Antioxidant effect of aqueous and alcoholic extracts of olive leaves on performance, quality characteristics of eggs, biochemical blood, and immune response of laying hens.

Document Type : Research Paper

Authors

1 University of Birjand

2 Department of Animal Science, Faculty of Agriculture, University of Birjand

3 Associate Professor, Department of Basical Sciences Faculty of Veterinary medicine

Abstract

Olive leaf extract (OLE) can be used as a rich source of antioxidants due to its polyphenol compounds. The purpose of this research was to measure the antioxidant activity and the effect of aqueous (AOLE) and alcoholic (HOLE) extracts of olive leaves on performance, quality characteristics of eggs, biochemical blood serum, hematology, and immune response of laying hens. This experiment was conducted with 440 high-line laying hens (25 weeks old) in the form of 11 experimental treatments, with 5 replications and each replication including 8 hens for three periods of 28 days in a completely randomized design with five levels of AOLE (200, 400, 600, 800 and 1000 mg/kg) and five levels of HOLE (200, 400, 600, 800 and 1000 mg/kg). The DPPH method was used to measure the antioxidant activity of the extract. Functional traits were measured periodically. Blood was taken from the wing vein for serum biochemical and hematological analysis. Injection of the Newcastle vaccine was performed on the 40th day of the experiment, and blood was taken from the wing vein seven days later. The results showed that birds receiving 1000 levels of AOLE and HOLE had the highest percentage of production compared to the control treatment. The highest number of white blood cells and heterophils and antibody titers against Newcastle virus were observed in birds treated with 1000 HOLE levels. In general, the use of 1000 mg/kg of aqueous and alcoholic extract of olive leaves improves the performance and quality of laying eggs.

