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Determination of Fatty Acids in Fish, Egg and Milk: A Comparison of Different Extraction Methods

Document Type : Research Paper

Authors

1 Researcher/Animal Science Research Institute of Iran

2 Animal Science Research Institute of Iran, Karaj, Iran

3 Animal Science Research Institute of Iran

Abstract

In this study, direct methylation was compared with two conventional extraction methods (Soxhlet and Folch) in terms of extraction yield, the stability of configuration of fatty acids chemical structures and their effect on saturated and unsaturated fatty acids profile. Also, by evaluating and comparing fat extraction methods for fish, egg yolk and milk samples, the efficiency of these methods was determined according to the sample tissue. After extraction and esterification procedures, fatty acids were determined by gas chromatography method. The statistical method of ANOVA using GLM technique was applied to compare the mean of data. The fatty acid profile was subjected to analysis similarities based on cluster analysis to check how the methods would affect the fatty acid profile and to evaluate how much differences are between the methods. The obtained results indicated that the Soxhlet had the lowest efficiency for the extraction of fatty acids, especially in a sample of dry milk, but it was the highest precision compared with the Folch and direct methylation methods. It was also found that the methods have different ability to extract fatty acids based on their unsaturation degree. In general, extraction methods, in addition to affecting the fatty acid profile, have a different effect rate, depending on the sample tissue. Therefore, in order to select an effective method for extracting fat, it is necessary to consider the sample tissue.

Keywords

References
Bligh, E. G., & Dyer, W. J. (1959). A rapid method of total lipid extraction and purification. Canadian journal of biochemistry and physiology, 37(8), 911-917.
Blumenthal, M. M., & Stier, R. F. (1991). Optimization of deep-fat frying operations. Trends in Food Science & Technology, 2, 144-148.
Brondz, I. (2002). Development of fatty acid analysis by high-performance liquid chromatography, gas chromatography, and related techniques. Analytica Chimica Acta, 465(1-2), 1-37.
Caprioli, G., Giusti, F., Ballini, R., Sagratini, G., Vila-Donat, P., Vittori, S., & Fiorini, D. (2016). Lipid nutritional value of legumes: Evaluation of different extraction methods and determination of fatty acid composition. Food chemistry, 192, 965-971.
Cascant, M. M., Breil, C., Garrigues, S., de la Guardia, M., Fabiano-Tixier, A. S., & Chemat, F. (2017). A green analytical chemistry approach for lipid extraction: computation methods in the selection of green solvents as alternative to hexane. Analytical and Bioanalytical Chemistry, 409(14), 3527-3539.
Christie, W.W., Gas chromatography and lipids, Scotland, The Oily Press Ltd.
de Morais, D., dos Santos, L., Perini, J. d. L., de Aguiar, A., de Souza, N., Matsushita, M., & Visentainer, J. (2008). Comparison of diferents lipids extraction methods and fatty acids composition in goat milk. In  Central theme, technology for all: sharing the knowledge for development. Proceedings of the International Conference of Agricultural Engineering, XXXVII Brazilian Congress of Agricultural Engineering, International Livestock Environment Symposium-ILES VIII, Iguassu Falls City, Brazil, 31st August to 4th September, 2008): International Commission of Agricultural Engineering (CIGR), Institut fur Landtechnik.
Delfan-Hosseini, S., Nayebzadeh, K., Mirmoghtadaie, L., Kavosi, M., & Hosseini, S. M. (2017). Effect of extraction process on composition, oxidative stability and rheological properties of purslane seed oil. Food chemistry, 222, 61-66.
Fiori, M., Scintu, M. F., & Addis, M. (2013). Characterization of the lipid fraction in lamb meat: comparison of different lipid extraction methods. Food Analytical Methods, 6(6), 1648-1656.
Folch, J., Lees, M., & Sloane Stanley, G. (1957). A simple method for the isolation and purification of total lipids from animal tissues. J biol Chem, 226(1), 497-509.
Frankel, E. N. (2014). Lipid oxidation: Elsevier.
Kang, J. X., & Wang, J. (2005). A simplified method for analysis of polyunsaturated fatty acids. BMC biochemistry, 6(1), 5.
Kris-Etherton, P. M., Harris, W. S., & Appel, L. J. (2002). Fish consumption, fish oil, omega-3 fatty acids, and cardiovascular disease. circulation, 106(21), 2747-2757.
Lewis, T., Nichols, P. D., & McMeekin, T. A. (2000). Evaluation of extraction methods for recovery of fatty acids from lipid-producing microheterotrophs. Journal of Microbiological Methods, 43(2), 107-116.
Meier, S., Mjøs, S. A., Joensen, H., & Grahl-Nielsen, O. (2006). Validation of a one-step extraction/methylation method for determination of fatty acids and cholesterol in marine tissues. Journal of Chromatography A, 1104(1-2), 291-298.
Otero, P., Saha, S. K., Mc Gushin, J., Moane, S., Barron, J., & Murray, P. (2017). Identification of optimum fatty acid extraction methods for two different microalgae Phaeodactylum tricornutum and Haematococcus pluvialis for food and biodiesel applications. Analytical and Bioanalytical Chemistry, 409(19), 4659-4667.
Panavaitė, D., Adomavičiūtė, E., & Vičkačkaitė, V. (2006). Anovel SPME fibre for fatty acid determination. Chemija, 17(4), 61-66.
Roose, P., & Smedes, F. (1996). Evaluation of the results of the QUASIMEME lipid intercomparison: the Bligh & Dyer total lipid extraction method. Marine pollution bulletin, 32(8-9), 674-680.
Rosenfeld, J. (2002). Application of analytical derivatizations to the quantitative and qualitative determination of fatty acids. Analytica Chimica Acta, 465(1-2), 93-100.
Sahasrabudhe, M., & Smallbone, B. (1983). Comparative evaluation of solvent extraction methods for the determination of neutral and polar lipids in beef. Journal of the American Oil Chemists' Society, 60(4), 801-805.
Stibilj, V., Rajsp, M. K., & Holcman, A., (1999), Fatty acid composition of eggs enriched with omega-3 fatty acids on the market, Zootehnika, 74(2), 27-36.
Xiao, L. (2010). Evaluation of extraction methods for recovery of fatty acids from marine products. Masters Dissertion, University of Bergen.
Research Journal of Livestock Science
Volume 32, Issue 125
March 2020
Pages 71-88
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