Effect of combination of 2, 4-dinitrophenol as a targeted antioxidant and luteolin as a non-targeted antioxidant on functional parameter of rooster sperm during chilling storage at 4°C

Nazari, Mahdi; Daghigh Kia, Hossein; Najafi, Abozar

doi:10.22092/asj.2020.342828.2058

Document Type : Research Paper

Authors

¹ Department of Animal Science. Faculty of Agriculture. University of Tabriz. Tabriz, Iran

² University of Tabriz

³ Department of Animal Science

https://doi.org/10.22092/asj.2020.342828.2058

Abstract

In the process of sperm storage, production of reactive oxygen species (ROS) and reduction of antioxidant activity cause sperm damage. The most damage related to high ROS production and inadequate antioxidant penetration into mitochondria attacks this organelle. Purpose: Combination of antioxidants was used to reduce oxidative damage. This study investigated the effect of different levels of combination of targeted antioxidant 2, 4-dinitrophenol and non-targeting luteolin on semen dilution on sperm parameters under in vitro storage at 4°C for 48 h. Semen samples were collected from 15 rooster at 28 weeks of age. After initial evaluations, semen samples were mixed with each other. After diluting the samples and adding different levels of antioxidants, treatment 1 (0.5 nM 4,2-di-nitrophenol + 1 μM luteolin), treatment 2 (0.5 nM 4,2-di-nitrophenol +3 μM luteolin), treatment 3 (0.75 nM) 4,2-Ditrophenol + 1 μM luteolin), 4 (0.75 nM 4,2-Dithinrophenol +3 μM luteolin) from combined antioxidant and storage of samples at 4°C for 48 h in refrigerator quantitative parameters were evaluated at 1, 24, 48 h after storage at 4°C. The parameters evaluated had a significant decrease over time. But the samples treated with the combination antioxidant significantly increased (P <0.05) in the measured parameters independent of the time and the interaction effect of the treatment on time. Also, addition of 3, 4, 5 and 6 treatments significantly decreased malondialdehyde concentration and treatment's 6 significantly decreased unhealthy spermatozoa (P <0.05).

Keywords

20.1001.1.25886436.1400.34.132.1.0

References

دقیق‌کیا، ح. صادقی‌صادق‌آباد، ف. محمدزاده، ح. واثقی‌دودران، ح. و اشرفی، ا. )1395(. اثر عصاره مرزنجوش به عنوان آنتی اکسیدان طبیعی بر کیفیت اسپرم منجمد-یخگشایی شده قوچ. پژوهش‌های علوم دامی. شماره26،1ص ص. 20-111.

صفا، س.، مقدم، غ.، جعفری جوزانی، ر.، دقیق‌کیا، ح.، جانمحمدی، ح. و نعمتی، ذ،ا. )1395(. بررسی اثرات سطوح مختلف ویتامین E و نانوسلنیوم بر فراسنجه‌های کیفی اسپرم خروس نژاد لگهورن طی نگهداری در دمای °C4. پژوهش‌های علوم دامی. شماره26، ص ص.70-59.

محمدی، ن. و دقیقکیا، ح. )1398(. مهار تولید ROS از طریق افزودن آنتی اکسیدان هدفمند 2-4-دی‌نیتروفنول و تأثیر آن بر عملکرد اسپرم منجمد-یخ‌گشایی خروس. علوم دامی. شماره32، ص ص.16-3.

Agarwal, A. and Anandh Prabakaran, S. (2005). Oxidative stress and antioxidants in male infertility: a difficult balance. International Journal of Reproductive BioMedicine. 3 (1): 1-0.

Altawash, A.S.A., Shahneh, A.Z., Moravej, H., Ansari, M. (2017). Chrysin-induced sperm parameters and fatty acid profile changes improve reproductive performance of roosters. Theriogenology. 104: 72-79.

An, F., Wang, S., Yuan, D., Gong, Y. and Wang, S. (2016). Attenuation of oxidative stress of erythrocytes by plant-derived flavonoids, orientin and luteolin. Evidence-Based Complementary and Alternative Medicine. 2016.

Bilodeau, J.F., Chatterjee, S., Sirard, M.A. and Gagnon, C. (2000). Levels of antioxidant defenses are decreased in bovine spermatozoa after a cycle of freezing and thawing. Molecular Reproduction and Development: Incorporating Gamete Research. 55 (3): 282-288.

Brand, M.D. and Esteves, T.C. (2005). Physiological functions of the mitochondrial uncoupling proteins UCP2 and UCP3. Cell metabolism. 2 (2): 85-93.

Cheng, H.Y., Hsieh, M.T., Tsai, F.S., Wu, C.R., Chiu, C.S., Lee, M.M., Xu, H.X., Zhao, Z.Z. and Peng, W.H. (2010). Neuroprotective effect of luteolin on amyloid β protein (25–35)‐induced toxicity in cultured rat cortical neurons. Phytotherapy research. 24 (S1): S102-S108.

Daghigh Kia, H., Jafari, S., Moghaddam, G., Ebrahimi, M. and Najafi, A. (2018). Effects of Different Concentrations of L-Carnitine in Lecithin-Based Semen Extender on Semen Quality of Ghezel Ram after Freeze Thawing Process. Research On Animal Production (Scientific and Research). 9 (19): 48-53.