Keywords

Main Subjects


آگاه م ج، نصیری مقدم ح، گلیان ا، راجی ا، فرهوش ر، زربان ا. (1394). تأثیر عصاره برگ زیتون (Olea europaea L.) و روغن کنجد (Sesamum indicum L.) در جیره بر ریخت شناسی روده کوچک و برخی فراسنجه های خونی جوجه های گوشتی. نشریه دامپزشکی ایران، 11(2): 33-43
آموزمهر ا، دستار ب، شمس شرق زره داران و س. (1390). تاثیر سطوح مختلف سبوس برنج خام و عمل آوری شده در جیره بر عملکرد جوجه های گوشتی. علوم دامی- پژوهش و سازندگی، 87: 27-33.
احسانی م.، و ترکی م. (1390). تاثیر استفاده از تفاله زیتون با و بدون پودر سیر و آویشن در جیره‌های غذایی بر فراسنجه‌های لاشه و عملکرد جوجه‌های گوشتی. مجله علوم دامی ایران. 42 (4)، 311-320.
پوررضا، جواد. (1389). تغذیه مرغ( تالیف اسکات، نسهایم و یانگ ). انتشارات ارکان، اصفهان ص 223.
سروش س ز، حسینی واشان س ج، افضلی ن، اله رسانی ع. (1399). اثر عصاره برگ و روغن زیتون بر عملکرد رشد، ریخت‌شناسی ایلئومو گوارش پذیری مواد مغذی در بلدرچین ژاپنی. پژوهش‌های تولیدات دامی، 11(28): 11-21.
جلال‌فرد ف.، پارسایی س.، هوشمند م.، و نقی‌ها نجف آبادی ر. (1399). اثرات برگ زیتون و پونه بر عملکرد، فراسنجه‌های خونی و جمعیت میکروبی ایلئومی در جوجه‌های گوشتی. میکروبیولوژی دامپزشکی. 16(2)، 125-136.
فرخوی م، خلیقی سیگارودی ت، نیک نفس ف. (1373). راهنمای کامل پرورش طیور. ناشر:شرکت پژوهش توسعه کشاورزی کوثر
Afsari M., Mohebbifar A. and Torki M. (2013). Effects of phytase supplementation of low phosphorous diets include olive pulp and date pits on productive performance of laying hens, egg quality traits and some blood parameters. Annual Review Research in Biology. 3(4):777-793.
Agah, M. J., Mirakzehi, M. T., & Saleh, H. (2019). Effects of olive leaf extract (Olea europea L.) on growth performance, blood metabolites and antioxidant activities in broiler chickens under heat stress.  Journal of Animal & Plant Sciences, 29(3).
Agostini P. S., Sol-Oriol D., Nofraras M., Barroeta A. C., Gasa J. and Manzanilla E. G. (2012). Role of in-feed clove supplementation on growth performance, intestinal microbiology, and morphology in broiler chicken. Livestock Science, 147:113–118.
Ajakaiye JJ, Perez-Bello A, Molloneda-Trujillo A. (2011). Impact of heat stress on egg quality in layer hens supplemented with l-ascorbic acid and dltocopherol acetate. Veterinary Archives.81:119-132.
Al-Harthi M. A. (2016). The efficacy of using olive cake as a by-product in broiler feeding with or without yeast. Italian Journal of animal science. 15:3,512-520.
Amaral J.S., Seabra, R.M., Andrade P.B., Valentao P., Pereira J.A. and Ferreres F. (2004). Phenolic profile in the quality control of walnut (Juglans regia L.) leaves. Food Chemistry, 88: 373-379.
An BK., Kwon HS., Lee BK., Kim JY., You SJ. and Kim JM. (2010). Effects of dietary skullcap (Scutellaria baicalensis) extract on laying performance and lipid oxidation of chicken eggs. Asian Australasian Journal of Animal Science. 23:772-776.
Banerjee A. and Dasgupta N. (2005). In vitro study of antioxidant activity of Syzygium cumini fruit. Food Chemistry. 90: 727-733.
Benavente-Garcia O., Castillo J., Lorente J., Ortuno A. and Del Rio J. A. (2000). Antioxidant activity of phenolics extracted from OleaeuropaeaL. leaves. Food Chemistry. 68:457-462
Bouaziz M. and Sayadi S. (2005). Isolation ana evaluation of antioxidants from leaves off a Tunisian cultivar olive tree. European Jornal of Lipid Science and Technology, 107: 497-504
Bravo D., Pirgozliev V. and Rose S.P. (2014). A mixture of carvacrol, cinnam aldehyde, and capsicum oleoresin improves energy utilization and growth performance of broiler chicken fed maize based diet. Journal of Animal Sciences, 92: 1531-1536.
Cayan H. and Erener G. (2015). Effect of olive leaf (Olea europaea) powder on laying hens performance, egg quality and egg yolk cholesterol levels. Asian Australasation Journal of Animal Sciences, 28: 538-543.
Chuah A.M., Lee Y.C.,Yamaguchi T.,Takamura H.,Yin J. and Matoba T. (2008). Effect of cooking on the antioxidant properties of coloured peppers. Food chemistry, 11(1): 20-28
Ding X., Du J., Zhang K., Bai S., Zeng Q., Peng H., Xuan Y., Su Z. and Wang J. (2020). Tandem mass tag-based quantitative proteomics analysis and gelling properties in egg albumen of laying hens feeding tea polyphenols. Poultry Science, 99: 430–440.
Draper H.H. and M. Hadley. (1990). MDA determination as index of lipid peroxidation. Methods in Enzymology, 186: 421- 430.
Florou-Paneri, Palatos P., Govaris G., Botsoglou A., Giannenas D. and Ambrosiadis I. (2005). Oregano Herb Versus Oregano Essential Oil as Feed Supplements to Increase the Oxidative Stability of Turkey Meat. International Journal of Poultry Science, 4: 866–871.
Grcevic M., Kralik Z., Kralik G., Galovic D., Radisic z. and Hanzek D. (2019). Quality and oxidative stability of eggs laid by hens fed marigold extract supplemented diet. Poultry Science, 98: 333–344
