animal Science. 34(4):217-222.
Basmacioglu Malayoglu, H. Baysal, S. Misirlioglu, Z. Polat, M. Yilmaz, H. and Turan, N. (2010). Effects of oregano essential oil with or without feed enzymes on growth performance, digestive enzyme, nutrient digestibility, lipid metabolism and immune response of broilers fed on wheat–soybean meal diets. British poultry science. 51(1):67-80.
Biswas, A.H. and Wakita, M. (2001). Effect of dietary Japanese green tea powder supplementation on feed utilization and carcass profiles in broilers. The Journal of Poultry Science. 38(1):50-57.
Bohannon-Stewart, A. Kelley, G. Kimathi, B. Subramanya, S.H.K. Donkor, J. Darris, C. Tyus, J. Payne, A. Byers, S. Hui, D. and Nahashon, S. (2014). Expression of potential regulatory genes in abdominal adipose tissue of broiler chickens during early development. Genetics research international, 2014.
Bojarpour, M. Bahmaninia, E. Ebrahimi, R. and Fayazi, J. (2010). Evaluate effects of different inclusion of oak kernel with determine food potential oak kernel substitute with corn seed on broiler chickens ration. Research Journal of Biological Sciences. 5:17-19.
Bouderoua, K. Mourot, J. and Selselet-Attou, G. (2009). The effect of green oak acorn (Quercus ilex) based diet on growth performance and meat fatty acid composition of broilers. Asian-Australasian Journal of Animal Science. 6:843-848.
Butterwith, S.C. (1997). Regulators of adipocyte precursor cells. Poultry science. 76(1):118-123.
Correia, P. Leitao, A. and Beirao-da-Costa, M.L. (2009). The effect of drying temperatures on morphological and chemical properties of dried chestnuts flours. Journal of Food Engineering. 90(3):325-332.
Crespo, N. and Esteve-Garcia, E. (2001). Dietary fatty acid profile modifies abdominal fat deposition in broiler chickens. Poultry science. 80(1):71-78.
Crowell, P.L. (1999). Prevention and therapy of cancer by dietary monoterpenes. The Journal of nutrition. 129(3):775-778.
Emmerson, D.A. (1997). Commercial approaches to genetic selection for growth and feed conversion in domestic poultry. Poultry Science. 76(8):1121-1125.
Fan, H.P. Xie, M. Wang, W.W. Hou, S.S. and Huang, W. (2008). Effects of dietary energy on growth performance and carcass quality of white growing Pekin ducks from two to six weeks of age. Poultry science. 87(6):1162-1164.
Fouad, A.M. and El-Senousey, H.K. (2014). Nutritional factors affecting abdominal fat deposition in poultry: a review. Asian-Australasian journal of animal sciences. 27(7):1057-1068.
Frazier, R.A. Deaville, E.R. Green, R.J. Stringano, E. Willoughby, I. Plant, J. and Mueller-Harvey, I. (2010). Interactions of tea tannins and condensed tannins with proteins. Journal of pharmaceutical and biomedical analysis. 51(2):490-495.
Griffiths, D.W. and Moseley, G. (1980). The effect of diets containing field beans of high or low polyphenolic content on the activity of digestive enzymes in the intestines of rats. Journal of the Science of Food and Agriculture. 31(3):255-259.
Huang, J. Zhang, Y. Zhou, Y. Zhang, Z. Xie, Z. Zhang, J. and Wan, X. (2013). Green tea polyphenols alleviate obesity in broiler chickens through the regulation of lipid-metabolism-related genes and transcription factor expression. Journal of agricultural and food chemistry. 61(36):8565-8572.
Hur, S.N. Molan, A.L. and Cha, J.O. (2005). Effects of feeding condensed tannin-containing plants on natural coccidian infection in goats. Asian- Australasian Journal of Animal Science. 18(9):1262-1266.
Jansman, A.J.M. (1993). Tannins in feedstuffs for simple-stomached animals. Nutrition research reviews. 6(1):209-236.
Kassim, H. and Suwanpradit, S. (1996). The effects of dietary energy levels on the carcass composition of the broilers. Asian-Australasian Journal of Animal Sciences. 9(3):331-335.
Khodadoust, S. Mohammadzadeh, A. Mohammadi, J. Irajie, C. and Ramezani, M. (2014). Identification and determination of the fatty acid composition of Quercus brantii growing in southwestern Iran by GC–MS. Natural product research. 28(8):573-576.
Khosravinia, H. Ghasemi, S. and Rafiei Alavi, E. (2103). Effect of savory (Satureja khuzistanica) essential oils on performance, liver and kidney functions in broiler chickens. Journal of Animal and Feed Sciences. 22(1):50-55.
Krogdahl, A. (1985). Digestion and absorption of lipids in poultry. The Journal of nutrition. 115(5):675-685.
Kumar, V. Elangovan, A.V. Mandal, A.B. Tyagi, P.K. Bhanja, S.K. and Dash, B.B. (2007). Effects of feeding raw or reconstituted high tannin red sorghum on nutrient utilisation and certain welfare parameters of broiler chickens. British poultry science. 48(2):198-204.
Lee, K.W. Everts, H. Kappert, H.J. Frehner, M. Losa, R. and Beynen, A.C. (2003). Effects of dietary essential oil components on growth performance, digestive enzymes and lipid metabolism in female broiler chickens. British poultry science. 44(3):450-457.
Lee, M.S. Kim, C.T. and Kim, Y. (2009). Green tea (–)-epigallocatechin-3-gallate reduces body weight with regulation of multiple genes expression in adipose tissue of diet-induced obese mice. Annals of Nutrition and Metabolism. 54(2):151-157.