Dong, Q., Tollner, T.L., Rodenburg, S.E., Hill, D.L. and VandeVoort, C.A. (2010). Antioxidants, Oxyrase, and mitochondrial uncoupler 2, 4-dinitrophenol improved postthaw survival of rhesus monkey sperm from ejaculates with low cryosurvival. Fertility and sterility. 94 (6): 2359-2361.

Fang, L., Bai, C., Chen, Y., Dai, J., Xiang, Y., Ji, X., Huang, C. and Dong, Q. (2014). Inhibition of ROS production through mitochondria-targeted antioxidant and mitochondrial uncoupling increases post-thaw sperm viability in yellow catfish. Cryobiology. 69 (3): 386-393.

Gallon, F., Marchetti, C., Jouy, N. and Marchetti, P. (2006). The functionality of mitochondria differentiates human spermatozoa with high and low fertilizing capability. Fertility and sterility. 86 (5): 1526-1530.

Harper, J., Dickinson, K. and Brand, M. (2001). Mitochondrial uncoupling as a target for drug development for the treatment of obesity. Obesity Reviews. 2 (4): 255-265.

Hernández, M., Roca, J., Gil, M.A., Vázquez, J.M. and Martínez, E.A. (2007). Adjustments on the cryopreservation conditions reduce the incidence of boar ejaculates with poor sperm freezability. Theriogenology. 67 (9): 1436-1445.

Jamshidi, M., Ahmadi Ashtiani, H., Rezazadeh, S., Fathi Azad, F., Mazandarani, M. and Khaki, A. (2010). Study and comparison of phenolic compounds and antioxidant activity of some native plant species of Mazandaran. Quarterly J. Med. Plants. 9 (34): 178-183.

Lin, Y., Shi, R., Wang, X. and Shen, H.-M. (2008). Luteolin, a flavonoid with potential for cancer prevention and therapy. Current cancer drug targets. 8 (7): 634-646.

Liu, R., Meng, F., Zhang, L., Liu, A., Qin, H., Lan, X., Li, L. and Du, G. (2011). Luteolin isolated from the medicinal plant Elsholtzia rugulosa (Labiatae) prevents copper-mediated toxicity in β-amyloid precursor protein Swedish mutation overexpressing SH-SY5Y cells. Molecules. 16 (3): 2084-2096.

Long, J. and Kramer, M. (2003). Effect of vitamin E on lipid peroxidation and fertility after artificial insemination with liquid-stored turkey semen. Poultry science. 82 (11): 1802-1807.

Masoudi, R., Asadzadeh, N.and Sharafi, M. (2020). The mitochondria-targeted antioxidant Mito-TEMPO conserves rooster's cooled semen quality and fertility potential. Theriogenology.156:236-241

Najafi, A., Kia, H.D., Mohammadi, H., Najafi, M.H., Zanganeh, Z., Sharafi, M., Martinez-Pastor, F. and Adeldust, H. (2014). Different concentrations of cysteamine and ergothioneine improve microscopic and oxidative parameters in ram semen frozen with a soybean lecithin extender. Cryobiology. 69 (1): 68-73.

Sapanidou, V.G., Margaritis, I., Siahos, N., Arsenopoulos, K., Dragatidou, E., Taitzoglou, I.A., Zervos, I.A., Theodoridis, A. and Tsantarliotou, M.P. (2014). Antioxidant effect of a polyphenol-rich grape pomace extract on motility, viability and lipid peroxidation of thawed bovine spermatozoa. Journal of biological research-Thessaloniki. 21 (1): 19.

Seifi-Jamadi, A., Zhandi, M. and Ansari, M. (2019). The effect of Chrysin inclusion to Beltsville extender on cooling storage of rooster sperm. Journal of Animal Research. 32 (1): 36-48.

Skulachev, V.P., Anisimov, V.N., Antonenko, Y.N., Bakeeva, L.E., Chernyak, B.V., Erichev, V.P., Filenko, O.F., Kalinina, N.I., Kapelko, V.I. and Kolosova, N.G. (2009). An attempt to prevent senescence: a mitochondrial approach. Biochimica et Biophysica Acta (BBA)-Bioenergetics. 1787 (5): 437-461.

Yahyazadeh, A. and Altunkaynak, B. (2019). Protective effects of luteolin on rat testis following exposure to 900 MHz electromagnetic field. Biotechnic & Histochemistry. 94 (4): 298-307.

Yamada, Y., Akita, H., Kogure, K., Kamiya, H. and Harashima, H. (2007). Mitochondrial drug delivery and mitochondrial disease therapy–an approach to liposome-based delivery targeted to mitochondria. Mitochondrion. 7 (1-2): 63-71.

Yang, J., Kim, J.-S., Jeong, H.J., Kang, H.-H., Cho, J.-C., Yeom, H.-M. and Kim, M.J. (2011). Determination of antioxidant and α-glucosidase inhibitory activities and luteolin contents of Chrysanthemum morifolium Ramat extracts. African Journal of Biotechnology. 10 (82): 19197-19202.

Research Journal of Livestock Science

Effect of combination of 2, 4-dinitrophenol as a targeted antioxidant and luteolin as a non-targeted antioxidant on functional parameter of rooster sperm during chilling storage at 4°C

References

References

Volume 34, Issue 132
December 2021
Pages 3-14

Effect of combination of 2, 4-dinitrophenol as a targeted antioxidant and luteolin as a non-targeted antioxidant on functional parameter of rooster sperm during chilling storage at 4°C

References

References

Volume 34, Issue 132December 2021Pages 3-14

Volume 34, Issue 132
December 2021
Pages 3-14