The effect of adding Mito-TEMPO to soybean lecithin-based extender on cooling and freezing ability of buck semen

Zarei, Fatemeh; Asadzadeh, Nader; Daghighkia, H.; Gholami Karkan, Ali; Azizi, Mohammad; Heidari, Mohammad; Khorrami, Shahrouz

doi:10.22092/asj.2024.367520.2430

Document Type : Research Paper

Authors

¹ Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran

² Assistant professor of Animal Science Research Institute of Iran

³ Department of Animal Science, College of Agriculture, Tabriz University, Tabriz, Iran

⁴ Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.

https://doi.org/10.22092/asj.2024.367520.2430

Abstract

The current study was aimed to investigate the effect of adding different concentrations of mitochondria-targeted antioxidant Mito-TEMPO to soybean lecithin-based extender on the cooling and freezing ability of buck semen. For this purpose, five adult Saanen bucks were used. After semen collection by using an artificial vagina and primary evaluation, semen samples were pooled in order to eliminate individual differences. In experiment 1, semen samples were added to extenders containing 0, 1, 10, 100 and 1000 μM Mito-TEMPO. The prepared samples were stored at 4 ◦C up to 60 h. Chilled sperm motility, progressive motility, viability and membrane functionality were analyzed during 0, 30 and 60 h. In experiment 2, semen was added to the extenders containing 0, 1, 10, 100 and 1000 μM Mito-TEMPO and were frozen. Microscopic evaluation of spermatozoa was done after freeze-thawing and quality parameters including motion parameters, abnormal morphology, membrane integrity and acrosome integrity were assessed. The results of the cooling process showed that at time 0, no difference was observed among groups but at 30 and 60 h storage and after the freezing process, higher (P ≤ 0.05) sperm total motility, progressive motility, membrane functionality and viability were observed in 10 and 100 μM Mito-TEMPO groups than the others. In conclusion, supplementation of buck cooling and freezing medium with Mito-TEMPO as a novel mitochondria-targeted antioxidant could be an impressive method to conserve of buck's semen quality during the storage process.

Keywords

Main Subjects

Animal and poultry physiology

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Research Journal of Livestock Science

The effect of adding Mito-TEMPO to soybean lecithin-based extender on cooling and freezing ability of buck semen

References

References

Volume 38, Issue 148
December 2025
Pages 175-184

The effect of adding Mito-TEMPO to soybean lecithin-based extender on cooling and freezing ability of buck semen

References

References

Volume 38, Issue 148December 2025Pages 175-184

Volume 38, Issue 148
December 2025
Pages 175-184