Effect of Toxin Binder and Probiotic Addition in Diets contaminated to Aflatoxin on Performance, Aflatoxin Concentrations of Liver and Muscle and Intestinal Microbial Population of Broiler Chickens

Document Type : Research Paper

Authors

1 Department of Animal science ,Arak Branch,Islamic Azad University,Arak , Iran

2 Department of Animal Science, Arak Branch, Islamic Azad University, Arak, Iran

3 Animal Science Research Instittute of Iran, Alborz, Iran

4 Department of Animal Science, College of Agriculture and Natural Resource, Arak Branch, Islamic Azad University, Arak

Abstract

The aim of current research was to investigate the efficiency of dietary addition of ASRI1® and ASRI2® toxin binders and probiotic on performance, Aflatoxin concentrations of liver and muscle and intestinal microbial population of broiler chicks fed Aflatoxin-contaminated diets. A total of 420 one-day old Ross 308 broiler chicks were assigned in a completely randomized design to 7 treatments with 5 replications of 12 birds in each. The experimental treatments 1) non Aflatoxin contaminated diets or negative control (NC), 2) Aflatoxin-contaminated diets or positive control (PC), 3) PC diets containing ASRI1® toxin binder, 4) PC diet containing ASRI2® toxin binder, 5) PC diet containing probiotic, 6) PC diet containing probiotic+ ASRI1® toxin binder and 7) PC diet containing probiotic+ ASRI2® toxin binder. Performance was evaluated in periodical, Aflatoxin concentrations of liver and muscle at 28 and 42 days of age and intestinal microbial population at 42 days. The results showed body weight was significantly lower (p < 0.05) and feed conversion ratio was significantly higher (p < 0.05) in NC group when compared with PC group in grower and finisher periods. The harmful microbial population and Aflatoxin concentrations of liver and muscle were significantly higher (p < 0.01) in PC group in comparison to NC group. However, the dietary addition of commercial toxin binders and probiotic especially their combination, significantly alleviated (p < 0.05) Aflatoxin effects on performance, Aflatoxin concentrations of liver and muscle and intestinal microbial population.

