Document Type : Research Paper

Authors

1 department of animal science, shahr-e-qods branch, islamic azad university

2 واحد علوم و تحقیقات

Abstract

In this research, effect of treating native rapeseed meal (NRSM) with microwave irradiation on chemical composition, glucosinolate, phytic acid, ruminal dry matter (DM) and crude protein (CP) degradability, metabolizable protein (MP) and in vitro CP digestibility of before and after irradiation were investigated. At first, moisture content of samples (three sample of 500 g) were increased to 250 g/kg. Then, were subjected to microwave irradiation at a power of 800 W for 2, 4 or 6 min. Chemical composition, phytic acid and glucosinolate contents of untreated and irradiated samples were determined by standard methods. Degradation kinetics of CP were determined according to in situ procedure. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was used to monitor protein subfractions and the fate of true proteins of untreated and irradiated feed samples in the rumen. The results of experience showed that irradiation decreased the total glucosinolate and phytic acid content of NRSM (p < 0.001). Ruminal disappearance of DM and CP in different incubation times linearly increased (p < 0.05) as irradiation time increased. Based on results of this study, microwave irradiation more than 2 min decreased glucosinolate and phytic acid content and ruminal degradability of CP and increased by pass protein, in vitro CP digestibility and MP of NRSM and eventually, microwave irradiation was effective in improving nutritional value of NRSM for using in ruminant diet.

