نوع مقاله : مقاله پژوهشی
نویسندگان
1 موسسه تحقیقات علوم دامی کشور، سازمان تحقیقات آموزش و ترویج کشاورزی، کرج، ایران.
2 گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.
چکیده
هدف از این مطالعه بررسی اثر غلظت‌های مختلف کوآنزیم Q10بر انجمادپذیری اسپرم بز سانن بود. به این منظور از پنج راس بز بالغ نژاد سانن استفاده شد. پس از اسپرم‌گیری بوسیله واژن مصنوعی و ارزیابی اولیه، نمونه‌های منی در رقیق‌کننده تریس حاوی سطوح مختلف کوآنزیم Q10 (0، 1/0، 1، 10 و 100 میکرومولار) رقیق‌سازی و سپس منجمد شد. فراسنجه‌های حرکتی، سلامت غشاء، مورفولوژی، فعالیت میتوکندری، سلامت آکروزوم، غلظت مالون‌دی‌آلدئید و زنده‌مانی پس از فرایند انجماد و یخ‌گشایی مورد ارزیابی قرار گرفت. نتایج نشان داد استفاده از غلظت‌های 10 و 100 میکرومولار کوآنزیم Q10 به ترتیب بیشترین و کمترین اثر را بر حفظ فراسنجه‌های کیفی اسپرم در طول فرایند انجماد داشته است. به این ترتیب که بیشترین میزان تحرک کل، تحرک پیش‌رونده، سلامت غشاء، فعالیت میتوکندری، سلامت آکروزوم و زنده‌مانی اسپرم و کمترین میزان تولید مالون‌دی‌آلدهید به عنوان شاخصی از پراکسیداسیون لیپید مربوط به غلظت 10 میکرومولار کوآنزیم Q10 بود. بنابراین افزودن آنتی‌اکسیدان کوآنزیم Q10به رقیق‌کننده اسپرم روشی مناسب جهت حفظ فراسنجه‌های کیفی اسپرم منجمد‌شده بز می‌باشد.
کلیدواژه‌ها
موضوعات
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