setareh Abedi; Seyyed Javad Hosseini-vashan; Seyed Homayoun Farhangfar; seyyed ehsan ghiasi
Abstract
This study was done to investigate the effect of pomegranate peel extract on growth performance, blood biochemical indices, intestinal morphology and meat quality of broiler fed diet-contained soybean oil. 120 male broilers were used in the form of a completely randomized design with 3 treatments, 4 ...
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This study was done to investigate the effect of pomegranate peel extract on growth performance, blood biochemical indices, intestinal morphology and meat quality of broiler fed diet-contained soybean oil. 120 male broilers were used in the form of a completely randomized design with 3 treatments, 4 repetitions and 10 chicks. Experimental diets were three levels of zero, 300 and 600 mg/kg of pomegranate peel extract (PPE). Pomegranate peel extract had no effect on performance traits including feed intake, body weight and feed conversion ratio at starter and finisher phases, but in the grower period, the levels of 300 and 600 PPE reduced feed intake (from 928 to 777 g) and feed conversion ratio (from 1.77 to 1.31; P <0.05) compare to control. The breast percentage increased from 19.78 in control to 21.95 at the level of 600 PPE and the percentage of abdominal fat decreased from 1.93 in control to 1.27 at the level of 600 PPE (P <0.05). The PPE at levels of 600 reduced concentration of cholesterol (183.68 to 137.15 mg/dl) and LDL (95.63 to 32.93 mg/dl) and the enzyme activity of aspartate dehydrogenase (263.8 to 204.28) compare to control. The villus height (from 1026.88 to 1155.12) and the villus height to crypt depth ratio (5.04 to 5.6) were higher at the 600 PPE compared to the control. The concentration of Malondialdehyde in meat decreased at 15 and 30 days after slaughter with an increase in PPE compared to control.
Mahdi KazemiShorghin; Seyyed Javad Hosseini-vashan; M. Bashtani; Mohsen Mojtahedi
Abstract
This study was to investigate the effect of Portulaca oleracea L. extract(POE) on performance, carcass characteristics, immune response, blood indices and intestinal morphology in broilers under heat stress. 200 Ross 308 single-day-old chicks were used in a completely randomized design with 5 treatments, ...
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This study was to investigate the effect of Portulaca oleracea L. extract(POE) on performance, carcass characteristics, immune response, blood indices and intestinal morphology in broilers under heat stress. 200 Ross 308 single-day-old chicks were used in a completely randomized design with 5 treatments, 4 replications and 10 chicks. Experimental groups were: negative control (no heat stress), four groups under heat stress (positive control), 250, 500 and 750mg of POE per kg diet. Results showed that using POE increased feed intake and body weight compared to the control (P <0.05). Chicks fed POE had lower feed conversion ratio than positive control group (P<0.05). Addition of POE to broiler diet had no effect on relative weight of carcass components including breast, thigh, spleen, bile, pancreas and abdominal fat. The POE reduced the relative weight of heart and liver compared to the positive control and the relative weight of bursa was higher in the chicks fed the POE (P<0.05). The POE increased total antibody titer against sheep red blood cells. Using the POE in broiler diet had no effect on plasma concentration albumin and protein, but the lowest concentration of cholesterol, triglyceride, LDL and highest HDL were observed broilers fed 750 POE. The activity of alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase enzymes decreased during heat stress compared to negative control. POE increased villus height and absorption area compared to control(P<0.05). It is concluded that using POE reduced plasma concentration of cholesterol, LDL, and plasma MDA in broilers under heat stress.
Seyyedeh Zahra Soroush; Seyyed Javad Hosseini-vashan; nazar Afzali; Ali Allahressani
Abstract
To determine the effects of olive pulp(OP) on growth performance, nutrient digestibility, blood indices and intestinal morphology in Japanese quail, A total of 352 days-old quail were allocated to four treatments with four replicates (22 birds each). Excreta were quantitatively collected during 18-21 ...
