The effect of Aloe vera gel on performance, intestinal microbial population, blood metabolites, antibody titers against sheep red blood cells and white blood cell counts in broiler chickens.

Kooshesh, Mina; Salarmoini, Mohamad; Afsharmanesh, Mohsen; Tavakkoli, Hadi

doi:10.22092/asj.2019.124655.1843

Document Type : Research Paper

Authors

¹ Department of Animal Science, College of Agriculture, Shahid Bahonar University of Kerman. Kerman, Iran

² ِDepartment of Animal Science, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran

³ Department of Animal Science, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran.

⁴ Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.

https://doi.org/10.22092/asj.2019.124655.1843

Abstract

This experiment was conducted in a completely randomized design with 5 treatments, 4 replicates and 10 Ross 308 broiler chicks per replicate. The treatments consisted of: 1) control (basal diet) 2) basal diet + supplemented with 600 mg/kg antibiotic flavophospholipol 3, 4 and 5) chicks fed basal diet but 5, 10, and 15 gram/ liter Aloe vera gel added to their drinking water, respectively. In the whole of the experimental period, the treatments did not have any significant effect on body weight gain, feed intake, feed conversion ratio, and European efficiency factor in broilers (P>0.05). Intestinal microbial population (Escherichia coli, Lactobacillus) was not affected by the experimental treatments (P>0.05). Blood metabolites included cholesterol, triglyceride, high-density lipoprotein, low-density lipoprotein, albumen and alkaline phosphatase were not affected by the experimental treatments (P>0.05), except serum glucose level which significantly reduced in birds received 15 gr /liter Aloe vera jel compared to the control (p < 0.05). Effect of the experimental treatments on the antibody titers against sheep red blood cells at 28 and 42 days of age were not significant (P>0.05). Also, in the case of white blood cells, the heterophile and heterophile to lymphocyte ratio were increased in the bird received different levels of Aloe vera (p < 0.01). In general, the use of the Aloe vera gel could improve immune response.

Keywords

20.1001.1.25886436.1399.33.127.1.3

References

جعفرزاده، ا.، درمانی، ح.، حسین زاده، ن.، روستایی، م. (1392). اثر سطوح مختلف پودر ژل آلوئهورا بر عملکرد، میکرو فلور روده و اندامهای گوارشی جوجه بلدرچین ژاپنی. نشریه علوم دامی. شماره. 106. ص 242 -231.

مختاری، ا.، اکبری، م.ر.، اسدی خشویی، ا. (1395). اثر افزودن پودر سیر و سیر تازه به جیره بر عملکرد و پاسخ ایمنی جوجه‌های گوشتی. پژوهشهای تولیدات دامی. شماره 13. ص 31-24.

مهدوی، ع.، صابری، م.، جلودار، غ.، شهروزیان، ا. (1395) . اثرات سطوح مختلف ژل آلوئهورا روی فراسنجههای هماتولوژی، بیوشیمیایی، ایمونولوژی در مدل جوجه. نشریه کومش. صفحه 135 - 143 .

Akhtar, M., Hai, A., Awais, M.M., Iqbal, Z., Muhammad, F. and Ul Haq, A. (2012). Immunostimulatory and protective effects of Aloe vera against coccidiosis in industrial broiler chickens. Veterinary Parasitological, 11: 59-67.

Aviagen (2014). Nutrition specification for Ross 308. Aviagen Limited, Newbridge, Scotland.

Besharatian, M., Arshami, J., Valizade, R., Tahmasebi, A. and BahariKashani, R. (2012). Effects of Aloe vera leaf powder and extract on immune response in broilers. Proc. 5th Iranian Congress on Animal Science, Isfahan, Iran, pp, 366–370.

Choi, S. and Chung, M.H. (2003). A review on the relationship between Aloe vera components and their biologic effects. Seminars in Integrative Medicine, 1: 53–62.

Christaki, E.V. and Florou – Paneri, P.C. (2010). Aloe vera: A plant for many uses. Journal Food Agriculture, Environ, 8: 245–249.

Darabighane, B., Zarei, A., Zare Shahneh, A. and Mahdavi, A. (2011). Effects of different levels of Aloe vera gel as an alternative to antibiotic on performance and ileum morphology in broilers. Italian Journal of Animal Science, 10: 189-194.

Djeraba, A. and Quere, P. (2000). In vivo macrophage activation in chickens with acemannan, a complex carbohydrate extracted from Aloe vera. International Journal Immunopharmaco, 22: 365–372.

Durrani, F.R., Sanaullah, N., Chand, Z. and Akhtar, S. (2008). Using aqueous extract of aloe gel as anticoccidial and immunostimulant agent in broiler production. Sarhad Journal Agriculture, 24(4): 665-669.

Fallah, R. (2015). Effect of adding Aloe Vera Gel and Garlic powder on performance and liver functions of broiler chickens. Global Journal of Animal Scientific Research. 3: 491-496.

Hamman, J.H. (2008). Composition and applications of Aloe vera leaf gel. Molecules, 13: 1599–1616.

