Golafshan Mahaki; Manouchehr Souri; Reza Masoudi; nader asadzadeh
Abstract
The aim of this study was to investigate the effect of dietary zinc nanoparticles (ZnNPs) on the quantitative and qualitative characteristics of ram semen. For this purpose, 15 adult Zandi rams (3 to 4 years old) were used. Rams were fed the following diets during 60 days: 1) the base diet, 2) base diet ...
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The aim of this study was to investigate the effect of dietary zinc nanoparticles (ZnNPs) on the quantitative and qualitative characteristics of ram semen. For this purpose, 15 adult Zandi rams (3 to 4 years old) were used. Rams were fed the following diets during 60 days: 1) the base diet, 2) base diet containing 35 mg/kg zinc nanoparticles and 3) base diet containing 70 mg/kg zinc nanoparticles. Sperm collection was done from the first day of the experiment for 60 days and once every 10 days by artificial vagina. Quantitative and qualitative sperm parameters including semen volume, number of spermatozoa, motility, membrane integrity and viability were evaluated. The results showed that diet supplementation with 70 mg/kg of zinc nanoparticles improved the number of sperm, motility, progressive motility, membrane integrity and viability compared to the control group and the group receiving 35 mg/kg of zinc nanoparticles (P <0.05). Therefore, adding zinc nanoparticles to the diet is a suitable method to improve semen quality and thus increase reproductive performance in ram.
Mahdi Nazari; Hossein Daghigh Kia; Marzieh Ebrahimi; Abozar Najafi; Mahdieh Mehdipour
Abstract
Mitochondria as sperm energy supplier motors have unique properties including antioxidant non-permeability, so the ROS concentration in this organelle is 5 to 10 times more than that of the plasma membrane. Therefore, the researchers have designed an antioxidant which can cross the mitochondrial membrane ...
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Mitochondria as sperm energy supplier motors have unique properties including antioxidant non-permeability, so the ROS concentration in this organelle is 5 to 10 times more than that of the plasma membrane. Therefore, the researchers have designed an antioxidant which can cross the mitochondrial membrane and is able to inhibit ROS in this organelle to minimize the oxidative damage. Targeted antioxidant 4, 2-dinitrophenol has the ability to cross the mitochondrial membrane. The purpose of this study was to add different levels of targeted antioxidant to semen to purify mitochondria from reactive oxygen species to reduce oxidative damage and improve sperm motility and viability. In this study, 8 Ghezel rams (2 years old) were used for semen collection by artificial vagina in early spring. After initial evaluation, the samples with normal properties were pooled and after dilution process, 0, 0.25, 0.5, 0.75, and 1 nM 4, 2-dinitrophenol were added to the samples and frozen after 2 hours of cooling. After one month, samples were thawed and evaluated for motility parameters, viability, membrane health, sperm abnormality and lipid peroxidation. Results showed that addition of 0.5 nM of the antioxidant significantly increased motility parameters and decreased abnormal sperm (P <0.05). Also suplimentation of 0.5, 0.75 nM 4, 2-dinitrophenol significantly increased viability and membrane integrity and reduced the amount of lipid peroxidation (P <0.05).
Khalil Mirzadeh; Saba Beiranvand; Saleh TabatabaeiVakili; Tahereh mohammadabadi; Amin Kazemizadeh
Abstract
The use of the plant Tribulus Terrestris can improve semen quality in some mammals and birds. However, little information is available about adult male lambs. The purpose of this experiment was to investigate the effect of different levels of Tribulus terrestris on quantitative and qualitative characteristics ...
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The use of the plant Tribulus Terrestris can improve semen quality in some mammals and birds. However, little information is available about adult male lambs. The purpose of this experiment was to investigate the effect of different levels of Tribulus terrestris on quantitative and qualitative characteristics of sperm of mature male lambs. In this study, 18 lambs of the arabi lambes age 3 monthes and average body weight 16.14±2 were divided into three groups of six in a completely randomized design, and received different levels of Tribulus terrestris including three groups: 1) without Tribulus terrestris (control), 2) 15 g/kg DM Tribulus terrestris and 3) 30 g/kg DM Tribulus terrestris for 5 months. The spermatogenesis was carried out by the electrocautery device two times a week from lambs after feeding Tribulus Terrestris. Quantitative and qualitative semen parameters including seminal volume and pH, sperm concentration, motility, survival and morphological abnormalities of sperms were evaluated. Results showed, the lowest semen volume was in the control group (p < 0.05). The highest concentration, motility and survival of sperm were from 15 g of Tribulus terrestris (p < 0.05). The level of 30 g of Tribulus terrestris showed the highest morphological abnormalities of sperm (p < 0.05). Mean pH was not affected by experimental treatments (p < 0.05). In conclusion, considering to all the measured parameters, adding 15 g of Tribulus terrestris per kilogram dry matter to the diet improved the quantitative and qualitative characteristics of sperm in the freshly mature arabi lambs.
Hossein Daghigh Kia; Sepehr Jafari
Abstract
The purpose of this study was to compare the effect of different levels of zinc sulfate on improving the quality of ram semen during freeze-thawing process outside of the reproductive season. Semen samples were collected from five Ghezel ram twice a week using the artificial vagina. Sperm samples were ...
