Document Type : Research Paper

Authors

• Hossein Moradgholi ¹
• Gholam Reza Dashab ²
• Mehdi Vafa Valleh ³

¹ MS.c of Animal Breeding and Genetics . University of Zabol

² Assistant professor of animal science

³ Assistant Professor, Animal Breeding and Genetics, University of Zabol

Abstract

Sistani beef cattle is a native breed of east of Iran that as a valuable genetic resource in the ‎tropical area could be good a candidate to produce high quantity of muscular‏ ‏carcass portions ‎with high‏ ‏efficiency. In order to explore the Mitochondrial transcription factor A gene ‎‎(TFAM) polymorphisms using PCR-RFLP, 150 Sistani cows were randomly selected and ‎then blood samples were taken from the jugular vein of each animal individually. ‎Thereafter, blood samples were‏ ‏salting-out and electrophoresed with 1% agarose gel. The ‎position of the TFAM promoter region amplified by PCR and PCR products with 801bp ‎length were sliced by‏ ‏BsuRI enzyme. Digestion products on 3% agarose gel were shown by ‎electrophoresis and staining with ethidium bromide. The patterns of digestion in AA ‎homozygotes, CC homozygotes, and AC heterozygotes were as follows: three bands with ‎‎152, 187, and 462bp; four bands with 83, 104, 152, and 462bp; and five bands with 83, 104, ‎‎152, 187, and 462bp, respectively. The results showed that the position of TFAM population ‎deviated from the Hardy-Weinberg equilibrium (P <0.05). Shannon index (I), Nei index, ‎observed heterozygosity, and expected heterozygosity were 0.69, 0.49, 0.37, and 0.50, ‎respectively. In conclusion, no significant differences between genotypes of Sistani cattle ‎were observed for TFAM gene polymorphism and growth traits.‎

Keywords

• Sistani‏ ‏cattle
• ‏Growth traits
• Daily gain
• TFAM gene