The interaction effects of vitamin E and selenium on Arabian ram spermatozoa quality parameters during semen storage in liquid condition

Document Type : Research Paper

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Abstract

The sperm cell membrane is susceptible to irreparable damages due to an increase in reactive oxygen species by process and storage.The aim of this study was to evaluate the effect of vitamin E and selenium antioxidants on the spermatozoa quality of Arabian ram at different times of semen storage under liquid condition.For this purpose, semen collected weakly(Two days a week)from 8 rams in breeding season under the same nutrition and management conditions. All semen samples were mixed immediately and diluted. This experiment was carried out in factorial arrangement with the use of completely randomized design. Treatments were included the different levels of vitamin E(0, 30 and 60 μg/ml),selenium(0, 2 and 4 μg/ml) and semen storage times(0, 2, 4 and 8 hours)Under 4 ° C.The effect of treatments on spermatozoa quality included the progressive motility, viability and morphological defects during various times were assessed. Based on Duncan test, the spermatozoa motility, viability and morphological defect rates were not improved by levels of vitamin E and selenium during semen storage in liquid condition. Evaluation the interaction effect of vitamin E and selenium on spermatozoa quality showed that the vitamin E in 60 μg/ml and both levels of the selenium without vitamin, caused the increase of semen quality than control group(P< 0.001).In treatments there were both vitamin E and selenium, significantly reduced spermatozoa quality compared with the control group(P<0.001).Therefore, the use of vitamin E(60 μg/ml) and selenium(2 μg/ml), antioxidants separately in tris extender and egg yolk improved the semen quality of Arabian ram

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Animal Sciences Journal
Volume 30, Issue 114
June 2017
Pages 43-56

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