Research Journal of Livestock Science

Current Issue

By Issue

By Author

By Subject

Author Index

Keyword Index

About Journal

Aims and Scope

Editorial Board

Publication Ethics

Indexing and Abstracting

Related Links

FAQ

Peer Review Process

Journal Metrics

News

The effect of β-Lactoglobulin gene polymorphism on the breeding value of milk production trait in Brown Swiss cattles

Document Type : Research Paper

Authors

1  Member of Scientific Board, Dept. of Biotechnology, Animal Science Research Institute of Iran (ASRI)

2 Department of animal science, Golpayegan Branch, Islamic Azad University, Golpayegan Branch, Islamic Azad university,Golpayegan, Iran

3 Member of Scientific Board, Dept. of Biotechnology, Animal Science Research Institute of Iran (ASRI)

Abstract

The β-lactoglobulin gene (β-LG) has been considered to be one of the most important genes involved in the milk productin related traits. In the present research, the association of β-LG polymorphism with breeding values for milk production related traits was investigated in Brown Swiss cattle. The data of this study was collected from the Golpayegan dairy farm. In this study, genomic DNA was extracted from 150 blood samples by modified salting out method. Following this stage, a fragment of 247 bp was amplified and used for enzymatic digestion.
The HaeIII restriction enzyme was used to digest of 247 bp of gene. The results showed that genotypes AA, AB and BB of the β-LG gene were estimated to have frequencies of 0.24, 0.48 and 0.28, respectively. These Genotypes were distributed according to the Hardy-Weinberg equilibrium.Genetic parameters were estimated by the restricted maximum likelihood (REML) method using DFREML program. Heritability and repeatability for milk yield traits were estimated to be 0.154 and 0.309, respectively. The statistical analysis based on the General Liner Models revealed that there is no significant effect for β-LG genotypes on the milk yield breeding values.

Keywords

References
دادار و همکاران.1382. مقایسه عملکرد تولید شیر و صفات تولید مثلی گاو هلشتاین و براون سویس در شرایط پرورش صنعتی. گزارش نهایی طرح تحقیقاتی
ضمیری،م.ج.1383. پرورش گاو شیری.انتشارات دانشگاه شیراز
Aggrey,S.E., Sabour, M.P., Lin, C.Y., Zadworney D., and Kuhnlein, U. (1998). Analysis of β-lactoglobulin locus, using the grand-daughter design in the Canadian Holstein population. Canadian Journal of Anim Science.78:245-248
Alexander, L.,.Hayes, J.G., Pearse, M.J., Bawden, W., Stewartand, A.F. and Mackinlay, A.G.(1993). Complete nucleotide sequence of the bovine β-lactoglobuline gene. Animal Biotech. 4(1), 1- 10
Alexander. L., Hayes, J.G.,  Pearse, M.J., Beattie, C.W.,  Stewart, A.F.,  Willis, I.M.  and A.G.Mackinlay.(1989). Complete sequence of the bovine β-lactoglobulin cDNA. Nucleic Acids
Barbosa, S. B.P., et al.  (2019). Genetic association of variations in the kappa-casein and β-lactoglobulin genes with milk traits in girolando cattle. Revista Brasileira de Saúde e Produção Animal, 20, e0312019.https://dx.doi.org/10.1590/s1519-9940200312019
Bovenhuis. H., Van Arendonk, J.A.M., and Korver, S.( 1992). Associations between milk protein polymorphisms and milk production traits. J. Dairy Sci. 75: 2549-2559
ČÍTEK JINDŘICH, H.L., et al. (2019). Polymorphisms in CSN3, CSN2 and LGB Genes and Their Relation to Milk Production in Dairy Cattle in the Czech Republic. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 67(1): 19 – 24
Cowan,C.M., Dentone, M.R..,and Covel, T.(1992). Chromosome substitution effects associated with k-casein and β- lactoglobulin in Holstein cattle. J. Dairy Sci. 75: 1097-1104
Dogru, U.(2015). β-Lactoglobulin genetic variants in Brown Swiss Dairy cattle and their association with milk yield and quality traits.The Journal of Animal & Plant Sciences, 25(2): 595-598
Eser, K. G. (2011). Association between milk protein polymorphism and milk production traits in Black and White dairy cattle in Turkey. Afr. J. of Biotech. 10(6):1044-1048
Gibson, K.D. and Dechow, C. D.(2018). Genetic parameters for yield, fitness, and type traits in US Brown Swiss dairy cattle.Journal of Dairy Science, Volume 101, Issue 2, 1251 - 1257
Ikonen T., Oiala, M., and Ruottinen, O.(1999). Associations between milk protein polymorphism and first lactation milk production traits in finish ayrshire cows, J. Dairy Sci. 82: 1026-1033
Medrano, J.F., and  Aguilar-Cordova, E.(1990). Polymerase chain reaction amplification of bovine β-lactoglobulin genomic sequences and identification of genetic variants by RFLPanalysis. Anim. Biotech. 1(1): 73-77
Meyer, K. (1998). DFREML version 3.0. CD-ROM Sixth World Congr. Genet. Appl. Livest. Prod.
Mercier, J.C., and Vilotte, J.L.(1993). Structure and function of milk protein genes. J. dairy sci.  76:3079-3098
Nei, M.(1978). Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89, 583–90
Ojala, M,. Erhardt, G., Ruottinen ,O., and Tlkonen,O.(1996). Allele frequencies of the major milk proteins in the finnish Ayrshire and detection of new k-casein variant. Animal Genetics, 181-77,179.
Samore A, Rizzi, R., Rossoni, A., and Bagnato, A.(2010). Genetic parameters for functional longevity,type triats, somatic cell scores, milk flow and production in the Italian brown swiss. Italian journal of animal science vol9.e.28:145-152
Stralkowska.N., Kryzyzewski.J.,Zwierzchowski.L and Ryniewicz.Z .(2002). Effects of k-Casein , β-Lactoglobulin loci polymorphism ,cow's age, stage of lactation and somatic cell count on daily milk yield and milk comostion in polish black and white cattle; animal science 20 (1), 21-35.
Tolenkhomba, T.T, Mayengbam,  P., and Yadav, B.R.( 2014). Effect of beta lactoglobulin genotypes on milk production traits in sahiwal cattle. Indian J. Anim. Res., 48 (2) : 99-102, 2014
Tsiaris, A.M., Bargouli, G.G.,  Banos, G., and Boscos, M.(2005). Effects of kappa-casein and β-lactoglobulin loci on milk production traits and reproductive performance of Holstein cows. J. Dairy Sci.88: 327-334
Zaglool A.W,  Awad. A,  sayed El Araby ,E., and Mohamed El-Bayomi, Kh.( 2016). Association of β –Lactoglobulin Gene Polymorphism with Milk Yield, Fat and Protein in Holstein-Friesian Cattle. World Vet. J. 6(3): 117-122
Zulkadir,U., Aytekin,I., and Pala,A.(2009). Genetic analyses for milk yield, lactation period and fat percentage in brown swiss cattle. Journal of animal veterinary advance. 8:857-862.
Research Journal of Livestock Science
Volume 33, Issue 129
March 2021
Pages 25-32
Files
Share
How to cite
Statistics