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To determine the effects of olive pulp(OP) on growth performance, nutrient digestibility, blood indices and intestinal morphology in Japanese quail, A total of 352 days-old quail were allocated to four treatments with four replicates (22 birds each). Excreta were quantitatively collected during 18-21 days of age for measurement digestibility of nutrient. The dietary treatments were included control (0%) and the levels of four, eight and 12 percent OP. At 35 days old, from each replicate, two quails were randomly selected and slaughtered. The dietary treatments did not influence weight gain, feed intake, and FCR. The OP did not influence the relative weight of carcass, breast, thigh, heart, and spleen, but the highest relative weight of liver and gizzard were observed in 12 percent OP (p < 0.05). The ileum villus height was higher in birds fed OP as compared to control(p < 0.05). Crude protein and crude fat digestibility in chickens fed 12% OP and was increased compared to control (p < 0.05). Ash and phosphorous digestibility increased in chickens received 8% OP compare to control (p < 0.05). The olive pulp decreased the plasma MDA concentration as compared to control (p < 0.05), however, treatments had no significant effects on other blood indices. The lowest enzyme activity of AST was observed in the control and the highest enzyme activity of GPx and SOD were observed in 12% OP (p < 0.05). The results were indicated that the inclusion of olive pulp up to 12% had no detrimental effects on growth performance and blood lipid profile but significantly decreased plasma malondialdehyde (p < 0.05).
Amir Javadifar; Seyyed Javad Hosseini-vashan; Mohammad Bagher Montazer Torbati; Yasaman Shamshirgran
Abstract
In order to investigate the effects of different levels of iron nano-oxide(INO) on performance, egg quality parameters and the antioxidant status of laying hens, 96 Bovens layer hens at the production peak(28 weeks) were used. The experiment done for 8 weeks in a completely randomized design with 3 treatments, ...
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In order to investigate the effects of different levels of iron nano-oxide(INO) on performance, egg quality parameters and the antioxidant status of laying hens, 96 Bovens layer hens at the production peak(28 weeks) were used. The experiment done for 8 weeks in a completely randomized design with 3 treatments, 4 replicates and 8 birds in each replicate. Hens were fed with experimental diets containing 0, 50 and 100 mg iron nanooxide/kg of diet. The performance indices included egg production, egg weight, egg mass, feed intake and feed conversion ratio were daily recorded. The qualitative parameters included yolk color index, egg shape, yolk height index, Haugh unit, Eggshell thickness, eggshell strength, Specific gravity and relative weight of albumen, yolk and eggshell were determined at the end of each period(28 days). At the end of experiment, two birds per replicate were bleed and the blood biochemical parameters were studied.The results were revealed that the iron nano-oxide had no effect on performance indices. Total protein and enzyme activity of alanine aminotransferase were not affected by dietary INO.Dietary supplementation of INO decreased the serum concentration of cholesterol, triglyceride, and LDL, and increased serum concentration of HDL, iron, zinc. The antibody titer against Newcastle disease were increased with increasing the levels of dietary INO. The yolk and serum concentration of MDA was increased by the addition of iron nanoparticles. Therefore, the addition of iron oxide supplementation can improve performance indices, some qualitative characteristics of egg and immune response and decrease serum lipid in laying hens.
T. Ghaznavi; Seyyed Javad Hosseini-vashan; N. Afzali; S. E. Ghiasi
Abstract
The aim of this study was conducted to evaluate the effects of processed pomegranate pulp with Hydroxide Calcium on performance egg quality and cholesterol, blood biochemical parameters and antioxidant status of laying hens in peak production of laying hens. A total 200 Hy-Line W-36 at peak production ...
