Document Type : Research Paper
Authors
1 Animal Science Department, Faculty of Agriculture Science, University of Yasouj, Yasouj, Iran
2 Department of Animal Sciences faculty of Agriculture Yasouj University yasouj iran
Abstract
The effect of mRNA levels in tyrosinase family genes (TYR, TYRP1 and DCT) and MATP gene on the development of skin spots were investigated in Lori Bakhtiari sheep. For this porpose, the skin samples were collected from both white and dark parts of 14 white-haired Lori-Bakhtiari sheep with black spots and total RNA was extracted. In addition, after determining the quality and quantity of extracted RNA, cDNA was synthesized for each sample. To amplify fragments of the target and reference genes, the necessary primers were designed based on the mRNA sequences available in GenBank and Primer3plus software. The result showed mRNA levels of DCT and MATP genes were not significantly different in spotted skin tissue compared to white skin tissue. compared to the white part of the skin tissue. Among the target genes, the DCT gene with an average threshold cycle (Ct) of 35.78 had a lower expression level than the MATP gene with an average Ct of 31.68 in skin samples of Lori-Bakhtiari sheep. The expression of TYR and TYRP1 genes were not detected in any of the samples from both parts of the skin. The results of this study showed that the expression level of DCT, TYR, TYRP1 and MATP genes can not play an effective role in regulating the coat color and creating black spots in the skin tissue of Lori Bakhtiari sheep. Therefore, it appears that genes or other factors cause black spots in Lori-Bakhtiari sheep, and their identification requires further study.
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