Effects of α- Linolenic and linoleic fatty acids, with or without trehalose and sucrose on quality of Markhoz goat frozen-thawed spermatozoa

Farshad, Abbas; Farzinpour, Amjad; Mahmoudi, Shahou

doi:10.22092/asj.2017.111309

Document Type : Research Paper

Authors

¹ Faculty staff

² Faculty Staff

³ Ms.S. educuated Student

https://doi.org/10.22092/asj.2017.111309

Abstract

The aim of this study was to evaluate the effects of α- linolenic and linoleic fatty acids, trehalose and sucrose on quality of Markhoz goat frozen-thawed sperm cells. Semen was collected from 4 mature goats using an artificial vagina, evaluated, pooled and divided into 11 aliquots and extended 1:10 with basic tris diluent containing different fatty acids. The motility, progressive motility, viability, acrosome and membrane integrity of frozen spermatozoa have been evaluated after thawing. The results indicated that α- linolenic was significantly the best diluent, while the linoleic acid decreased significantly the quality of spermatozoa, except membrane integrity. Moreover, the use of trehalose and sucrose in diluents decreased significantly the sperm cells characteristics. In addition, the result presented that α- linolenic acid in combination with trehalose improved significantly the rate of acrosome and membrane integrity, but decreased the motility and progressive motility of frozen-thawed spermatozoa. Moreover, the treatments containing all antioxidants and diluents containing linoleic acid and sugars decreased significantly the rate of assessed parameters. In conclusion, the results in this study indicated that using of 20 mg α-linolenic acid in diluent alone and in combination with trehalose can be suitable for cryopreservation of Markhoz goat sperm cells.

Keywords

References

نصیری، ا. ح. 1387. اثر افزودن منبع اسیدهای چرب n-3 و ویتامین E به رقیق کننده بر قابلیت انجماد اسپرم گاو هلشتاین. پایان نامه کارشناسی ارشد علوم دامی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران.

AFRC (Agricultural and Food Research Council). (1998) The nutrition of goat. An advisory manual prepared by the AFRC technical committee on responses to nutrients. CAB International, Wallingford, UK.

Aisen, E.G., Alvarez, L., Venturino, A. and Garde, J.J. (2000). Effect of trehalose and EDTA on cryoprotective action of ram semen diluents. Theriogenology, 53: 1053-1061.

Aisen, E.G., Medina, V.H. and Venturino, A. (2002). Cryopreservation and post-thawed fertility of ram semen frozen in different trehalose concentrations. Theriogenology, 57: 1801-1808.

Aitken, J. and Fisher, H. (1994). Reactive oxygen species generation and human spermatozoa: the balance of benefit and risk. Bioessays, 16: 259–267.

Al-Daraji, H.j., Al-Mashadani, H.A., Al-Hayani, W.K., Al-Hassani, A.S. and Mirza, H.A. (2010). Effect of n-3 and n-6 fatty acid supplemented diets on semen quality in Japanese quail (Coturnixcoturnix japonica). International Journal of Poultry Science, 9: 656-663.

Alvarez, J.G. and storey, B.T. (1983). Taurine, hypotaurine, epinephrine and albumin inhibit lipid peroxidation in rabbit spermatozoa and protect against loss of motility. Biology of Reproduction, 29: 548-555.

Ansari, M., Towhidi, A., Moradi Shahrbabak, M. and Bahreini, M. (2012). Docosahexaenoic acid and alpha-tocopherol improve sperm cryosurvival in goat. Journal of Animal Science, 45: 7-13.

Bilodeau, J.F., Blanchette, S., Gagnon, C. and Sirard, M.A. (2001). Thiols prevent H-O mediated loss of sperm motility in cryopreserved bull semen. Theriogenology, 56: 275-286.

Chakrabarty, J., Banerjee, D., Pal, D., De, J., Ghosh, A. and Majumder, G.C. (2007). Shedding off specific lipid constituents from sperm cell membrane during cryopreservation. Cryobiology, 57: 27-35.

Eiman, M., Aboagla, E., and Terada, T. (2003). Trehalose-enhanced fluidity of the goat sperm membrane and its protection during freezing. Biology of Reproduction, 69: 1245-1250.

Evans, G. and Maxwell, W.M.S. (1987). Salamon’s artificial insemination of sheep and goats. University Press, Sydney, NSW, Australia, 210: 148-149.

Foote, R.H., Chen, Y. and Brockett, C.C. (1993). Fertility of bull spermatozoa frozen in whole milk extender with trehalose, taurine or blood serum. Journal of Dairy Science, 76: 1908-1913.

Graaf, S.P., Peake, K., Maxwell, W.M.C., O'Brien, J.K. and Evans, G. (2007). Influence of supplementing diet with Oleic and Linoleic acid on the freezing ability and sex-sorting parameters of ram semen. Livestock Science, 110: 166–173.

Grady, S.T., Scott, B.D., Brinsko, S.P. and Forrest, D.W. (2009). Dietary supplementation of 2 sources of omega-3 fatty acids and subsequent effects on fresh, cooled, and frozen seminal characteristics of stallions. Reproductive Physiology, Vol 29, No 5.

Khalili, B., Jafaroghli, M., Farshad, A. and Paresh-khiavi, M. (2010). The effects of different concentrations of glycine and systeine on the freezability of Moghani ram spermatozoa. Asian-Aust. Journal of Animal Science, 23: 318-325.

Kozdrowski, P. (2009). The effect of trehalose on post-thaw viability and fertility of European brown hare (Lepus europaeus Pallas, 1778) spermatozoa. Animal Reprodproduction Siencec, 116: 326–334.

Li, G. (2005). Cryopreservation of reproductive cells tissue. Doctoral thesis. University of science and technology of China. Abstract.

Long, J.A. and Kramer, M. (2003). Effect of vitamin E on lipid peroxidation and fertility after artificial insemination with liquid-stored turkey semen. Poultry Science, 82: 1802-1807.

Lopes, S., Jurisicova, A., Sun, J.G. and Casper, R.F. (1998). Reactive oxygen species: potential cause for DNA fragmentation in human spermatozoa. Human Reproduction, 13: 896-900.

Maldjian, A., Pizzi, F., Gliozzi, T. and Cerolini, S. (2005). Changes in sperm quality and lipid composition during cryopreservation of boar semen. Theriogenology, 63: 411–421.

Oscar, G.P., Mosqueda, M.d.L.J. and Carvajal, S.U. (2009). Boar spermatozoa cryopreservation in low glycerol/trehalose enriched freezing media improves cellular integrity. Cryobiology, 58: 287–292.

Parks, J.E. and Graham, J.K. (1992). Effects of cryopreservation procedures on sperm membranes. Theriogenology, 38: 209-223.

Revell, G. and Mrode, R.A. (1994). An osmotic resistance test for bovine semen. Animal Reproduction Science, 36: 77-86.

SAS. 1996. SAS/STAT Software: changes and enhancements through release 6. 12. SAS Institute Inc., Cary, NC, US.

Research Journal of Livestock Science

Effects of α- Linolenic and linoleic fatty acids, with or without trehalose and sucrose on quality of Markhoz goat frozen-thawed spermatozoa

References

References

Volume 30, Issue 114
June 2017
Pages 35-42

Effects of α- Linolenic and linoleic fatty acids, with or without trehalose and sucrose on quality of Markhoz goat frozen-thawed spermatozoa

References

References

Volume 30, Issue 114June 2017Pages 35-42

Volume 30, Issue 114
June 2017
Pages 35-42