Document Type : Research Paper
Authors
1 Associate Professor Dep of Animal science of Khuzestan Ramin Agriculture and Natural Resources University, Ahvaz, Iran
2 Department of Animal Science, Mashhad university, Mashhad, Iran
3 Institute of Biotechnology, Shiraz University Shiraz, Iran.
4 Department of animal science, Zabol university, Zabol, Iran
Abstract
In this study, the polymorphism of four microsatellite loci BMS1350, LGB, ILSTS45, and BMS1915 in chromosome 3 and their association with wool trait were investigated in Baluchi sheep. DNA extraction was performed from blood samples of 185 Baluchi sheep from Abbas Abad Breeding Station. Gene fragments of microsatellites were amplified by polymerase chain reaction with specific primers. The result shown allele E in BMS1915 locus with 0.40, Allele D in BMS1350 locus with 0.30, Allele B in LGB locus with 0.48 and Allele A in ILSTS4 locus with 0.46, had the highest frequency. The results of the Comparison of mean shown the LGB locus has the highest and ILSTS45 locus has the lowest production. Also, genotype AC in the LGB locus highest (1308 ±95.7) and genotype BC had the lowest (875 ± 54.7) of production. By comparing the coefficient of variation 4 loci founded that the greatest diversity and dispersion associated with the ILSTS45 and the lowest diversity associated with BMS1350 locus. Chi-square test (χ2) shown all of the microsatellite loci were in the Hardy-Weinberg equilibrium (P>0.05). Association analysis of polymorphism these loci shown there is no significant association between polymorphism of these loci and wool trait in Baluchi sheep (P>0.05). Statistical analysis showed there was no significant difference between loci polymorphisms and wool traits. Therefore, concluded in further research on selected studies based on molecular markers, the relationship between these loci and other traits in the Balochi sheep can be examined.
Keywords
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