Document Type : Research Paper

Authors

1 Department of Animal Science, College of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

2 Animal science, animal science and fisheries faculty, sari agricultural and natural resources university. Sari, Iran

Abstract

This experiment was conducted to investigate the effect of adding enzymes and probiotics in diets containing different levels of metabolizable energy on performance, carcass characteristics, intestinal morphology and microbial population in broiler chickens fed with a diet based on wheat-soybean meal. This experiment was performed with 8 treatments and 5 replications with 400 maleRoss 308 broiler chicks. Treatments including diets with and without multi-enzyme (0, 500 g/t), with and without probiotics lactofid (0, 200 g/t) and two levels of metabolizable energy (2850 and 3100 kcal/kg) in a 2×2×2 factorial arrangement with completely randomized design was. Adding ration energy to 3100 kcal/kg resulted in improvement of feed consumption and weight gain in the whole experiment period (P<0.05). Addition of probiotic and multienzyme increased villi height and width, decreased crypt depth and increased crypt height-to-depth ratio in duodenum of 24-day-old chickens (P<0.05). The addition of probiotics increased the bacterial population of Lactobacillus and decreased the coliform (P<0.05). Adding the energy level of the diet to 3100 kcal/kg increased the population of bacteria (lactobacillus, coliform and total bacteria) (P<0.05), but the interaction effect of multienzyme, probiotic and energy on the microbial population of the digestive tract did not have a significant effect (P>0.05). In general, adding enzymes and probiotics to the diet with 3100 kcal of metabolizable energy increased feed consumption and improved the beneficial microbial population.

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