تاثیر پرتوتابی بر ناپدید شدن شکمبه ای ماده خشک و پروتئین خام، و قابلیت هضم برون تنی کنجاله کانولا

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار، گروه علوم دامی، دانشکده کشاورزی و منابع طبیعی، دانشگاه گنبد کاووس

2 استاد، گروه تغذیه دام و طیور، دانشکده علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان

3 استادیار، پژوهشگاه علوم و فنون هسته ای، پژوهشکده کشاورزی هسته ای کرج

4 استادیار ، موسسه تحقیقات علوم دامی کشور

چکیده

این پژوهش به­منظور بررسی تاثیر پرتوهای یون­ساز گاما و الکترون در دزهای 25، 50 و 75 کیلوگری، بر تجزیه شکمبه­ای ماده خشک و پروتئین خام، و قابلیت هضم برون تنی کنجاله کانولا انجام گرفت. بدین منظور از تکنیک کیسه­های نایلونی و روش هضم دو مرحله­ای استفاده شد. پرتو گاما باعث کاهش تجزیه­پذیری ماده خشک شد (05/0P<)، اما پرتو الکترون تاثیری بر این صفت نداشت (05/0P>). تجزیه شکمبه­ای پروتئین خام به­وسیله پرتوتابی کاهش یافت (05/0P<). در این خصوص تاثیر اشعه گاما بیشتر از تابش الکترون بود (05/0P<). قابلیت هضم برون تنی کنجاله­ کانولا تحت تاثیر پرتوتابی افزایش یافت (05/0P<). با افزایش دز تابش پرتوهای الکترون و گاما، مقدار قابلیت هضم برون­تنی ماده خشک، به­طور خطی افزایش یافت (05/0P<). تاثیر تابش الکترون و اشعه گاما در افزایش این صفت یکسان بود (05/0P>). نتایج این پژوهش نشان داد که هرچند تاثیر پرتو گاما در کاهش تجزیه شکمبه ای ماده خشک و پروتئین خام کنجاله کانولا بیشتر از پرتو الکترون بود، اما قابلیت هضم برون تنی آن به طور یکسانی تحت تاثیر این پرتوهای یون­ساز افزایش یافت.  

