Document Type : Research Paper
Author
Institute of Zabol
Abstract
This study aimed to investigate the effects of different levels of synbiotic on performance, intestinal microbial population and morphology of broiler chicks subjected to chronic heat stress and estimation of optimal point of synbiotic using broken line modeling. In total, 240 one-day-old (Ross 308) broiler chicks were allocated into four experimental treatments with five replications (12 chicks) in complete random design. Treatments containing 0, 0.5, 1.0 and 1.5 (g/Kg) synbiotic added to experimental diet from 3 to 42 days. Chronic heat stress was induced from 28 to 42 days of age to all groups (32 degree Celsius for 6 hours, from 10:00 am to 4:00 pm).The results showed that synbiotic improved body weight gain and feed intake significantly to 28 day and during chronic heat stress (p≥0.05). So that, the groups receiving 1 and 1.5 (g/kg) of synbiotic had the highest performance. Treatments affected microbial population and caused increasing lactic-acid producing bacteria (p≥0.05). Moreover, the result showed that treatments made significant difference in intestinal morphology and increased villus height (p≥0.05). European production index significantly increased the groups receiving 1 and 1.5 (g/kg) of synbiotic (p≥0.05). The results of this study showed that using synbiotic during chronic heat stress, improved performance through positive changes in morphology and microbial population of intestine. Based on linear and quadratic broken line modeling, the optimal point of synbiotic are 1.3 and 1.42 (g/Kg diet) respectively.
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Main Subjects
Abd Al-Fatah, M. (2020). Probiotic modes of action and its effect on biochemical parameters and growth performance in poultry. Iranian Journal of Applied Animal Science. 10 (1): 9-15.
Adil, S., and Magray, S. N. (2012). Impact and manipulation of gut microflora in poultry: a review. Journal of Animal and Veterinary Advances.1 (6):73-877. DOI: 10.3923/javaa.2012.873.877
Akbarian, A., Golian, A.H., Kermanshahi, R, Farhoosh, A.R., Raji, S. (2013). Growth performance and gut health parameters of finishing broilers supplemented with plant extracts and exposed to daily increased temperature. Spanish Journal Agricalture Research. 11:109-119. DOI: 10.5424/sjar/2013111-3392.
Ashraf, H., Zaneb, M.S., Yousaf, A., Ijaz, M.U., Sohail, S., Muti, S., Usman, M.M., Ijaz, S. and Rehman, H. (2013). Effect of dietary supplementation of prebiotics and probiotics on intestinal microarchitecture in broilers reared under cyclic heat stress. Journal Animal of Physiology and Nutrition. 97: 68-73. DOI:10.1111/jpn.12041.
Chen, C., Zhang, H., Xie, Y., Xiong, L., Liu, H. and Wang, F. (2020). Effects of a probiotic on the growth performance, intestinal flora, and immune function of chicks infected with Salmonella pullorum. Poultry Science. 99(11): 5316-5323. DOI: 10.1016/j.psj.2020.07.017.
Gasparino, E., Del Vesco, A. P., Voltolini, D. M., Do Nascimento, C. S., Batista, E., Gasparino, E. et al. (2014). The effect of heat stress on GHR, IGF-I, ANT, UCP and COXIII mRNA expression in the liver and muscle of high and low feed efficiency female quail. British Poultry Science. 55 (4): 466-473. DOI: 10.1080/00071668.2014.925090.
Humam, A. M., Loh, T. C., Foo, H. L., Samsudin, A. A., Mustapha, N. M., Zulkifli, I., et al. (2019). Effects of feeding different postbiotics produced by Lactobacillus plantarum on growth performance, carcass yield, intestinal morphology, gut microbiota composition, immune status, and growth gene expression in broilers under heat stress. Animals. 9 (9): 644-660. DOI:10.3390/ani9090644
Jayaraman, S., Das, P. P., Saini, P. C., Roy, B., and Chatterjee, P. N. (2017). Use of Bacillus Subtilis PB6 as a potential antibiotic growth promoter replacement in improving performance of broiler birds. Poultry Science. 96(8): 2614-2622. DOI: 10.3382/ps/pex079.
Jin, L. Z., Y. W. Ho, N. Abdullah, and Jalaludin, S. 1997. Probiotics in poultry: Modes of action. Worlds Poultry Science Journal. 53: 351-368. DOI:10.1079/WPS19970028
Khomayezi, R and Adewole, D. (2022) Probiotics, prebiotics, and synbiotics: an overview of their delivery routes and effects on growth and health of broiler chickens, World's Poultry Science Journal, 78:1, 57-81, DOI: 10.1080/00439339.2022.1988804.
Landy, N., and Kavyani, A. (2013). Effects of using a multi‐strain probiotic on performance, immune responses and cecal microflora composition in broiler chickens reared under cyclic heat stress condition. Iranian Journal of Applied Animal Science. 3(4): 703-708.
Liu, L., Fu, C., Yan, M., Xie, H., Li, S., Yu, Q., et al. (2016). Resveratrol modulates intestinal morphology and HSP70/90, NF-κB and EGF expression in the jejunal mucosa of black-boned chickens on exposure to circular heat stress. Food and Function. 7(3): 1329-1338. DOI:10.1039/C5FO01338K.
Markazi, A., Luoma, A., Shanmugasundaram, R., Mohnl, M., Murugesan, G. R., and Selvaraj, R. (2018). Effects of drinking water synbiotic supplementation in laying hens challenged with Salmonella. Poultry Science. 97(10): 3510-3518. DOI:10.3382/ps/pey234.