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The aim of this study was conducted to evaluate the effects of processed pomegranate pulp with Hydroxide Calcium on performance egg quality and cholesterol, blood biochemical parameters and antioxidant status of laying hens in peak production of laying hens. A total 200 Hy-Line W-36 at peak production with 32 weeks. The dietary treatments were included the levels of 0, 4, 7 and 10 % of processed pomegranate pulp with Hydroxide Calcium. The performance data including egg percentage, feed intake, egg mass, and FCR were weekly recorded. The results were revealed that processed pomegranate pulp with Hydroxide Calcium(PPP) improved the egg percentage, feed intake, egg mass, and FCR. The egg quality parameters did not affected by dietary treatments with exception that Haugh unit increased in birds received the processed pomegranate pulp. The eggshell parameters involved eggshell weight and specific gravity had no affected by dietary treatments. The eggshell thickness and eggshell resistance was increased when hens fed diets contained PPP. The PPP diets decreased the egg cholesterol and blood cholesterol. The concentration of malondialdehyde decreased with enhancement of the levels PPP in diets. It is concluded that addition of pomegranate pulp with Hydroxide Calcium may be improved the performance, egg quality, and antioxidant status and cholesterol of laying hens.
S. Saberi; H. Sarir; Seyyed Javad Hosseini-vashan
Abstract
To evaluate the effects of different of barberry pulp (BP) on production traits, blood parameters and immune system of broilers under heat stress, an experiment with 160 male Ross 308 chicks in a completely randomized design with 4 treatments, 4 replicates and 10 chicks each were done. A basal corn-soybean ...
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To evaluate the effects of different of barberry pulp (BP) on production traits, blood parameters and immune system of broilers under heat stress, an experiment with 160 male Ross 308 chicks in a completely randomized design with 4 treatments, 4 replicates and 10 chicks each were done. A basal corn-soybean diet (Control) and three other treatments were diets contained the levels of 2.5, 5 and 7.5 percent barberry pulp treatment was fed. The experimental period was 42 days. The cyclic daily heat stress was done from 28 to 42 days. In this period, daily room temperature was increased from 21oC to 38±2°C during 10:00 until 17:00 hours. The treatments had no significant effects on body weight, feed conversion ratio, feed intake, carcass yield, heart, bursa of fabricius and liver. The relative weight of breasts and bursa of fabricus were increased when birds received 7.5 percent Barberry pulps (BP) compared to control. Serum concentration of cholesterol and HDL and liver enzymes (ALT and AST) and immune system were not affected by the levels of BP treatments. However the BP diets decreased the serum concentration of serum triglycerides and LDL. The blood concentration of glucose were increased by different levels of BP. The levels of 2.5% of BP increased the total protein as compared to control. Therefore, this findings were proposed since Barberry pulps had no any negative effects on production parameters and an improvement in the blood and immune system up to 7.5 %, it could be used in HS broilers.
B. Heidari Sadegh; Seyyed Javad Hosseini-vashan; N. Afzali; m. Mojtahedi
Abstract
The purpose of this study was to investigate the effect of acidophilus bacterial isolates from intestine of Coracias Garrulus on performance and carcass characteristics parameters of Japanese quail. A total of 385 chicks were arranged into 35 experimental units with 7 treatments in a completely randomized ...
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The purpose of this study was to investigate the effect of acidophilus bacterial isolates from intestine of Coracias Garrulus on performance and carcass characteristics parameters of Japanese quail. A total of 385 chicks were arranged into 35 experimental units with 7 treatments in a completely randomized design. The treatments were included control, spraying, drinking and sprying+ drinking of the acidophilus bacterial isolates, and spraying, drinking and sprying+ drinking of commercial probiotic. Two birds from each replicate were sacrificed and bleeding, then blood was gathered and plasma was extracted.The results were revealed that addition of acidophilus bacterial isolates and commercial probiotic did not affect feed intake at 47 d. The body weight was increased when birds received acidophilus bacterial isolates and commercial probiotic. The FCR were lower in birds received drinking probiotic and in spraying+ drinking acidophilus bacterial isolates treatments as compared to control. The carcass parameters did not affected by treatments. The results were revealed that addition of acidophilus bacterial isolates and commercial probiotic were decreased the serum cholesterol and LDL concentration of quail. The HDL and triglyceride were not affected by treatments. The protein concentration were increased in chicks received the acidophilus bacterial isolates and commercial probiotic. The antibody response against SRBC and IgM were increased in birds received acidophilus bacterial isolates or commercial probiotic. The jejuna histomorphmetry were showed that height of villi, crypt depth, and the ratios of height of villi to crypt depth were significantly changed.