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The purpose of this study was to compare the effect of different levels of zinc sulfate on improving the quality of ram semen during freeze-thawing process outside of the reproductive season. Semen samples were collected from five Ghezel ram twice a week using the artificial vagina. Sperm samples were assigned to four treatment groups including: control, 17.5, 35 and 52.5 μm zinc sulfate, and then the freezing process was performed. After thawing motility, morphology and malondialdehyde levels of sperm were studied. Total and progressive motility of sperm containing the 17.5 µmol/ml zinc sulfate were significantly higher than control group (P <0.05). There was a significant difference in sperm morphology in the group receiving 52.5 μmol of zinc sulfate compared to the control group, which caused 52.5% increase in abnormal sperm (P <0.05). Adding 17.5 μmol of zinc sulfate increased unsignificantly the viability of sperms compared to the control group, but adding 35 and 52.5 μmol decreased significantly (P <0.05). The level of malondialdehyde production in the 35 µmol was lower than the other treatment groups and the control group and there was a significant difference between the groups 17.5 and 35 in comparison with the control group (P <0.05). Therefore, it can be concluded that the use of 17.5 and 35 µmol Zinc sulfate in Ghezel ram diluent semen improves some of the parameters of the sperm after the freeze-thawing process.
seyed kamal hashemian; M. Mehri
Abstract
The objective of the present study was to investigate the preservative effect of green tea extract as a natural antioxidant on sperm quality parameters of rooster in the duration of an entire spermatogenesis cycle. Sixteen rooster divided into four aliquots and consumed diets supplemented with 0, 30, ...
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The objective of the present study was to investigate the preservative effect of green tea extract as a natural antioxidant on sperm quality parameters of rooster in the duration of an entire spermatogenesis cycle. Sixteen rooster divided into four aliquots and consumed diets supplemented with 0, 30, 60, 90 mg of green tea extract/kg of feed. On 0, 14, 28, 42, 56, 70 days after start semen fluid were collected and CASA for sperm motility, progressive motility, linearity (LIN), curvilinear velocity (VCL), amplitude of lateral head displacement (ALH), average path velocity (VAP), straight line velocity (VSL), nonapoptotic sperm and DNA fragmentation (by staining sperm with acridine orange). Treatments 3 and 4 reduced apoptotic sperm and improved some of sperm motion parameters such as ALH (P<0.05). However, in last days of experiment, treatment 4 had adverse effects on VAP and VSL (P<0.05). The least amount of green tea extract (30 mg/kg of feed) improved VAP (P<0.05). The results showed that daily consumption of 60 mg green tea extract/kg of feed, as a natural antioxidant, could possibly lead to improve some of sperm motion parameters and reduced apoptosis.
hamid reza naijian; H. Sadeghi Panah; R. Masoudi
Abstract
This study was designed to investigate the effects of various concentrations of egg yolk (5, 10, 15 and 20%) and Lecithin (1, 1.5 and 2%) in the extenders based on tris (with 5% glycerol) on the Marghoz goat spermatozoa after freezing-thawing. Semen samples were collected by an artificial vagina, twice ...
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This study was designed to investigate the effects of various concentrations of egg yolk (5, 10, 15 and 20%) and Lecithin (1, 1.5 and 2%) in the extenders based on tris (with 5% glycerol) on the Marghoz goat spermatozoa after freezing-thawing. Semen samples were collected by an artificial vagina, twice a week from four matured goat.The extender containing semen was frozen in liquid nitrogen and then was stored until using for assessment. Semen was thawed at 37◦C and then motility and progressive motility were assessed by CASA. Membrane integrity and viability,morphology were assessed as well and Malondialdehiyde (MDA) were were assessed as well. The results of this experiment showed that extender containing 15% egg yolk and 1.5% Lecithin significantly improved motility, progressivemotility and viability compare to other levels (P<0.05). Membrane integrity, morphology, malondialdehiyde and acrosome status were not affected significantly by the different level of egg yolk and Lecithin (P > 0.05). It can be concluded that use of 15% egg yolk in animal extender and 1.5% Lecithin in vegetable extender is suitable for cryopreservation of Marghoz goat semen.
Saleh Tabatabaei Vakili; R. Zeidi; M. Mamoei; Kh. Mirzadeh
Abstract
Glutamine is an amino acid which has antioxidant role. The aim of this study was to investigate the effect of different levels of glutamine into diluent on semen characteristics of Arabi ram in liquid condition. Semen samples were collected weekly from 8 Arabi rams for 8 weeks and pooled. After dilution, ...
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Glutamine is an amino acid which has antioxidant role. The aim of this study was to investigate the effect of different levels of glutamine into diluent on semen characteristics of Arabi ram in liquid condition. Semen samples were collected weekly from 8 Arabi rams for 8 weeks and pooled. After dilution, the pooled semen was divided into 5 parts and received the levels of glutamine (0 or control, 25, 50, 75 and 100 mM). In liquid storage condition under 5°C and different times (1, 24, 48, 72 and 96 hours), semen quality characteristics were evaluated. The glutamine levels have no effects on semen quality in first hour of maintenance (P>0.05). The sperm motility was not affected by treatments during 24 and 48 hours; But, sperm viability in control was lower than 25 mM glutamine (P<0.05). After 72 hours, the lowest sperm motility and viability (except 100 mM of glutamine) was observed in control. While, after 96 hours, only 25 mM and 50 mM dose of glutamine improved the spermatozoa motility and viability (P<0.05). After 24 hours, all glutamine levels were reduced the morphological defect rates of spermatozoa. But, over time, lower levels of glutamine (25 and 50 mM) reduced the morphological defect rates and improved the plasma membrane integrity of spermatozoa (P<0.05). In all times, pH of semen was not affected by treatments (P>0.05). Overall, the use of glutamine especially dose of 25 and 50 mM significantly increased the sperm quality parameters of Arabi ram without effect on semen pH.
