Document Type : Research Paper

Authors

1 MSc in Animal and Poultry Nutrition, Gorgan university of Agricultural Sciences and Natural Resources

2 Assistant Professor, Department of Animal and Poultry Nutrition, Gorgan university of Agricultural Sciences and Natural Resources (Corresponding Author)

3 Associate Professor, Department of Animal and Poultry Nutrition, Gorgan university of Agricultural Sciences and Natural Resources

4 Associate Professor, Department of Animal and Poultry Physiology, Breeding and Genetics, Gorgan university of Agricultural Sciences and Natural Resources

Abstract

In the present study the effect of flaxseed oil feeding duration on growth performance, abdominal fat, selected blood parameters, and duodenal morphology of Japanese quails was evaluated. A total of 540 Japanese quails (mixed sex) were allocated in a completely randomized design with six treatments, six replicates, and 15 birds per replicate. The treatments were designed based on the weekly replacement of sunflower oil with flaxseed oil in the diet of Japanese quails. The results showed that the duration of flaxseed oil feeding had no significant effect on body weight. The highest average feed intake was observed in quails fed flaxseed oil from days 15–35. The best feed conversion ratio was recorded in the group fed flaxseed oil from days 29–35. Flaxseed oil feeding and increasing the feeding duration significantly reduced abdominal fat deposition. Blood levels of glucose, total protein, globulin, and uric acid were significantly influenced by dietary treatments, whereas albumin concentration showed no significant differences among treatments. Flaxseed oil feeding increased duodenal villus width, although villus absorptive surface area was not affected by treatments. Overall, the results indicated that replacing sunflower oil with flaxseed oil in Japanese quail diets improved feed conversion ratio (particularly in the late growth phase) and reduced abdominal fat deposition, regulated blood metabolic parameters. Induced favorable modifications in intestinal morphology (increased villus width), although without altering the absorptive surface area. These findings suggest that flaxseed oil has potential to improve production efficiency and bird health.