Keywords

ابراهیمی محمودآباد، س.ر.، نیکخواه، ع. و صادقی، ع.ا. (1395). ترکیبات ضدتغذیه‌ای و تجزیه‌پذیری شکمبه‌ای ماده خشک و پروتئین خام دانه منداب بومی عمل‌آوری شده با پرتو گاما و میکروویو. نشریه پژوهش‌های علوم دامی ایران. 8 (1): ص ص 85-72.
باشتنی، م.، فرهنگ فر، ه. و گنجی، ف. (1397). تاثیر فرایند پلت بر ترکیب شیمیایی، بخش های نیتروژن دار و خصوصیات تجزیه پذیری کنسانتره تجاری با استفاده از دو روش برون تنی. نشریه پژوهش‌های علوم دامی. 28(2): ص ص 35-50.
 پایا، ح.، تقی زاده، ا.، جانمحمدی، م.، مقدم، غ.ع. و حسینخانی، ع. (1395). اثر پرتوتابی با میکروویو بر ناپدید شدن شکمبه‌ای و روده‌ای ماده خشک و پروتئین خام دانه گلرنگ با استفاده از روش کیسه‌های نایلونی و کیسه‌های نایلونی متحرک. نشریه پژوهش‌های علوم دامی. 26 (2): ص ص 130-121.
پیر عدل، ا.، میر محمدی، ر. و خلیل وندی بهروزیار، ح. (1396). بررسی تغییرات شیمیایی، روند تجزیه‌‌پذیری شکمبه‌ای و هضم روده‌ای نشاسته و پروتئین خام واریته‌های مختلف دانه جو پرتوتابی شده با میکروویو. نشریه پژوهش در نشخوارکنندگان. 5 (4): ص ص 144-119.
جلیلیان، س.، فتاح نیا، ف.، شورنگ، پ. و محمد زاده، ح.( 1394). اثر روش‌های مختلف پرتوتابی بر بخش‌های مختلف پروتئین و تجزیه‌پذیری شکمبه‌ای و قابلیت هضم روده‌ای پروتئین کنجاله آفتابگردان. نشریه پژوهش‌های علوم دامی. 25 (2): ص ص 80-69.
شورنگ، پ.، نیکخواه، ع.، زارع شحنه، ا. و رئیسعلی، غ. ر. (1385). مطالعه اثرات پرتوتابی بر ناپدید شدن شکمبه ای و پس شکمبه ای پروتئین برخی مواد خوراکی با استفاده از تکنیک کیسه های نایلونی و الکتروفورز ژل پلی آکریلامید. رساله دکتری، دانشکده کشاورزی، دانشگاه تهران.
طحان، ق.، فتحی نصری، م.ح.، ریاسی، ا.، بهگر، م. و فرهنگ فر، ه. (1390). اثر پرتوتابی الکترونی بر فراسجه های تجزیه پذیری و قابلیت هضم شکمبه ای و پس شکمبه ای ماده خشک و پروتئین خام برخی مواد خوراکی. نشریه پژوهش‌های علوم دامی ایران. 3(4): ص ص 434-422.
قنبری، ف.، قورجی، ت.، شورنگ، پ.، منصوری، ه. و تربتی نژاد، ن. (1393). تاثیر پرتوتابی بر ناپدید شدن شکمبه‌ای ماده خشک و پروتئین خام و قابلیت هضم برون تنی کنجاله کانولا. نشریه علوم دامی (پژوهش و سازندگی). 26:ص ص 66-55.
هدایتی، ع.خ.، امانلو، ح.، نیکخواه، ع.، امیرآبادی فراهانی، ط. و اسلامیان فارسونی، ن. (1391). مقایسه ارزش غذایی کنجاله منداب و کنجاله تخم پنبه در تغذیه گاوهای هلشتاین. مجله علوم دامی ایران. 43 (1). 70-61.
AFRC. (1992). Nutritive requirements of ruminant animal: Protein. Nutrition Abstracts and Reviews. 62: 787-835. CAB. International Wallingford.
Alajaji, S.A. and El-Adawy, T.A. (2006). Nutritional composition of chickpea (Cicer arietinum L.) as affected by microwave cooking and other traditional cooking methods. Journal of Food Composition and Analysis. 19: 806–812.
AOAC. (1995). Official Methods of Analysis, 16th Ed. Association of Official Analytical Chemists. Arlington, VA, USA.
Banik, S., Bandyopadhyay, S. and Ganguly. S. 2003. Bio effects of microwave-a brief review. Bioresource Technology. 87: 155-159.
Bhatty, R.S., McKenzie, S.L. and Finlayson, A.J. (1999). The proteins of rapeseed soluble in salt solutions. Canadian Journal of Biochemistry. 46, 1191-1197.
Calsamiglia, S. and Stern, M.D. (1995). A three-step in vitro procedure for estimating intestinal digestion of protein in ruminants. Journal of Animal Science. 73: 1459-1465.
Clifford, A. and Smith, D.V. (1987). Rapid method for determining total glucosinolates in rapeseed by enzimatically released glucose. Journal of the Science of Food and Agriculture. 38: 141-150.
De Boland, A.R., Garner, G.B. and O Dell, B.L. (1975). Identification and properties of phytate in cereal grains and oilseed products. Journal of Agriculture and Food Chemistry. 23: 1186- 1189.
Duskova, D., Dvorak, R., Rada, V., Doubek, J. and Marounek, M. (2001). Concentration of phytic acid in faeces of calves fed sterter diets. Acta Veterinaria Brno. 70: 381-385.
Ebrahimi, S.R., Nikkhah, A. and Sadeghi, A.A. (2010). Changes in nutritive value and digestion kinetics of canola seed due to microwave irradiation. Asian-Australasian Journal of Animal Science. 27: 347-354.
Folawiyo, Y.L. and Apenten, R.K.O. (1997). The effect of heat and acid treatment on the structure of rapeseed albumin (napin). Food Chemistry. 58: 237-243.
Gozho, G.H., McKinnon, J.J., Christensen, D.A., Racz, V. and Mustsvangwa, T. (2009). Effect of type of canola protein supplement on ruminal fermentation and nutrient flow to the duodenum in beef heigers. Journal of Animal Science. 87: 3363-3371.
