Detection of polymorphism in FecB and BMP15 candidate genes associated with litter size in Moghani flock

Savar Sofla, S.; Seyedabadi, H. R.; Javanrouh Aliabad, A.

doi:10.22092/asj.2015.101350

Document Type : Research Paper

Authors

https://doi.org/10.22092/asj.2015.101350

Abstract

Genetic variation in ovulation rate in sheep has been widely documented and the evidence shows substantial differences among world sheep breeds. Candidate gene approach and DNA marker strategy are new tools to improve twining or multiple lambing rate traits in sheep population .The present study was carried out to detect of polymorphism in FecB and BMP15 genes and the effect on litter size trait in Moghani sheep breed using RFLP-PCR method. Blood samples were collected randomly from 150 individuals at Moghani Breeding Station of Ardebil province, Iran. DNA of blood samples was extracted by modifying salting out method. Site of mutation was amplified using specific primers and PCR products were determined by agarose gel electrophoresis, and then the PCR product was digested with AvaII, SpeI and HinfI restriction enzymes for FecB, FecX^H and FecX^G loci , respectively. The results did not show any polymorphism for FecB and BMP15 genes. All the samples were showed wild type alleles so mutation of FecB and BMP15 genes were not cause of prolificacy in Moghani sheep. Considering the phenotypes records in this breed, the obtained result indicates that the genetic factor responsible for twining or multiple lambing rate is not related to reported mutated alleles at prolificacy major genes and therefore other prolificacy related genes should be investigated in Moghani sheep.

Keywords

References

Borni, J ., Bedhiaf, S and Djemali, M., 2011. Study for identification FecXI and FecXH mutations inTunisian Barbarine sheep. roavs, 1(2),pp: 112-115.

Chu, M. X., C. L. Jiao, Y. Q. He, J. Y. Wang, Z. H. Liu and G. H. Chen. 2007. Association between PCR-SSCP of bone morphogenetic protein 15 gene and prolificacy in Jining Grey goats. Anim. Biotechnol. 18, pp:263-274.

Davis, G. H., Susan, M. G., Ross, I. K., Gregan, S. M., Ward, J., Nimbkar, B. v., Ghalsasi, P.M., Nimbkar, C., Gray, G. D., Subandriyo, Inounu, I., Tiesnamurti, B., Martyniuk, E., Eythorsdottir, E., Mulsant, P., Lecerf, E, Hanrahan, J. P., Bradford, G. E., and T. Wilson. 2002. DNA test in prolific sheep from eight countries provide new evidence on origin of the Booroola (FecB) mutation. J. Biology of Reproduction. 66, pp:1869-74.

Galloway, S.M., Gregan, S.M., Wilson, T., McNatty, K.P., Jungel, J.L., Ritvos, O., Daivis, G.H. 2002. BMP15 mutations and ovarian function. Molecular Cell Endocrinology. 191:15-18.

Ghaffari , M., Nejati, A., Rahimi, G. 2009.Detection of Polymorphism in BMPR-IB Gene Associated with Twining in Shal Sheep using PCR-RFLP Method. Int. J. Agric. Biol., 11, pp: 97–99

Gursel , F.E., Akis, I., Durak, H., Mengi, A. 2011. Determination of BMP-15, BMPR1B and GDF-9 Gene Mutations of the Indigenous Sheep Breeds in Turkey. Kafkas univ vet fak derg. 17(5). pp:725-729.

Guan, F., Liu, S., Shi, G.Q., Ai, J.T., Mao, D.G and Yang, L.G. 2006. Polymorphism of FecB gene in nine sheep breeds or strains and its effects on litter size, lamb growth and development. Yi Chuan Xue Bao. 33(2). pp:117-24.

Hua Hua, G., Chen, S.L., Ai , J.T. 2008. None of polymorphism of ovine fecundity major genes FecB and FecX was tested in goat. Animal Reproduction Science. 108, pp: 279–286.