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روحی، ز.، مقصودلو، ش.، تراز، ز و قنبری، ف. (1401). اثر رژیم های مختلف پروتئین جیره بر عملکرد، خصوصیات لاشه و صفات اقتصادی بلدرچین‌های ژاپنی درحال رشد. پژوهش‌های تولیدات دامی، 13(35)، 1-11.‎
Attia, Y., El‐kelawy, M., Al‐harthi, M., & El‐shafey, A. (2020). Impact of multienzymes dose supplemented continuously or intermittently in drinking water on growth performance, nutrient digestibility, and blood constituents of broiler chickens. Animals, 10(3). https://doi.org/10.3390/ani10030375
Awad, W., Ghareeb, K., & Böhm, J. (2008). Intestinal structure and function of broiler chickens on diets supplemented with a synbiotic containing Enterococcus faecium and oligosaccharides. International Journal of Molecular Sciences, 9(11), 2205–2216. https://doi.org/10.3390/ijms9112205
Azcona, J. O. J. O. ., Schang, M. J. . M. J., Garcia, P. T. . P. T., Gallinger, C. C. ., Jr, R. A., Coates, W. . W., … Coates, W. . W. (2008). Omega-3 enriched broiler meat : The influence of dietary a -linolenic- v -3 fatty acid sources on growth , performance and meat fatty acid composition. Canadian Journal of Animal Science, 88(2), 257–269. https://doi.org/10.4141/CJAS07081
Campioli, E., Rustichelli, C., & Avallone, R. (2012). N-3 Dietary supplementation and lipid metabolism: Differences between vegetable- and fish-derived oils. Journal of Functional Foods, 4(1), 207–212. https://doi.org/10.1016/j.jff.2011.10.006
Chaves, H., Singh, R. B., Khan, S., Wilczynska, A., & Takahashi, T. (2019). High Omega-6/Omega-3 Fatty Acid Ratio Diets and Risk of Noncommunicable Diseases. The Role of Functional Food Security in Global Health, 217–259. https://doi.org/10.1016/B978-0-12-813148-0.00014-1
Choi, A. M., Lee, S. B., Cho, S. H., Hwang, I., Hur, C. G., & Suh, M. C. (2008). Isolation and characterization of multiple abundant lipid transfer protein isoforms in developing sesame (Sesamum indicum L.) seeds. Plant Physiology and Biochemistry, 46(2), 127–139. https://doi.org/10.1016/j.plaphy.2007.10.003
Cortinas, L., Villaverde, C., Galobart, J., Baucells, M. D., Codony, R., & Barroeta, A. C. (2004). Fatty Acid Content in Chicken Thigh and Breast as Affected by Dietary Polyunsaturation Level. Poultry Science, 83(7), 1155–1164. https://doi.org/10.1093/ps/83.7.1155
Crespo, N., & Esteve-Garcia, E. (2001). Dietary fatty acid profile modifies abdominal fat deposition in broiler chickens. Poultry Science, 80, 71–78. https://doi.org/10.1093/ps/80.1.71
Crespo, N., & Esteve-Garcia, E. (2002). Nutrient and fatty acid deposition in broilers fed different dietary fatty acid profiles. Poultry Science, 81(10), 1533–1542. https://doi.org/10.1093/ps/81.10.1533
Crespo, N., & Esteve-Garcia, E. (2003). Polyunsaturated fatty acids reduce insulin and very low density lipoprotein levels in broiler chickens. Poultry Science, 82(7), 1134–1139. https://doi.org/10.1093/ps/82.7.1134
Davis, J. E., Cain, J., Small, C., & Hales, D. B. (2016). Therapeutic effect of flax-based diets on fatty liver in aged laying hens. Poultry Science, 95(11), 2624–2632. https://doi.org/10.3382/ps/pew160
Emmans, G. C. (1994). Effective energy: a concept of energy utilization applied across species. British Journal of Nutrition, 71(6), 801–821. https://doi.org/10.1079/bjn19940188
Fébel, H., Mézes, M., Pálfy, T., Hermán, A., Gundel, J., Lugasi, A., … Blázovics, A. (2008). Effect of dietary fatty acid pattern on growth, body fat composition and antioxidant parameters in broilers. Journal of Animal Physiology and Animal Nutrition, 92(3), 369–376. https://doi.org/10.1111/j.1439-0396.2008.00803.x
Fries-Craft, K. A., Meyer, M. M., Lindblom, S. C., Kerr, B. J., & Bobeck, E. A. (2021). Lipid Source and Peroxidation Status Alter Immune Cell Recruitment in Broiler Chicken Ileum. Journal of Nutrition, 151(1), 223–234. https://doi.org/10.1093/jn/nxaa356
Fu, Y., Wang, Y., Gao, H., Li, D., Jiang, R., Ge, L., … Xu, K. (2021). Associations among Dietary Omega-3 Polyunsaturated Fatty Acids, the Gut Microbiota, and Intestinal Immunity. Mediators of Inflammation, 2021. https://doi.org/10.1155/2021/8879227
Hsu, J. M., & Ding, S. T. (2003). Effect of polyunsaturated fatty acids on the expression of transcription factor adipocyte determination and differentiation-dependent factor 1 and of lipogenic and fatty acid oxidation enzymes in porcine differentiating adipocytes. British Journal of Nutrition, 90(3), 507–513. https://doi.org/10.1079/bjn2003918
Hwang, D. (2000). Fatty acids and immune responses: a new perspective in searching for clues to mechanism. Annual Review of Nutrition, 20, 431.
Kanakri, K., Carragher, J., Hughes, R., Muhlhausler, B., & Gibson, R. (2017). A reduced cost strategy for enriching chicken meat with omega-3 long chain polyunsaturated fatty acids using dietary flaxseed oil. British Poultry Science, 58(3), 283–289. https://doi.org/10.1080/00071668.2017.1293798
Kartikasari, L. R. (2009). Assessment of Omega-3 Long Chain Polyunsaturated Fatty Acid Incorporation in Broiler Chicken Meat Following the Consumption of Omega-3 Rich Vegetable Oils. Sciences-New York, (February).
Khalid, K., Khalil, I., Islam, A., Sujan, K. M., Mustari, A., & Ahmad, N. (2020). Dietary acidifier and lysozyme improve growth performances and hemato-biochemical profile in broiler chicken. (July). https://doi.org/10.5455/jabet.2020.d130