Mohammed, A. A., Jiang, S., Jacobs, J. A., and Cheng, H. W. (2019). Effect of a synbiotic supplement on cecal microbial ecology, antioxidant status, and immune response of broiler. chickens reared under heat stress. Poultry Science, 98(10): 4408-4415. DOI:10.3382/PS/PEZ246.
Mohammed, Hu, J. Y., Murugesan, A. A., and Cheng, H. W. (2022). Effect of a synbiotic supplement as an antibiotic alternative on broiler skeletal, physiological, and oxidative parameters under heat stress. Poultry Science.101(4):101-112. DOI: 10.1016/j.psj.2022.101769.
Mora, Z., Nuño, K., Vázquez-Paulino, O., Avalos, H., Castro-Rosas, J., Gómez-Aldapa, et al. (2019). Effect of a synbiotic mix on intestinal structural changes, and Salmonella Typhimurium and Clostridium perfringens colonization in broiler chickens. Animals. 9(10): 777. Doi: 10.3390/Ani9100777.
Murugesan, G. R., and M. E. Persia. (2015). Influence of a direct- fed microbial and xylanase enzyme on the dietary energy uptake efficiency and performance of broiler chickens. Journal of Science Food and Agriculture. 95:2521-2527. DOI:10.1002/JSFA.6984.
Naghi Shokri, A., Ghasemi, H. A., and Taherpour, K. (2017). Evaluation of Aloe vera and synbiotic as antibiotic growth promoter substitutions on performance, gut morphology, immune responses and blood constitutes of broiler chickens. Animal Science Journal. 88(2): 306-313. DOI: 10.1111/ASJ.12629.
Pesti, G. M., J. A. Vedenov, J. A. Cason, and Billard, L. (2009). A comparison of methods to estimate nutritional requirements from experimental data. Poultry Science. 50: 16-320. DOI: 10.1080/00071660802530639.
Renaudeau, D., Collin, A., Yahav, S., de Basilio, V., Gourdine, J. L., and Collier, R. J. (2012). Adaptation to hot climate and strategies to alleviate heat stress in livestock production. Animal. 6(05): 707-728. DOI: 10.1017/S1751731111002448 .
Sejian, V., Bhatta, R., Gaughan, J. B., Dunshea, F. R., and Lacetera, N. (2018). Adaptation of animals to heat stress. Animal. 12(s2): s431-s444. DOI:10.1017/S1751731118001945 .
Sohail, M. U., Rahman, Z. U., Ijaz, A., Yousaf, M. S., Ashraf, K., Yaqub, T., et al. (2011). Single or combined effects of mannan-oligosaccharides and probiotic supplements on the total oxidants, total antioxidants, enzymatic antioxidants, liver enzymes, and serum trace minerals in cyclic heat-stressed broilers. Poultry Science. 90(11): 2573-2577. doi:10.3382/ps.2011-01502
Sohail, M. U., Ijaz, A., Younus, M., Shabbir, M. Z., Kamran, Z., Ahmad, S. and Rehman, H. (2013). Effect of supplementation of mannan oligosaccharide and probiotic on growth performance, relative weights of viscera, and population of selected intestinal bacteria in cyclic heat-stressed broilers. Journal of Applied Poultry Research. 22(3): 485-491. DOI: 10.3382/JAPR.2012-00682.
Hassan, A. M., M. H. Abd ELAz, M. M. Hussein, M. M. Osman, and Z. H. Abd El-Wahed. (2007). Effect of chronic heat stress on broiler chicks’ performance and immune system. SCVMJ. 12:55-68.
Thompson, K. L., and Applegate, T. J. (2006). Feed withdrawal alters small-intestinal morphology and mucus of broilers. Poultry Science. 85:1535-1540. DOI: 10.1093/PS/85.9.1535.
Wu, Q. J., Liu, N., Wu, X. H., Wang, G. Y., and Lin, L. (2018). Glutamine alleviates heat stress-induced impairment of intestinal morphology, intestinal inflammatory response, and barrier integrity in broilers. Poultry Science. 97(8): 2675-2683. DOI: 10.3382/PS/PEY123.
Yahav, S. (2009). Alleviating heat stress in domestic fowl: different strategies. World’s Poultry Science Journal. 44(32): 719–732. DOI:10.1017/S1751731111002448.
Yan, F., Wang, W., Wolfenden, R., and Cheng, H. (2016). The effect of bacillus subtilis based probiotic on bone health in broiler chickens. Poultry Science. 223: 95-40. DOI: 10.1016/J.PSJ.2020.11.073.
Zaboli, G. R., Rahimi, S., Shariatmadari, F., Torshizi, M. A. K., Baghbanzadeh, A., and Mehri, M. (2017). Thermal manipulation during Pre and Post-Hatch on thermotolerance of male broiler chickens exposed to chronic heat stress. Poultry Science. 96(2): 478-485. DOI:10.3382/PS/PEW344.
Zaboli, G., Huang, X., Feng, X., and Ahn, D. U. (2019). How can heat stress affect chicken meat quality? a review. Poultry Science. 98(3): 1551-1556. DOI: 10.3382/PS/PEY399.
Zhang, C., Zhao, X. H., Yang, L., Chen, X. Y., Jiang, R. S., Jin, S. H., and Geng, Z. Y. (2017). Resveratrol alleviates heat stress-induced impairment of intestinal morphology, microflora, and barrier integrity in broilers. Poultry Science. 96(12): 4325-4332. DOI:10.3382/PS/PEX266.
Zhang, P., T. Yan, X. Wang, S. Kuang, Y. Xiao, W. Lu, and D. Bi. (2017). Probiotic mixture ameliorates heat stress of laying hens by enhancing intestinal barrier function and improving gut microbiota. Italian Journal Animal Science. 16:292–300. DOI:10.1080/1828051X.2016.1264261.
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