Gurbuz E., Balevi T., Kurtoglu V., Coskun B., Oznurlu Y., Kan Y. and Kartal M. (2010). Effects of Echinacea extract on the performance, antibody titres, and intestinal histology of layer chicks. British Poultry Science, 51: 805-810.
Jahanian R. and Rasouli E. (2011). Effect of dietary inclusion of olive meal on performance and blood lipid metabolites in laying hens. 18th European Symposium on Poultry Nutrition.
Jin, L.,Ho, Y.,Abdullah, N., and Jalaludin, S. (2000). Digestive and bacterial enzyme activities in broilers fed diets supplemented with Lactobacillus cultures. Poultry Science, 79(6): 886-891
Kothari D., Oh J.S., Kim J.H., Lee W.D. and Kim S.K. (2021). Effect of Dietary Supplementation of Fermented Pine Needle Extract on Productive Performance, Egg Quality, and Serum Lipid Parameters in Laying Hens. Animals, 11: 147-165.
Lee O.H., Lee B.Y., Lee J., Lee H.B., Son J.Y., Park C.S., Shetty K. and Kim Y.C. (2009). Assessment of phenolics-enriched extract and fractions of olive leaves and their antioxidant activities. Bioresource Technology, 100: 6107-6113.
Lim KS., You SJ., An BK. and Kang CW. (2006). Effects of dietary garlic powder and copper on cholesterol content and quality characteristics of chicken eggs. Asian Australasian Journal of Animal Science, 19:582-586
Migliorini M.J., Boiago M.M., Stefani L.M., Zampar A., Roza L.F., Barreta M., Arno A., Robazza W.S., Giuriatti J. and Galvao A.C. (2019). Oregano essential oil in the diet of laying hens in winter reduces lipid peroxidation in yolks and increases shelf life in eggs. Journal of thermal biology, 85: 102409.
Olukosi O.A., Xiao W. and Jia J. (2018). Peptide supplementation to nutrient-adequate diets enhanced internal egg quality during storage in hens at peak production. Journal of Science and Food Agriculture, 98: 1850–1855.
Pereira G.C.C., Costa F.G.P., Silva J.H.V.d., Pascoal L.A.F., Lima C.A.B.d., Bittencourt L.C., Sechinato A.d.S. and Hermes R.G. (2018). Different trace mineral sources and recommendations in the performance and quality of eggs from Dekalb White layers. Brazilian journal of animal science, 47: 201-225.
Prasad K. and Kalra J. (1993). Oxygen free radicals and hypercholesterolemic atherosclerosis: effect of vitamin E. American heart journal, 125(4): 958-973.
Santos Ricalde R., Segura Correa J. and Sarmiento Franco L. (2019). Egg quality during storage of eggs from hens fed diets with crude palm oil. Cordoba magazine, 24: 7297–7304
Sarica S. and Toptas S. (2014). Effects of dietary oleuropein supplementation on growth performance, serum lipid concentrations and lipid oxidation of Japanese quails. Journal of Animal Physiology and Animal Nutrition, 98(6):1176-1186
Singh, A.K., Cabral, C., Kumar, R., Ganguly, R., Rana, H.K., Gupta, A.and (2019). Beneficial effects of dietary polyphenols on gut microbiota and strategies to improve delivery efficiency. Nutrients. 11: 2216; doi:10.3390/nu11092216
Sung J.H., Choi S.J., Lee S.W., Park K.H. and Moon T.W. (2004). Isoflavones found in Korean soybean paste as 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors. Bioscience Biotechnology and Biochemistry, 8:1051-1058.
Surai PF. (2000). Effect of selenium and vitamin E content of maternal diet on the antioxidant system of the yolk and the developing chicks. British Poultry Science, 41:235-243.
Vlaicu P.A. and Panaite T.D. (2021). Effect of dietary pumpkin (Cucurbita moschata) seed meal on layer performance and egg quality characteristics. Animal Bioscience, 35: 236–246.
Wenk C. (2002). Herbs, botanicals and other related substances. WPSA-Bremen. Germany
Wintergerst ES., Maggini S. and Hornig DH. (2007). Contribution of selected vitamins and trace elements to immune function. Nutrition Metabolism, 51:301-23.
Yue HY., Wang J., Qi XL., Ji F., Liu MF. and Wu SG. (2011). Effects of dietary oxidized oil on laying performance, lipid metabolism, and apolipoprotein gene expression in laying hens. Poultry Science, 90:1728-1736.
Zangeneh S. and Torki M. (2011). Effects of b-mannanase supplementing of olive pulp included diet on performance of laying hens, egg quality characteristics, humoral and cellular immune response and blood parameters. Global Veterinary, 7: 391-398.
Zhang J., Zhang M., Liang W., Geng Z. and Chen X. (2020). Green tea powder supplementation increased viscosity and decreased lysozyme activity of egg white during storage of eggs frjmom Huainan partridge chicken. Italian Journal of Animal Science, 19: 586–592
Zhu Y.F., Wang J P., Ding X M., Bai S P., Qi S.R.N., Zeng Q.F., Xuan Y., Su Z.W. and Zhang K.Y. (2020). Effect of different tea polyphenol products on egg production performance, egg quality and antioxidative status of laying hens. Animal Feed Science and Technology. 26: 114-125