Effects of aromatase inhibition by Letrozole on Ross 308 broiler chicken’ sex phenotype and performance

Document Type : Research Paper

Authors

1 Graduated from Animal Sciences Department, University of Kurdistan, Sanandaj, Iran

2 Animal Science Department, Faculty of Agriculture

3 Department of Animal Sciences, University of Kurdistan, Sanandaj, Iran

Abstract

This study was conducted to evaluate the effects of an aromatase inhibitors on chick’s sex phenotype in order to produce more male birds which are economically beneficial to the broiler industry. In ovo injection of an aromatase inhibitor was carried out with 360 eggs of Ross 308 that randomly divided into six groups with 5 replicates. Experimental groups included: 50 (L50) and 100 (L100) µg of Letrozole, multivitamin + antibiotic (MA), antibiotic (A), male and female mixed control group (C1), and also male and female separated control group (C2). Hatched chicks were reared with the same diet and condition up to 42 days of age. Percentage of hatchability, hematological parameters, level of steroid hormones, bone strength, and biochemical composition in the progeny broiler chickens were evaluated. In ovo injection of Letrozole did not cause any significant effect on percentage of hatchability and the chicks’ hematological and serum biochemical parameters whereas notably resulted in increased percentage of male phenotype and their serum testosterone level, feed intake, and improved feed covvertion ratio in the broiler chicks (P<0.05). This study reveals that in ovo Letrozole administration results in a higher male phenotype of hatched broiler chicks and markedly improved feed conversion rate of the progenies without negative effects on their survival and hatchability.

Keywords

References

Abinawanto, K., Shimada, K., Yoshida, K., Satio, N. (1996). Effects of aromatase inhibitor on sex differentiation and levels of P450 aromatase and P450 17α messenger ribonucleic acid of gonads in chicken embryos. General and Comparative Endocrinology. 102: 241-246.doi.org/10.1006/gcen.1996.0065.
Brueggemeier, R,W., Hackett, J.C., Diaz-Cruz, E.S. (2005). Aromatase inhibitors in the treatment of breast cancer. Endocrine Reviews. 26: 331-345
Burke, W.H., Henry, M.H. (1999). Gonadal development and growth of chickens and turkeys hatched from eggs injected with an aromatase inhibitor. Poultry Science. 78: 1019-1033. 10.1093/ps/78.7.1019.
Capel, B., Jennifer, B., Jennifer, S. (2000). The battle of the sexes: Sry and the control of Testis organogenesis. Novartis Foundation Symposium. The Genetics and Biology of Sex Determiation. 92: 89-103.
Clinton, M. and McBird, D. (2003). Molecular genetics of sex determination and gonadal development in poultry Somatic sex identity is cell autonomous in the chicken. Nature. 464: 237-242.
 Deng, Y.F., Chen, X.X., Zhou, Z.L., Hou, J.F. (2010). Letrozole inhibits the osteogenesis of medullary bone in prelay pullets. Poultry Science. 89: 917–923. doi.org/10.3382/ps.2010-00632.
 Kavianpoor, M., Zarbakht Ansari, P., Dirandeh, E., Shohre, B. (2019). Effect of in ovo injection of anastrozole on sex ratio and quality of broiler chicks. Research on Animal Production. doi.10.29252/rap.10.24.85.
Lester, J. and Coleman, R. (2005). Bone loss and the aromatase inhibitors. British Journal of Cancer, 93: 16-22.
Mokarrami, T., Navidshad, B., Evrigh, N.H., Aghjegheshlagh, F.M. (2021). The Effect of In Ovo Injection of Fadrozole Hydrochloride, Nettle Extract and Mushroom Extracts on Sex Reversal, Performance Traits, Blood Lipids and White Blood Cells and Muscle Structure of Broiler Chicks. Journal of Veterinary Research: 76: 103-114 (in persian).
Mottaghitalab, M. and Razani, K. (2005). Egg Treatment with Anti-aromatase: Effects on the Chicks Male: Female Ratio, and Their Economic Performance. Iranian Journal of Agricultural Sciences, 36, 375-383 (In Persian).
Nazifi, S. (1995). Hematology and clinical biochemistry of birds. In: Shiraz University Press.  451 (in persian).
Rezaei, A., Vaziry, A., Farshad, A., Farzinpour, A., Rostamzadeh, J. (2020). Effects of letrozole administration on growth and reproductive performance in Markhoz goat bucklings. Theriogenology. 147: 183-191,
Seralini, G.E. and Moslemi, S. (2001). Aromatase inhibitors: Past, Present and future. Molecular and Cellular Endocrinology, 178: 117-131. DOI:10.1016/S0303-7207(01)00433-6.
 Sharp, R.L. and MacLatchy, D.L. (2007). Lipid dynamics in goldfish (Carassius auratus) duringa periodof gonadal recrudescence: Effects of β-sitosterol and 17 β-estradiol exposure. Comparative Biochemistry and Physiology. 145: 507-517. doi.org/10.1016/j.cbpc.2006.11.018.
Shimada, K., Valdezer, M.B., Mizutani, M., Namikawa, T. (2007). Potential application of sperm bearing female specific chromosome in chickens. Cytogenetic and Genome Research, 117: 240-247. doi.org/10.1159/000103185.
Shimada, K. (1998). Gene expression of steroidogenic enzymes in chicken embryonic gonads. Journal of Experimental Zoology, 281, 450–456. PMID: 96628318.
Trukhina, A.V., Lukina, N.A., Wackerov-Kouzova, N.D., Nekrasova, A.A., Smirnov, A.F. (2014). Sex inversion in domestic chicken (Gallus gallus domesticus) by letrozole and tamoxifen. Cell and Tissue Biology. 8: 244–252.
Valizadeh, E. and Seratinouri, H. (2013). Effects of garlic extract, anti-estrogens, and aromatase inhibitor on sex differentiation in embryo. International Journal of Women’s Health and Reproduction Sciences, 1: 51-55. 123456789/53881.
Valizade, A. and Jadiri, H.(2010). Study of the effcet of aromatase inhibitors and anti estrogens on the sex differentiation of broiler chicks. Journal of veterinary diagnostic science, Islamic azad university of Tabriz branch, N 961-964 (In Persian).
Vaziry, A., Karashi, N., Farzinpour, A., Sobhani, K. (2015). Effect of aromatase inhibitor on performance and blood factors in broilers. International Congress of Reproduction, Tehran, Iran (in persian).
Wistedt, A. (2013). Shell formation and bone strength in laying hens: Effect of age, daidzein, and exogenous estrogen. Journal of Acta Universitatis agriculturae Sueciae. 88: 1-73. 1652-6880.
Yang, X. and Zheng, J. (2008). Degree of sex differentiation of genetic female chicken treated with different doses of an aromatase inhibitor. Journal of Sexual Development. 2: 309-315. doi: 10.1159/000195680.
Zandi, N., Farzinpour, A., Vaziry, A. (2019). Letrozole administration as a new way of regulating reproductive activity in female quail. Journal of Applied Poultry Research. 28: 4. 1288-1296.
Animal Sciences Journal
Volume 35, Issue 136
December 2022
Pages 45-58

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