Document Type : Research Paper

Authors

1 Razi University

2 ASRI

3 Assistant professor of Animal Science Research Institute of Iran

Abstract

The aim of this study was to evaluate the sperm quality preservation in sheep using dietary Zinc nanoparticles during chilling process. In this experiment, 15 adult rams were divided into 3 equal groups and received 0, 35 and 70 ppm Zinc nanoparticles, respectively. Then, semen samples were collected and after dilution in cooling extender were stored at 4 ˚C until 48 hours. Sperm quality was evaluated at 0, 24 and 48 hours after chilling and quality parameters such as total motility, progressive motility, viability, membrane integrity, mitochondrial activity and lipid peroxidation were assessed. In results, during evaluation times (0, 24 and 48 hours) of cooling periods, using treatment 70 increased total motility, progressive motility, viability, membrane integrity, mitochondrial activity and decreased lipid peroxidation in ram chilled sperm. In conclusion, it was observed that using Zinc nanoparticles in animal ration could be a suitable method to conserve ram sperm quality during chilling process.

Keywords

Amem, M. H., & Al-Daraji, H. J. (2011). Effect of dietary zinc on semen quality of Cobb 500 broiler breeder males. International Journal of poultry science10(6), 477-482.
Baumber, J., Ball, B. A., GRAVANCE, C. G., Medina, V., & Davies‐Morel, M. C. (2000). The effect of reactive oxygen species on equine sperm motility, viability, acrosomal integrity, mitochondrial membrane potential, and membrane lipid peroxidation. Journal of andrology21(6), 895-902.
Bréque, C., Surai, P., & Brillard, J. P. (2003). Roles of antioxidants on prolonged storage of avian spermatozoa in vivo and in vitro. Molecular Reproduction and Development: Incorporating Gamete Research66(3), 314-323.
Dani, V., & Dhawan, D. K. (2005). Radioprotective role of zinc following single dose radioiodine exposure to red blood cells of rats. Indian Journal of Medical Research122(4), 338.
Dawei, A. I., Zhisheng, W., & Anguo, Z. (2010). Protective effects of Nano-ZnO on the primary culture mice intestinal epithelial cells in in vitro against oxidative injury. World Journal of Agricultural Sciences6(2), 149-153.
Hidiroglou, M., & Knipfel, J. E. (1984). Zinc in mammalian sperm: a review. Journal of dairy science67(6), 1147-1156.
Khodaei-Motlagh, M., Masoudi, R., Karimi-Sabet, M. J., & Hatefi, A. (2022). Supplementation of sperm cooling medium with Zinc and Zinc oxide nanoparticles preserves rooster sperm quality and fertility potential. Theriogenology183, 36-40.
Klotz, L. O., Kröncke, K. D., Buchczyk, D. P., & Sies, H. (2003). Role of copper, zinc, selenium and tellurium in the cellular defense against oxidative and nitrosative stress. The Journal of nutrition133(5), 1448S-1451S.
Kotdawala, A. P., Kumar, S., Salian, S. R., Thankachan, P., Govindraj, K., Kumar, P., ... & Adiga, S. K. (2012). Addition of zinc to human ejaculate prior to cryopreservation prevents freeze-thaw-induced DNA damage and preserves sperm function. Journal of Assisted Reproduction and Genetics29, 1447-1453.
Lewis-Jones, D. I., Aird, I. A., Biljan, M. M., & Kingsland, C. R. (1996). Andrology: Effects of sperm activity on zinc and fructose concentrations in seminal plasma. Human reproduction, 11(11), 2465-2467. doi: 10.1093/oxfordjournals.humrep.a019138.
Mankad, M., Sathawara, N. G., Doshi, H., Saiyed, H. N., & Kumar, S. (2006). Seminal plasma zinc concentration and α-glucosidase activity with respect to semen quality. Biological trace element research110, 97-106.
Masoudi, R., Esmaeilkhanian, S., Sharafi, M., Abdollahi, Z., Jafari, V., Hatefi, A., ... & Banabazi, M. H. (2022). Cysteamine enhances quality and fertility potential of rooster semen in cooled storage. Theriogenology177, 29-33.
Mennella, M. R. F., & Jones, R. (1980). Properties of spermatozoal superoxide dismutase and lack of involvement of superoxides in metal-ion-catalysed lipid-peroxidation and reactions in semen. Biochemical Journal191(2), 289-297.
Prasad, A. S. (1993). Essentiality and toxicity of zinc. Scandinavian Journal of Work, Environment & Health, 134-136.
Underwood, E. J., & Somers, M. (1969). Studies of zinc nutrition in sheep. I. The relation of zinc to growth, testicular development, and spermatogenesis in young rams. Australian Journal of Agricultural Research20(5), 889-897.
Yang, H., Roberts, L. J., Shi, M. J., Zhou, L. C., Ballard, B. R., Richardson, A., & Guo, Z. M. (2004). Retardation of atherosclerosis by overexpression of catalase or both Cu/Zn-superoxide dismutase and catalase in mice lacking apolipoprotein E. Circulation research95(11), 1075-1081.
Zvekić, D., Srdić, V. V., Karaman, M. A., & Matavulj, M. N. (2011). Antimicrobial properties of ZnO nanoparticles incorporated in polyurethane varnish. Processing and Application of Ceramics5(1), 41-45.