H. Dashtban; N. Afzali; Seyyed Javad Hosseini-vashan; H. Sarir
Abstract
The aim of this study was to investigate the effect of fruit powder Prosopis farcta on performance, carcass traits, blood biochemical parameters and antioxidant and immune system of broilers under heat stress. The number of 250 male Ross 308 one-day chicks in a completely randomized design with 5 treatments, ...
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The aim of this study was to investigate the effect of fruit powder Prosopis farcta on performance, carcass traits, blood biochemical parameters and antioxidant and immune system of broilers under heat stress. The number of 250 male Ross 308 one-day chicks in a completely randomized design with 5 treatments, 5 replicates per and 10 chicks each was used. The treatments were negative control (non-stress), positive control (stress) and three levels of 1, 3and 5 percent fruit powder Prosopis farcta. The heat stress schedule were done for last two weeks of experiment in a room temperature that was increased to 38 ±2°C during the for 6 hours per day. The results showed that the addition of dried fruit Prosopis farcta in broilers under stress significantly reduced body weight, food consumption, energy efficiency, and protein production index and feed conversion ratio was also significantly increased. The treatments had a significant effect on carcass yield, pancreas, gall bladder and liver. However, the relative weight of the breast, thigh and the relative length of the intestine markedly affected by treatments and increased compared with the control. Cholesterol, glucose, LDL, AST, ALT, LDH and MDA levels were significantly decreased with the addition of dried fruit Prosopis farcta under heat stress condition but the concentration of HDL, total protein and albumin did not significantly change. The antibody titer against SRBC was increased with increasing the levels of Prosopis farcta.
M. Barani; N. Afzali; Seyyed Javad Hosseini-vashan
Abstract
This experiment was conducted to evaluate effect of hempseed on performance, immune response and plasma antioxidative activity and lipid profile in broiler chicks. A total of 250 one-day male broiler chicks (Ross 308 strain) were randomly divided into five treatments with five replicates (10 birds per ...
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This experiment was conducted to evaluate effect of hempseed on performance, immune response and plasma antioxidative activity and lipid profile in broiler chicks. A total of 250 one-day male broiler chicks (Ross 308 strain) were randomly divided into five treatments with five replicates (10 birds per cage). The treatments were: 1) Control diet (H0, no hempseed); 2) the diet contain 5% hempseed (H5); the diet contain 10% hempseed (H10); the diet contain 15% hempseed (H15) and the diet contain 20% hempseed (H20). Data were analyzed using of the PROC GLM of SAS 9.1 software and means were compared using of tukey test. The results of this study indicated that inclusion of hempseed up to 10% into diet significantly (P<0.05) decreased feed intake, body weight but had no significant effect on feed conversion ratio. Also, use of hempseed up to 5% into diet significantly (P<0.05) decreased plasma Malondialdehyde (MDA) content (P<0.05), but had no significant effect on humoral immune response. Plasma total cholesterol, triglyceride and HDL concentrations were not significantly altered. But plasma LDL concentration significantly (P<0.05) decreased in hempseed supplemented groups in compared with control group. In conclusion, although use of hempseed up to 5% into diet improved plasma antioxidative activity, but due to decrease performance, use of it up to 10% into diet was not recommended.
kamal Afzali; Seyyed Javad Hosseini-vashan; nazar Afzali
Abstract
To evaluate the effects of Elaeagnus Angustifolia (EA) and enzyme on serum biochemistry parameters of broiler chicken, 288 one-day-old male Ross 308 broilers were randomly allocated to 32 cages for 8 dietary treatments with 4 replicates. This experiment were done in a completely randomized design with ...