Jamshidi, R., Kasiryan, M.M., Hafezeyan, H. 2009. Application of PCR-RFLP technique to determine BMP15 gene polymorphism in Sangsari sheep bred of Iran.Journal of Animal and Veterinary Advances. 8(10), pp: 1906-1910.

Javanmard, A., Azadzadeh, N., Esmailizadeh, A.K. 2011. Mutations in bone morphogenetic protein 15 and growth differentiation factor 9 genes are associated with increased litter size in fat-tailed sheep breeds. Vet Res Commun. 35(3), pp:157-67.

Javanrouh, A., Banabais, M.H., Esmaeilkhanian, S., Amirinia, C., Seyedabadi, H.R.,& Emrani, H. 2006. Optimization onsalting out method for DNA extraction from animal and poultry blood cells. The 57th Annual Meeting of the European Association for Animal Production. Antalya, Turkey.

Kumar, S., Kolte, A.P., Mishra, A.K., Arora, A.L., Singh, V.K. 2006. Identiﬁcation of the FecB mutation in Garole × Malpura sheep and its effect on litter size. Small Rumin. Res. 64, pp: 305–310.

Malher, X., Chere, A.K.1998. High proliﬁcacy in Belle-Ile sheep (Brittany, France): major eﬀects of a putative single gene and the A wh colour gene on ovulation rate and litter size, Reprod. Nut. Dev. 38, pp:473–484.

McNatty, K.P., Juengel, J.L., Reader, K.L., Lun, S., Myllymaa, S., Lawrence, S.B. 2005. Bone morphogenetic protein 15 and growth differentiation factor 9 cooperate to regulate granulosa cell function in ruminants. Reproduction. 129, pp:481–487.

Mulsant, P., Lecerf, F., Fabre, S., Schibler, L., Monget, P., Lanneluc, I., Pisselet, C., Riquet, J., Monniaux, D., Callebaut, I., Cribiu, E., Thimonier, J., Teyssier, J., Bodin, L., Cognie, Y., Elsen, J.M. 2001.Mutation in bone morphogenetic protein receptor-1B is associated with increased ovulation rate in Booroola Merino ewes, Proc. Natl. Acad. Sci. USA 98,pp: 5104–5109.

Piper, L,R., Bindon, B.M and Davis, G.H. 1985. The single gene inheritance of the high litter size of the Booroola Merino. In Genetics of Reproduction in Sheep, pp :115–125.

Souza, C. J. H., MacDougall, c., Cambell, B. K., McNeilly, A. S., and Baird, D. T. 2001. The Booroola (FecBOphenotype is associated with a mutation in the bone morphogenetic receptor type B (BMPR)B) gene. J. Endocri. 69, pp:RI-R6.

Wilson, T., Xiyang W., Juengel, J. L., Ross, I. K., Lumsden, J. M., Lord, E. A., Dodds, K. G., walling, G. A., McEwan, J. C., O'Connell, A. R., McNatty, K. P.,and Montgomery, G. W. 2001. Highly prolific Booroola sheep have a mutation in the intracellular kinase domain of bone morphogenetic protein IB receptor (ALK-6) that is expressed in both oocytes and granulosa cells. J. BioI. of Repro. 64, pp:1225-35.

Yeh, F.C., Yang, R., Boyle T. 1999. POPGENE. Version 1.31. Microsoft Window–based Freeware for Population Genetic Analysis, University of Alberta. Edmonton, AB, Canada.

Zare, Y., Nejati-Javaremi , A., Rahimi, G. 2007. Detection of polymorphisms in two point of gene associated with Twinning (BMP15) in Shal sheep. The 5 th National Biotechnology Congress of Iran. Tehran, Iran. 483.

Research Journal of Livestock Science

Detection of polymorphism in FecB and BMP15 candidate genes associated with litter size in Moghani flock

References

References

Volume 28, Issue 106 - Serial Number 106
June 2015
Pages 83-90

Detection of polymorphism in FecB and BMP15 candidate genes associated with litter size in Moghani flock

References

References

Volume 28, Issue 106 - Serial Number 106June 2015Pages 83-90

Volume 28, Issue 106 - Serial Number 106
June 2015
Pages 83-90