Keywords

References

Abdallah, M.F., Girgin, G. and Baydar, T. (2015). Occurrence, prevention and limitation of mycotoxins in feeds. Animal Nutrition and Feed Technology. 15: 471-490.
Agboola, A.F., Omidiwura, B.R.O., Odu, O., Odupitan, F.T. and Iyayi, E.A. (2015). Effect of probiotic and toxin binder on performance, intestinal microbiota and gut morphology in broiler chickens. Journal of Animal Science Advances. 5: 1369-1379.
Alzawqari, M., Kermanshahi, H., Nassiri Moghaddam, H., Tawassoli, M.H. and Gilani, A. (2013). Alteration of gut microflora through citric acid treated drinking water in preslaughter male broilers. African Journal of Microbiology Research. 7: 564-567.
AOAC. (2004). Official Methods of Analysis. 18th ed., Association of Official Analytical Chemists, Washington, DC.
Attia, Y.A. (2003). Responses of growth performance, carcass characteristics, meat quality and plasma constituents of male Campbell ducks to dietary levels of methionine and phytase, and their interaction. Egyptian Poultry Science Journal. 23: 557-580.
Awad, W.A., Böhm, J., Razzazi-Fazeli, E., Faukal, K. and Zentek, J. (2006a). Effect of addition of a probiotic microorganism to broiler diets contaminated with deoxynivalenol on performance and histological alterations of intestinal villi of broiler chickens. Poultry Science. 85: 974-979.
Awad, W.A., Böhm, J., Razzazi-Fazeli, E. and Zentek, J. (2006b). Effects of feeding deoxynivalenol contaminated wheat on growth performance, organ weights and histological parameters of the intestine of broiler chickens. Journal of Animal Nutrition and Animal Physiology. 90: 32-37.
Azizpour, A.,  and  Moghadam, N. (2015). Effects of Yeast Glucomannan and Sodium Bentonite on the Toxicity of Aflatoxin in Broilers. Brazilian Journal of Poultry Science. 17:7-13.Bai, K., Huang, Q., Zhang, J., He, J., Zhang, L. and Wang, T. (2016). Supplemental effects of probiotic Bacillus subtilis fmbJ on growth performance, antioxidant capacity, and meat quality of broiler chickens. Poultry Science. 0: 1-9.
Bayankaram, P.P. and Sellamuthu, P.S. (2016). Antifungal and antiaflatoxigenic effect of probiotics against Aspergillus flavus and Aspergillus parasiticus. Toxin Review. 35: 10-15.
Beski, S.S.M. and AL-Sardary, S.Y.T. (2015). Effects of dietary supplementation of probiotic and symbiotic on broiler chickens hematology and intestinal integrity. International Journal of Poultry Science, 14: 31-36.
Bhatti, S.A., Khan, M.Z., Saleemi, M.K. and Saqib, M. (2016). Aflatoxicosis and ochratoxicosis in broiler chicks and their amelioration with locally available bentonite clay. Pakistan Veterinary Journal. 36: 68-72.
Bilal, T., Aksakal, D.H., Sunnetci, S., Keser, O. and Eseceli, H. (2014). Detection of aflatoxin, Zearalenone and deoxynivalenol in some feed and feedstuffs in Turkey. Pakistan Veterinary Journal. 34: 459-463.
Celyk, K., Denly, M. and Savas, T. (2003). Reduction of toxic effects of Aflatoxin B1 by using baker yeast (Sacharomyces cerevisiae) in growing broiler chicken diets. Revista Brasileira Zootecnia. 32: 615-619.
Gowda, N.K.S., Ledoux, D.R., Rottinghaus, G.E., Bermudez, A.J. and Chen, Y.C. (2008). Efficacy of turmeric (Curcuma longa), containing a known level of curcumin, and a hydrated sodium calcium aluminosilicate to ameliorate the adverse effects of Aflatoxin in broiler chicks. Poultry Science. 87: 1125-1130.
Griggs, J.P. and Jacob, J.P. (2015). Alternatives to antibiotics for organic poultry production. Journal of Applied Poultry Research. 14: 750-756.
Han, W., Zhang, X.L., Wang, D.W., Li, L.Y., Liu, G.L and Zhao, Y.X. (2013). Effects of microencapsulated Enterococcus faecalis CG1.0007 on growth performance, antioxidation activity, and intestinal microbiota in broiler chickens. Journal of Animal Science. 91: 4374-4382.
Heo, J.M., Opapeju, F.O., Pluske, J.R., Kim, J.C., Hampson, D.J. and Nyachoti, C.M. (2012). Gastrointestinal health and function in weaned pigs: a review of feeding strategies to control post-weaning diarrhea without using in-feed antimicrobial compounds. Journal of Animal Physiology and Animal Nutrition. 97: 207-237.
Hussain, Z., Zargham Khan, M., Khan, A., Javed, I., Saleemi, M., Mahmood, S. et al. (2010).  Residues of Aflatoxin B1 in broiler meat: Effect of age and dietary Aflatoxin B1 levels. Food and Chemical Toxicology. 48: 3304-3307.