Khatun, J., Loh, T. C., Akit, H., Foo, H. L., & Mohamad, R. (2017). Influence of different sources of oil on performance, meat quality, gut morphology, ileal digestibility and serum lipid profile in broilers. Journal of Applied Animal Research, 2119, 1–7. https://doi.org/10.1080/09712119.2017.1337580
Kolmstetter, C. M., & Ramsay, E. C. (2000). Effects of feeding on plasma uric acid and urea concentrations in Blackfooted Penguins (Spheniscus demersus). J Avian Med Surg 2000, 14, 177–179.
Lee, S. H., Kim, Y. B., Kim, D. H., Lee, D. W., Lee, H. G., Jha, R., & Lee, K. W. (2021). Dietary soluble flaxseed oils as a source of omega-3 polyunsaturated fatty acids for laying hens. Poultry Science, 100(8). https://doi.org/10.1016/j.psj.2021.101276
Limdi, J. K., & Hyde, G. M. (2003). Evaluation of abnormal liver function tests. Postgrad Med J, 79, 307–312.
López-Ferrer, S., Baucells, M. D., Barroeta, A. C., & Grashorn, M. A. (1999). N-3 Enrichment of Chicken Meat Using Fish Oil: Alternative Substitution With Rapeseed and Linseed Oils. Poultry Science, 78(3), 356–365. https://doi.org/10.1093/ps/78.3.356
Lopez-Ferrer, S., Baucells, M. D., Barroeta, A. C., Grashorn, M. A., Galobart, J., & Grashorn, M. A. (2001). n-3 Enrichment of Chicken Meat. 1. Use of Very Long-Chain Fatty Acids in Chicken Diets and Their Influence on Meat Quality: Fish Oil. Poultry Science, 80(6), 741–752. https://doi.org/10.1093/ps/80.6.741
Mirshekar, R., Dastar, B., & Shams Shargh, M. (2021). Supplementing flaxseed oil for long periods improved carcass quality and breast fatty acid profile in Japanese quail. Animal, 15(2). https://doi.org/10.1016/j.animal.2020.100104
Montagne, L., Pluske, J. R., & Hampson, D. J. (2021). A review of interactions between dietary fibre and the intestinal mucosa, and their consequences on digestive health in pigs. Anim Nutr, 7(4), 989–1003. Retrieved from https://doi.org/10.1016/j.aninu.2021.07.003
Mridula, D., Kaur, D., Nagra, S.S., Barnwal, P., Gurumayum, S. and Singh, K.K. (2015). Growth performance and quality characteristics of flaxseed-fed broiler chicks. Journal of Applied Animal Research, 43(3), pp.345-351.
Newman, R. E., Bryden, W. L., Kirby, A. C., Storlien, L. H., & Downing, J. A. (2005). Dietary n-3 and n-6 fatty acids alter avian glucose metabolism. British Poultry Science, 46(1), 104–113. https://doi.org/10.1080/00071660400023987
NRC. (1994). Nutrient Requirements of Poultry. In Nutrient Requirements of Poultry (9th, rev, ed.). https://doi.org/10.17226/2114
Pelicano, E. R. L., Souza, P. A., Souza, H. B. A., Figueiredo, D. F., Boiago, M. M., Carvalho, S. R., & Bordon, V. F. (2005). Intestinal mucosa development in broiler chickens fed natural growth promoters. Revista Brasileira de Ciência Avícola, 7, 221–229.
Pesti, G. M., Bakalli, R. I., Qiao, M., & Sterling, K. G. (2002). A comparison of eight grades of fat as broiler feed ingredients. Poultry Science, 81(3), 382–390. https://doi.org/10.1093/ps/81.3.382
Popescu, R. G., Voicu, S. N., Gradisteanu Pircalabioru, G., Gharbia, S., Hermenean, A., Georgescu, S. E., … Dinischiotu, A. (2021). Impact of dietary supplementation of flaxseed meal on intestinal morphology, specific enzymatic activity, and cecal microbiome in broiler chickens. Applied Sciences (Switzerland), 11(15). https://doi.org/10.3390/app11156714
Riccardi, G., Giacco, R., & Rivellese, A. A. (2004). Dietary fat, insulin sensitivity and the metabolic syndrome. Clinical Nutrition, 23(4), 447–456. https://doi.org/10.1016/j.clnu.2004.02.006
Sagher, F. A., Dodge, J. A., Johnston, C. F., Shaw, C., Buchanan, K. D., & Carr, K. E. (1991). Rat small intestinal morphology and tissue regulatory peptides: effects of high dietary fat. British Journal of Nutrition, 65(1), 21–28. https://doi.org/10.1079/bjn19910062
Sampath, H., & Ntambi, J. M. (2007). Polyunsaturated fatty acids and regulation of gene expression. Fatty Acids in Foods and Their Health Implications, Third Edition, 727–739. https://doi.org/10.1111/j.1753-4887.2004.tb00058.x
Sanz, M., Flores, A., & Carmona, J. M. (2000a). Effect of the Inclusion Time of Dietary Saturated and Unsaturated Fats Before Slaughter on the Accumulation and Composition of Abdominal Fat in Female Broiler Chickens. Chemical Analysis, 1320–1325.
Sanz, M., Lopez-Bote, C. J., Menoyo, D., & Bautista, J. M. (2000b). Abdominal Fat Deposition and Fatty Acid Synthesis Are Lower and {beta}-Oxidation Is Higher in Broiler Chickens Fed Diets Containing Unsaturated Rather than Saturated Fat. J. Nutr., 130(12), 3034–3037. Retrieved from http://jn.nutrition.org/cgi/content/abstract/130/12/3034
Tandoğan, M. and Çiçek, H., 2016. Technical performance and cost analysis of broiler production in Turkey. Revista Brasileira de Ciência Avícola, 18(1), pp.169-174.
Thomson, A. B., Keelan, M., Garg, M., & CLAndinin, M. T. (1987). Spectrum of effects of dietary long chain fatty acids on rat intestinal glucose and lipid uptake. Can. J. Physiol. Pharmacol, 65, 2459–2465.
Valavan, S. E., Selvaraj, P., Mohan, B., Sundaram, T. K., & Viswanathan, K. (1990). Effects of various n-3 lipid sources on the quality characteristics and fatty acids composition of chicken meat.
Velasco, S., Ortiz, L. T., Alzueta, C., Rebolé, A., Treviño, J., & Rodríguez, M. L. (2010). Effect of inulin supplementation and dietary fat source on performance, blood serum metabolites, liver lipids, abdominal fat deposition, and tissue fatty acid composition in broiler chickens. Poultry Science, 89(8), 1651–1662. https://doi.org/10.3382/ps.2010-00687