Document Type : Research Paper

Authors

1 ASRI

2 Tabriz University

3 Member of Scientific Board

4 Researcher of the Institute of Animal Sciences of the country Address: Dehghan Villa 1, Shahid Beheshti St., Iran Institute of Animal Sciences, Karaj.

Abstract

The current study was aimed to assess the influence of adding different concentrations of CoQ10 on the freezability of buck sperm. For this purpose, five adult Saanen bucks were used. After semen collection by using an artificial vagina and primary evaluation, semen samples were diluted with tris extender supplemented with different CoQ10 (0, 0.1, 1, 10 and 100 μM) concentraitons and were frozen. Motility, membrane integrity, morphology, mitochondria activity, acrosome integrity, viability and Malondialdehyde (MDA) concentration were evaluated after thawing. The results showed that using 10 and 100 μM concentrations of CoQ10 had the highest and lowest effect on the preservation of sperm qualitative parameters during the freezing process, respectively. In this way the highest percentages of total motility, progressive motility, membrane integrity, mitochondria activity, acrosome integrity and viability and lowest MDA concentration as a marker of lipid peroxidation were observed in extenders containing 10 μM CoQ10. Therefore, supplementation of freezing medium with CoQ10 could be an efficient method to preserve the quality of buck's semen.

Keywords

Main Subjects

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