Idahore, K.O. (2013). Alternative feedstuffs utilisation in Nigerian poultry industry: potentials, problems and prospects. Worlds Poultry Science Journal. 69: 666-675.
Khan, A., Aalim, M.M., Khan, M.Z., Saleemi, M.K., He, C., Khatoon, A., et al. (2017). Amelioration of immunosuppressive effects of Aflatoxin and Ochratoxin A in white Leghorn layers with distillery yeast sludge. Toxin Review. 1: 1-7.
Lee, K., Lillehoj, H.S. and Siragusa, G.R. (2010). Direct-Fed microbials and their impact on the intestinal microflora and immune system of chickens. Japanese Poultry Science. 47: 106-114.
Marin, D.E. and Taranu, I. (2015). Ochratoxin A and its effects on immunity. Toxin Review. 34: 11-20.
Mogadam, N. and Azizpour, A. (2011). Ameliorative effect of glucomannan-containing yeast product (Mycosorb®) and sodium bentonite on performance and antibody titers against Newcastle disease in broilers during chronic aflatoxicosis. African Journal of Biotechnology. 10: 17372-17378.Mookiah, S., Sieo, C.C., Ramasamy, K., Abdullah, N. and Ho, Y.W. (2014). Effects of dietary prebiotics, probiotic and synbiotics on performance, caecal bacterial populations and caecal fermentation concentrations of broiler chickens. Journal of Science, Food and Agriculture. 94: 341-348.
Nemati, Z., Karimi, A. and Besharati, M. (2015). Effects of Aflatoxin B1 and yeast cell wall supplementation on the growth performance of broilers. International Conference on Innovations in Chemical and Agricultural Engineering (ICICAE’2015); 2015 Feb 8-9; Kuala.
Naseem, M.N., Saleemi, M.K., Khan, A., Khatoon, A., Gul, S.T., Rizvi, F., Ahmad, I., and Fayyaz, A. (2018). Pathological effects of concurrent administration of aflatoxin B1 and fowl adenovirus-4 in broiler chicks. Microbial Pathogenesis. 121:147-154.
Panda, A.K., Rama Rao, S.V., Raju, M.V. and Shyam Sunder, G. (2009). Effect of butyric acid on performance, gastrointestinal tract health and carcass characteristics in broiler chickens. Asian-Australasian Journal of Animal Sciences. 22: 1026-1031.
Rezar, V., Frankič, T., Narat, M., Levart, A. and Salobir, J. (2007). Dose-dependent effects of T-2 toxin on performance, lipid peroxidation, and genotoxicity in broiler chickens. Poultry Science. 86:1155-1160.
Ross Broiler Management Manual. (2007). Ross 308 Broiler: Nutrition Specification. Aviagen, UK.
Saleemi, M.K., Khan, M.Z. and Khan, A. (2017). Study of fungi and their toxigenic potential isolated from wheat and wheat bran. Toxin Review. 36: 80-88.
Salem, R., El-Habashi, N., Fadl, S.E., Sakr, O.A. and Elbialy, Z.I. (2018). Effect of probiotic supplement on aflatoxicosis and gene expression in the liver of broiler chicken. Environmental Toxicology and Pharmacology. 60: 118-127.
SAS. (2001). SAS User’s Guide: Statistics. Version 9.2, SAS Institute Inc., NC., USA.
Shotwell, O.L., Hesseltine, C.W., Stubblefield, R.D. and Sorenson, G.W. (1966). Production of Aflatoxin on rice. Applied Microbiology. 14: 425-428.
Wang, R.J., Fui, S.X., Miao, C.H. and Feng, D.Y. (2006). Effects of different mycotoxin adsorbents on performance, meat characteristics and blood profiles of avian broilers fed mold contaminated corn. Asian-Australian Journal of Animal Science. 19: 72-79.
Zarghi, H., Golian, A., Kermanshahi, H., Raji, A.R. and Heravi, A. (2012). The effect of dietary levels of triticale and enzyme supplementation on performance, gut morphology and blood chemistry of very young broiler chicks. Iranian Journal of Animal Science Research. 3: 324-334.
Zhang, C., Li, F.Y., Liu, W.H., Zou, L., Yan, C. and Lu, K. (2013). T4-Like Phage Bp7, a potential antimicrobial agent for controlling drug-resistant Escherichia coli in chickens. Applied Environmental Microbiology. 79: 5559-5565.
Zhang, L., Zhang, L., Zhan, X., Zeng, X., Zhou, L., Cao, G. et al. (2016). Effects of dietary supplementation of probiotic, Clostridium butyricum, on growth performance, immune response, intestinal barrier function, and digestive enzyme activity in broiler chickens challenged with Escherichia coli K88. Journal of Animal Science and Biotechnology. 7: 10-25.
Animal Sciences Journal
Volume 33, Issue 126
June 2020
Pages 117-128

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