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To evaluate the effects of Elaeagnus Angustifolia (EA) and enzyme on serum biochemistry parameters of broiler chicken, 288 one-day-old male Ross 308 broilers were randomly allocated to 32 cages for 8 dietary treatments with 4 replicates. This experiment were done in a completely randomized design with 4*2 factorial trial involved 4 levels of EA (0, 0.75, 1.5 and 3% EA) and 2 levels of Enzyme (0, 0.05% of Natozyme plus). The data were shown that EA were improved the body weight and feed conversion ratio. Dietary treatment did not affect the triglyceride, and HDL of broiler fed with EA and Enzyme at 28d; but the concentration of LDL and cholesterol was significantly affected, so that the LDL and cholesterol concentration were higher when birds fed 3% EA as compared to birds fed control(P<0/05). The activity of AST and ALT liver enzymes were gradually decreased when birds fed up to 3% of EA. It is concluded that supplementation of EA up to 3% may improve performance and decrease cholesterol and LDL concentration of broiler.
A. Saboor Gholezoo1; N. Afzali; S.M. Hoseini; S.J. Hoseini Vashan
Abstract
To study the effects of different levels of surplus Jujube fruit (SJF) on growth performance parameters, carcass yield and some biochemical parameters, 320 Ross 308 broiler chicks were randomly divided into 8 treatments with 4 replicates and ten chicks each. The experimental design was completely randomized ...
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To study the effects of different levels of surplus Jujube fruit (SJF) on growth performance parameters, carcass yield and some biochemical parameters, 320 Ross 308 broiler chicks were randomly divided into 8 treatments with 4 replicates and ten chicks each. The experimental design was completely randomized design based on 2×4 factorial trial using four levels of jujube ( 0, 2.5 , 5 and 7.5 % ) and two enzyme levels ( 0 , 0.2 % ). The birds were fed dietary treatments from 10 to 42 d in growth (11- 24days) and finisher periods (25-42 days). Body weight and feed intake and feed conversion ratio (FCR) were determined at the end of grower and finisher period. Data analysis indicates that main effects of jujube were significantly lower in birds fed 25 g/kg diet SJF as compared to control. Feed intake, body weight, body weight gain and FCR did not affect the interactions between SJF and enzyme at 24 days and 42 days (P>0.05). The birds fed 50 g/kg SJF had lower relative weight of pancreases and heart as compared to birds fed 25 g/kg SJF. The relative weight of liver and heart were affected by the interaction between Enzyme and SJF levels (P>0.05).The birds fed SJF had lower abdominal fat. The concentration of serum total cholesterol was decreased with increasing the levels of SJF. The concentration of triglycerides, HDL and LDL were not affectd by the levels of SJF or enzyme.The main effects of SJF at level of 50 g/kg were significantly reduced the activity of AST and ALT (liver enzymes) as compared to control diet. The 50g/kg of SJF were showed lower concentration of calcium as compared to 25 g/kg of jujube (P<0/05).Supplementation of enzyme to SJF based had not significant effects on performance or blood parameters. It is concluded that inclusion of SJF to broiler diets may be improve the percentage of abdominal fat, blood cholesterol without any side affect on performance parameters. The SJF may be increased the activity of liver enzyme in broilers.
S. H. Shojaei; Nazar Afzali; S. J. Hoseini vashan
Abstract
This study was conducted to evaluate the effects of different levels of butyric acid triglyceride and prebiotics on performance, egg quality traits and blood lipids of laying hens. A 2 × 4 factorial experiment with 4 levels of butyric acid triglyceride (0%, 0.15%, 0.30%, 0.45%) and 2 levels ...
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This study was conducted to evaluate the effects of different levels of butyric acid triglyceride and prebiotics on performance, egg quality traits and blood lipids of laying hens. A 2 × 4 factorial experiment with 4 levels of butyric acid triglyceride (0%, 0.15%, 0.30%, 0.45%) and 2 levels of prebiotic (0 and 0.15%) based a completely randomized design were conducted. One hundred and ninety-two 33-week-old (Hy Line- W36) hens were randomly divided to 8 dietary treatments, with 3 replicates of 10 each. The results of this study showed a significant difference in percentage of egg production, egg mass and feed conversion ratio among dietary treatments (P<0.05). So that the higher percentages of egg production, egg mass production were observed in diet contained 0.15 % prebiotic alone and diet contained 0.15% acid + 0.15% prebiotic. The diet contained 0.30% acid + 0.15% prebiotic had lower feed conversion ratio. The egg weight (60.94 g) and daily feed intake (91.77 g) did not affected by dietary treatments (P>0.05). The dietary treatment did not affect egg quality traits (P>0.05). The blood cholesterol, LDL, HDL and Triglyceride were not affected by dietary treatments (P>0.05). It is concluded that supplementation of 0.15% prebiotic alone and 0.15% butyric acid triglyceride and 0.15 prebiotics together may improve the laying performance without any effect on egg quality and blood lipids parameters
S.J Hoseini Vashan; A Golian; A Yaghobfar
Abstract
This experiment was conducted to evaluate the effects of turmeric powder (TRP), tomato pomace (TP), fat sources (canola oil, soybean oil and tallow) on immune system and jejunum morphometric parameters of heat stressed broiler chickens. Five hundred four one-d-old Ross broiler were randomly distributed ...