Livak, K.J. and Schmittgen, T.D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods. 25(4):402-408.
Longstaff, M.A. and McNab, J.M. (1991). The effect of concentration of tannin-rich bean hulls (Vicia faba L.) on activities of lipase (EC 3.1. 1.3) and α-amylase (EC 3.2. 1.1) in digesta and pancreas and on the digestion of lipid and starch by young chicks. British Journal of Nutrition. 66(1):139-147.
Manach, C. Scalbert, A. Morand, C. Remesy, C. and Jimenez, L. (2004). Polyphenols: food sources and bioavailability. The American journal of clinical nutrition. 79(5):727-747.
Martel, F. Monteiro, R. and Calhau, C. (2010). Effect of polyphenols on the intestinal and placental transport of some bioactive compounds. Nutrition research reviews. 23(1):47-64.
Meydani, M. and Hasan, S.T. (2010). Dietary polyphenols and obesity. Nutrients. 2(7):737-751.
Moradalipour, A. Muhaghegh-Dolatabady, M. and Houshmand, M. (2018). Effects of Different Levels of oak acorn in the diet on Pancreatic Weight and expression of pancreatic Charboxypeptidase gene in Broiler Chickens. Accepted in Journal of Animal Science (Pajuhesh and Sazandegi), (In Farsi).
Moreno, D.A. Ilic, N. Poulev, A. Brasaemle, D.L. Fried, S.K. and Raskin, I. (2003). Inhibitory effects of grape seed extract on lipases. Nutrition. 19(10):876-879.
Nagy, H.M. Paar, M. Heier, C. Moustafa, T. Hofer, P. Haemmerle, G. Lass, A. Zechner, R. Oberer, M. and Zimmermann, R. (2014). Adipose triglyceride lipase activity is inhibited by long-chain acyl-coenzyme A. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids. 1841(4):588-594.
National Research Council (1994). Nutrient requirements of poultry (9th rev. ed). National Academy Press, Washington, DC.
Nedaei, F. Houshmand, M. Parsaei, S. and Meamar, M. (2017). Effect of oak acorn and dietary methionine level on performance, some organs weight and tibia characteristics in broiler chicken. Iranian Journal of Animal Science. 48(2):219-228. (In Farsi).
Oliveira, R.F. Goncalves, G.A. Inacio, F.D. Koehnlein, E.A. de Souza, C.G.M. Bracht, A. and Peralta, R.M. (2015). Inhibition of pancreatic lipase and triacylglycerol intestinal absorption by a pinhao coat (Araucaria angustifolia) extract rich in condensed tannin. Nutrients. 7(7):5601-5614.
Pfaffl, M.W. Horgan, G.W. and Dempfle, L. (2002). Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic acids research. 30(9):36.
Puvaca, N. Stanacev, V. Glamocic, D. Levic, J. Peric, L. and Milic, D. (2013). Beneficial effects of phytoadditives in broiler nutrition. World's Poultry Science Journal. 69(1):27-34.
Rezaei, M. and Semnaninejad, H. (2016). Effects of different levels of raw and processed oak acorn (Quercus castaneifolia) on performance, small intestine morphology, ileal digestibility of nutrients, carcass characteristics and some blood parameters in broiler chickens. Poultry Science Journal. 4(2):127-138.
Rezaei, M. Nassiri Moghaddam, H. Pour Reza, J. and Kermanshahi, H. (2004). The effects of dietary protein and lysine levels on broiler performance, carcass characteristics and N excretion. International Journal of Poultry Science. 3(2):148-152.
SAS. (2005). User’s Guide. Version 9. SAS Institute. Cary. NC.
Sinaei Kh and Houshmand M. (2016). Effects of Dietary Inclusion of Raw or Treated Iranian Oak Acorn (Quercus brantii Lindl.) on the Performance and Cecal Bacteria of Broilers. Poultry Science Journal. 4(1):73-79.
Tumova, E. and Teimouri, A. (2010). Fat deposition in the broiler chicken: a review. Scientia Agriculturae Bohemica. 41(2):122-128.
Uchiyama, S. Taniguchi, Y. Saka, A. Yoshida, A. and Yajima, H. (2011). Prevention of diet-induced obesity by dietary black tea polyphenols extract in vitro and in vivo. Nutrition. 27(3):287-292.
Wicker, C. and Puigserver, A. (1990). Expression of rat pancreatic lipase gene is modulated by a lipid-rich diet at a transcriptional level. Biochemical and biophysical research communications. 166(1):358-364.
Williams, R.J. Spencer, J.P. and Rice-Evans, C. (2004). Flavonoids: antioxidants or signalling molecules? Free radical biology and medicine. 36(7):838-849.
Yalcin, S. Ozkul, H. Ozkan, S. Gous, R. Yaşa, I. and Babacanoglu, E. (2010). Effect of dietary protein regime on meat quality traits and carcase nutrient content of broilers from two commercial genotypes. British poultry science. 51(5):621-628.
Yang, F. Lei, X. Rodriguez-Palacios, A. Tang, C. and Yue, H. (2013). Selection of reference genes for quantitative real-time PCR analysis in chicken embryo fibroblasts infected with avian leukosis virus subgroup. BMC research notes. 6(1):1.
You, Q. Chen, F. Wang, X. Luo, P.G. and Jiang, Y. (2011). Inhibitory effects of muscadine anthocyanins on α-glucosidase and pancreatic lipase activities. Journal of agricultural and food chemistry. 59(17):9506-9511.
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