1- Abu, J.O. Muller, K. Duodu, K.G. and Minnaar, A. (2006) Gamma irradiation of cowpea (Vigna unguiculata L. Walp) flours and pastes: Effects on functional, thermal and molecular properties of isolated proteins. Food Chem. 95, 138-147.
2- ASTM. (1984) Method for using the Fricke dosimeter to measure absorbed dose in water. ASTM Standard E 1026.
3- Chapelier, A. Desmadril, M. and Houe e-Levin, C. (2001) Gamma irradiation effects on α-lactalbumin: structural modifications. Can. J. Physiol. Pharmacol. 79, 154-157.
4- Cho, Y. Yang, J.S. and Song, K.B. (1999) Effect of ascorbic acid and protein concentration on the molecular weight profile of bovine serum albumin and b-lactoglobulin and c-irradiation. Food Res. Int. 32, 515-519.
5- Ciesla, K. Roos, Y. and Gluszewski, W. (2000) Denaturation processes in gamma irradiated proteins studied by differential scanning calorimetry. Radiat. Phys. Chem. 58, 233-243.
6- Dhali, A. Mishra, D.P. Mehla, R.K. and Sirohi, S.K. (2006) Usefulness of milk urea concentration to monitor the herd reproductive performance in crossbred karanfries cows. Asian-Aust. J. Anim. Sci. 19, 26-30.
7- Ebrahimi, S.R. Nikkhah, A. Sadeghi, A.A. and Raisali, G. (2009) Chemical composition, secondry compounds, ruminal degradation and in vitro crude protein digestibility of gamma irradiated canola seed. Anim. Feed Sci. Technol. 151, 184-193.
8- Ebrahimi-Mahmoudabad, S.R. and Taghinejad-Roudbaneh, M. (2011) Investigation of electron beam irradiation effects on anti-nutritional factors, chemical composition and digestion kinetics of whole cottonseed, soybean and canola seed. Radiat. Phys. Chem. 80, 1441-1447.
9- El-Hakim, N.F. Yousri, R.M. Roushdy, H. and Farag, M.D. (1991) Nutritional evaluation of irradiated animal protein by-products. Isotopenpraxis. 27, 104-108.
10- El-Shazali, A.M. Nahid, A.A. Salma, H.A. and El-Fadil, E.B. (2011) Effect of radiation process on antinutrients, protein digestibility and sensory quality of pearl millet flour during processing and storage. Int. Food Res. J. 18, 1401-1407.
11- Fintzou, A.T. Kontominas, M.G. Badeka, A.V. Stahl, M.R. and Riganakos, K.A. (2007) Effect of electron beam and gamma-irradiation on physicochemical and mechanical properties of polypropylene syringes as a function of irradiation dose: Study under vacuum. Radiat. Phys. Chem. 76, 1147-1155.
12- Forooshani, MJ. (2010) Effect of electron beam irradiation on dry matter and crude protein degradability of soybean and canola meal and performance of lactating Holstein cows. Degree Diss., Univ. IUT, Iran.
13- Gaber, M.H. (2005) Effect of g-irradiation on the molecular properties of bovine serum albumin. J. Biosci. Bioeng. 100, 203-206.
14- Gharaghani, H. Zaghari, M. shahhoseini, G. and Moravej, H. (2008) Effect of gamma irradiation on antinutritional factors and nutritional value of canola meal for broiler chickens. Asian–Aust. J. Anim. Sci. 21, 1479-1485.
15- Koksel, H. Sapirstein, H.D. Celik, S. and Bushuk, W. (1998) Effects of gamma irradiation of wheat on gluten proteins. J. Cereal Sci. 28, 243-250.
16- Kopecny, J. and Wallace, R.J. (1982) Cellular location and some properties of proteolytic enzymes of rumen bacteria. Appl. Environ. Microbiol. 43, 1026-1033.
17- Kridli, R.T. Haddad, S.G. and Muwalla, M.M. (2001) The effect of feeding ruminally undegradable protein on postpartum reproduction of Awassi Ewes. Asian-Aust. J. Anim. Sci. 14, 1125-1128.
18- Lee, J.W. Kim, J.H. Yook, H. S. Kang, K.O. Lee, S.Y. Hwang, H.J. and Byun, M.W. (2001) Effect of gamma irradiation on the allergenic and antigenic properties of milk proteins. J. Food Prot. 64, 272-276.
19- Lee, S.L. Lee, M.S. and Song, K.B. (2005) Effect of gamma-irradiation on the physicochemical properties of gluten films. Food Chem. 92, 621-625.
20- Maesoomi, S.M. Ghorbani, G.R. Alikhani, M. and Nikkhah, A. (2006) Canola meal as a substitute for cottonseed meal in diet of midlactation Holsteins. J. Dairy Sci. 89, 1673-1677.
21- Murray, K. Granner, D.K. Mayes, P.A. and Rodwell, V.W. (2003) Harper’s Biochemistry, 26th ed. McGraw-Hill, New York, NY, USA.
22- Orskov, E.R. and McDonald, I. (1979) The estimation of protein degradability in the rumen from incubation measurements weighted according to rumenrate of passage. J. Agric. Sci. Camb. 92, 499-503.
23- Salamatdoust-Nobar, R. Chamani, M. Sadeghi, A.A. and Aghazadeh, A.M. (2009) Determination of degradability of treated soybean meal and its protein fractions. Afr. J. Biotechnol. 8, 98-102.
24- Sandrock, C.M. Armentano, L.E. Thomas, D.L. and Berget, Y.M. (2009) Effect of protein degradability on milk production of dairy ewes. J. Dairy Sci. 92, 4507-4513.
25- SAS. (2003) SAS User’s Guide: Statistics, Version 9.1 Edition.SAS Institute, Cary, NC, USA.
26- Shawrang, P. Mansouri, M.H. Sadeghi, A.A. and Ziaie, F. (2011) Evaluation and comparison of gamma and electron beam irradiation effects on total and free gossypol of cottonseed meal. Radiat. Phys. Chem. 80, 761-762.
27- Shawrang, P. Nikkhah, A. Zare-Shahneh, A. Sadeghi, A.A. Raisali, G. and Moradi-Shahrbabak, M.M. (2008) Effects of gamma irradiation on chemical composition and ruminal protein degradation of canola meal. Radiat. Phys. Chem. 77, 918-922.
28- Siddhuraju, P. Makkar, H.P.S. and Becker, K. (2002) The effect of ionizing radiation on antinutritional factors and the nutritional value of plant materials with reference to human and animal food. Food Chem. 78, 187-205.
29- Taghinejad- Roudbaneh, M. Ebrahimi, S.R. Azizi, S. and Shawrang, P. (2010) Effect of electron beam irradiation on chemical composition, antinutritional factors, ruminal degradation and in vitro protein digestibility of canola meal. Radiat. Phys. Chem. 79, 1264-1269.
30- Taghinejad, M. Nikkhah, A. Sadeghi, A.A. Raisali, G. and Chamani, M. (2009) Effects of gamma irradiation on chemical composition, antinutritional factors, ruminal degradation and in vitro protein degradability of full-fat soybean. Asian-Aust. J. Anim. Sci. 22, 534-541.
31- Tilly, J.M.A. and Terry, R.A. (1963) A two stage technique for the in vitro digestion of forage crops. J. Br. Grassland Soc. 18, 104-111.
32- Tuncer, S.D. and Sacakli, P. (2003) Rumen degradability characteristics of xylose treated canola and soybean meals. Anim. Feed Sci. Technol. 107, 211-218.
33- Wright, C.F. (1995) The evaluation of heat and lignosulfonate treated canola meal and sources of rumen undegradable protein for lactating cows. B. Sc. Thesis. University of British Columbia. 103p