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This experiment was conducted to evaluate the effects of turmeric powder (TRP), tomato pomace (TP), fat sources (canola oil, soybean oil and tallow) on immune system and jejunum morphometric parameters of heat stressed broiler chickens. Five hundred four one-d-old Ross broiler were randomly distributed to 36 experimental units and 12 dietary treatments (3 replicates with 12 birds in each). The completely randomized design with factorial arrangement 3× 2× 2 (3 oils: canola, soybean, tallow, 2 TRP levels involved 0.4, 0.8% and 2 TP levels 3, 5%) were used. A daily heat stressed schedule (33oC for 5 h) was applied from 29 to 42d. At 28 and 42 d of age, two birds were bled and plasma and hemolysate were collected. The blood cholesterol at PHS and LDL-C were decreased and HDL-C were increased when birds fed canola and soybean oil. TRP diet decreased the LDL-C and increased HDL-C. Blood lipids were not affected by TP diets. Canola oil decreased heterophil:lymphocyte (H:L)ratio at PHS, however; TRP and TP decreased H:L ratio at AHS. The lower blood enzyme activity of ALP, AST and ALT were decreased in heat stressed birds fed canola oil as compared to tallow. TP also affect ALP and ALT activity. The higher activity of GPx and lower TBARS index were observed in birds fed canola oil or TRP diet. Therefore, addition of canola oil with TRP or TP could decrease the blood lipids, liver enzyme activity, the H:L ratio and improve the antioxidant status of broiler under heat stress.
N. Vatankhah; N. Afzali; S. J. Hoseini Vashan
Abstract
This experiment was performed to determine the role of vitamin E and coenzyme Q10 antioxidants on performance and ascites parameters of broiler chicks in cold stress conditions using a completely randomized design with 5 treatments and 5 replicates in each treatment. Treatments were: positive control ...
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This experiment was performed to determine the role of vitamin E and coenzyme Q10 antioxidants on performance and ascites parameters of broiler chicks in cold stress conditions using a completely randomized design with 5 treatments and 5 replicates in each treatment. Treatments were: positive control (PC, normal thermal conditions without antioxidants), negative control (NC, cold stress without antioxidants), NC + vitamin E (150 mg/kg diet), NC + Q10 (40 mg/kg diet) and NC + E (150 mg/kg diet) + Q10 (40 mg/kg diet). Temperature decreased gradually between days 15 to 21 and fixed at 15oC afterwards. Result showed that there were significant differences between treatments in body weight gain (BWG) and feed conversion ratio (FCR) (p<0.05). The NC group had the lowest BWG and the highest FCR amongst the dietary treatments but there were no significant difference between positive control and antioxidant group for this trait. In spite of a higher amount of FI for the negative control, there were no significant differences in FI between the treatments. Ascites indicators such as heart weight, RV/TV ratio, red blood cell count, hematocrit, and T3/T4 ratio were significantly increased in the negative control (p<0.05). Antioxidants in stressful conditions improved these parameters. Beside of an increase in liver weight and T3 were observed in the negative control but the differences were not significant (p<0.05). In general, the use of vitamin E and coenzyme Q10 antioxidants led to improvement in performance and ascites index in broiler chicken exposed to cold stress.