Harlev, E., Nevo, E., Lansky, E.P., Ofir, R. and Bishayee, A. (2012). Anticancer potential of Aloes: antioxidant, antiproliferative, and immunostimulatory attributes. Planta Med, 78: 843–852.

Jagmohan Singh, K.M. and Koley, S. (2014) .Effects of Aloe Vera on growth performance Parameters of Broiler Chickens. Indian Veterinary Journal, 12: 78 -79.

Jiang, L., Feng, Y., Yang, X., Zhou, X. and Yang, D. (2005). Effects of gel, polysaccharide and acemannan from Aloe vera on broiler gut flora, microvilli density, immune function and growth performance. Chinese Journal Veterinary Science, 25: 668–671.

Karaca, K., Sharma, J.M. and Nordgren, R. (1995). Nitric oxide production by chicken macrophages activated by Acemannan, a complex carbohydrate extracted from Aloe vera. International Journal Immunopharmaco, 17:183–188.

Li, Y.L. (1991). Culture Medium Manual. Jilin Science and Technology Press, Changchun, China.

Mahdavi, A., Alemi, F., Ghazvinian, K., Ghaderi, M. and Darabighane, B. (2012). Study of effects of different levels of Aloe vera gel powder on antibody titre against sheep red blood cells and other blood parameters in broilers. British Poultry, 8: 49–50.

McAnalley, B.H. (1989). Process for preparation of aloe products, Google Patents.

Mehala, C. and Moorthy, M. (2008). Effect of Aloe vera and Curcuma longa (turmeric) on carcass characteristics and biochemical parameters of broilers. International Journal of Poultry Science, 7: 857–861.

Mereole, F.U.C. (2011).Evaluation of the dietary inclusion of Aloe vera as an alternative to antibiotic growth promoter in broiler production. Pakistan Journal of Nutrition, 10: 1-5.

Mitsch, P., Zitterl-Eglseer, K., Kohler, B., Gabler, C., Losa, R. and Zimpernik, I. (2004). The effect of two different blends of essential oil components on the proliferation of Clostridium perfringens in the intestines of broiler chickens. Poultry Science, 83: 669-675.

Naghi Shokri, A., Ghasemi, H.A. and Taherpour, K. (2016). Evaluation of Aloe vera and synbiotic as antibiotic growth promoter substitutions on erformance, gut morphology, immune responses and blood constitutes of broiler chickens. Animal science journal, 12 -17.

NRC (1994). Nutrient requirements of poultry. 9th Rev Ed, National Acad. Press, Washington, DC, USA.

Odo, B.I., Ekenyem, B.U. and Nwamo, A.C. (2010). Effects of Aloe vera as leaf protein concentrate on growth performance of cockerels. International Journal of Poultry Science, 9, 426-428.

Reynolds, T. and Dweck, A.C. (1999). Aloe vera leaf gel: A review update. Journal of Ethnopharmacology, 68: 3-37.

Salary, J., Kalantar, M., Sahebi ala, M., Ranjbar, K. and Hemati H.R. (2014). Drinking water supplementation of licorice and aloe vera extracts in broiler chickens. Scientific Journal of Animal Science, 3(2): 41-48.

SAS Institute (2003). SAS User’s Guide. Version 9. SAS Institute Inc. Cary. NC.

Valle – Paraso, M., Vidamo, P., Anunciado, R. and Lapitan, A. (2005). Effects of Aloe vera (Aloe barbadensis) on the white blood cell count and antibody titre of broiler chickens vaccinated against Newcastle disease. Philipp Journal of veterinary Medicine, 42: 49–52.

Vogler, B.K. and Ernst, E. (1999). Aloe vera: a systematic review of its clinical effectiveness. British Journal of General Practice, 49: 823-831.

Wegmann, T.C. and Smithies, O. (1966). A simple hemagglutination system requiring small amounts of red cells and antibodies. Transfusion, 6(1): 67-73.

Wittow, G.C. (2000). Sturkies Avian Physiology. 5thed. Academic press, California.

Yim, D., Kang, S.S., Kim, D.W., Kim, S.H., Lillehoj, H.S. and Min, W. (2011). Protective effects of Aloe vera-based diets in Eimeria maxima-infected broiler chickens. Experimental Parasitology, 127:322–325.

Zhang, L. and Tizard, I.R. (1996). Activation of a mouse macrophage cell line by acemannan: The major carbohydrate fraction from Aloe vera gel. Immunopharmacology, 35: 119–128.

Research Journal of Livestock Science

The effect of Aloe vera gel on performance, intestinal microbial population, blood metabolites, antibody titers against sheep red blood cells and white blood cell counts in broiler chickens.

References

References

Volume 33, Issue 127
September 2020
Pages 3-12

The effect of Aloe vera gel on performance, intestinal microbial population, blood metabolites, antibody titers against sheep red blood cells and white blood cell counts in broiler chickens.

References

References

Volume 33, Issue 127September 2020Pages 3-12

Volume 33, Issue 127
September 2020
Pages 3-12