Document Type : Research Paper
Authors
Abstract
This experiment was conducted to investigate the effect of transferring bacteria Bacillus licheniformis, Ochrobactrum intermedium and Mycobacterium paludicola, with degrading lignin and lignocellulose potential isolated from termite gut to the rumen liquor, on in vitro gas production parameters and digestibility of nutrients in wheat straw and date leaves as a substrate. Each substrate was incubated with rumen liquor that was inoculated with each of the isolated bacteria using completely randomized design, and compared with control treatment (rumen liquor without inoculation). In both substrates, gas parameters, ruminal dry matter (DM) and organic matter (OM) digestibility, estimated metabolizable energy, partitioning factor, pH and two step digestibility of DM, OM, neutral detergent fiber and acid detergent fiber were similar (P>0.05) between the experimental treatments. Inoculation of isolated bacteria to the rumen liquor in both substrates significantly increased (P<0.05) ammonia nitrogen concentration in compared to control treatment. In both substrates, highest and lowest ammonia nitrogen was observed in treatment inoculated with Ochrobactrum and control treatment, respectively. Results of the present study indicated that inoculation of lignocellulosic degrading bacteria isolated from termite gut to rumen liquor did not improve in vitro gas production parameters and nutrient digestibility of wheat straw and date leaves. However, they increased ammonia nitrogen concentration.
Keywords
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