Kala, B.K. and Mohan, V.R. (2012). Effect of microwave irradiation on the antinutritional of two accessions of velvet bean, Mucuna pruriens (L.,) DC. Var. utilis (Wall. ex Burck). International Food Research. 19 (3): 961-969.
Huang, S., Liang, M., Lardy, G., Huff, H.E., Kerley M.S. and Hsieh, F. (1995). Extrusion process of rapeseed meal for reducing glucosinolates. Animal and Feed Science and. Technology. 56: 1-9.
Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of head of bacteriophage T4. Nature, 227, 680-685.
Lardy, G.P. and Kerley, M.S. (1994). Effect of increasing the dietary level of rapeseed meal on intake by growing beef steers. Journal of Animal Science. 72: (8) 1936-1942.
Mandiki, S.N.M., Derycke, G.J., Bister, L., Mabon, N., Wathelet, J.P., Marlier, M. and Paquay, R. (2002). Chemical changes and influence of rapeseed antinutritional factor on gestating and lactating ewes. Part 1. Animal performances and plasma hormones and glucose. Animal and Feed Science and. Technology. 98: 25–35.
Michalet-Doreau, B. and Ould-Bah, M.Y. (1992). In vitro and in sacco methods for the estimation of dietary nitrogen degradability in the rumen: a review. Animal and Feed Science and. Technology. 40: 57-86.
Murray, R.K., Granner, D.K., Mayes, P.A. and Rodwell, V.W. (2003). Harper’s Biochemistry, 26th Ed. McGraw-Hill, New York, NY, USA.
National Research Council. (2001). Nutrient Requirements of Dairy Cattle. 7th Ed. National Academy of Sciences, Washington, DC. Ananymous.
Oerlemans, K. Barrett, D.M. Suades, C.B. Verkerk, R. and Dekker. M. (2006). Thermal degradation of glucosinolates in red cabbage. Food Chemistry. 95: 19–29.
Oliveira, M.E.C. and Franca, A.S. (2002). Microwave heating of foodstuffs. Journal of Food Engineering. 53: 347-359.
Ørskov E.R. and McDonald, I. (1979). The estimation of protein degradability in the rumen from incubation weighed according to rate of passage. Journal of Agriculture Science (Camb.). 92: 499-503.
Peng, Q., Khan, N.A., Wanng, Z. and Moist, D. (2014). Dry heating induced changes in protein molecular stracture, protein subfractions and nutrient profiles in camelina seed. Journal of Dairy Science. 97: 446-457.
PHOTO-CAPT. 1999. V. 99. B.P. 66 TORCY. http://www.vilber.com
Sadeghi, A.A. and P. Shawrang, (2006). Effects of microwave irradiation on ruminal degradability and in vitro digestibility of canola meal. Animal Feed Science and Technology. 127: 45-54.
Sadeghi, A.A. and Shawrang, P. (2007). Effects of microwave irradiation on ruminal protein degradation and intestinal digestibility of cottonseed meal. Livestock Science. 106: 176–181.
Samadi, S. and Yu. P. (2011). Dry and moist heating-induced changes in protein molecular  structure, protein subfraction, and nutrient profiles in soybeans. Jouranal of dairy Science. 94: 6092-6102.
SAS. (1996). Statistical Analysis System. SAS Intit. Inc., Cary, NC, USA.
Siddhuraju, P., Makkar, H.P.S. and Becker, K. (2002). The effect of ionising radiation on antinutritional factors and the nutritional value of plant materials with reference to human and animal food. Food Chemistry. 78: 187-205.
Taghinejad-Roudbaneh, M., Kazemi-Bonchenary, M., Salem, A.Z.M. and Kholif, A.E. (2016). Influence of rosting, gamma ray irradiation and microwaving on ruminal dry matter and crude protein digestion of cottonseed. Italian Journal of Animal Science. 15 (1): 144-150.
Van Soest, P.J. (1994). Nutritional Ecology of the Ruminants. 2nd Edition. Cornell University Press. NY. USA.
Van Soest, P.J., Robertson, J.B. and Lewis, B.A. (1991). Methods for dietary fiber, neutral detergent fiber and nonstarch polysaccharidesin relation to animal nutrition. Journal of Dairy Science. 74: 3583–3597.
Voragen, A.G.J., Gruppen, H., Marsman, G.J.P. and Mul, A.J. (1995). Effect of some manufacturing technologies on chemical, physical and nutritional properties of feed. In: Recent Advances in Animal Nutrition (Ed. P.C. Gransworthy and D.J.A. Cole, (Eds), Nottingham university press, Nottingham, UK, pp. 93-126.
Yan, X., Khan, N.A., Zhang, F., Yang, L. and Yu. P. 2014. Microwave irradiation induced changes in protein molecular structures of barley grains: relationship to changes in protein chemical profile, protein subfractions, and digestion in dairy cows. Journal of Agriculture and Food Chemistry. 62 (28): 6546-6555.