Effects of Vitamin E and Arginine supplementations on performance, enzyme activist and blood parameters in broiler with pulmonary hypertension syndrome (Ascites).

Document Type : Research Paper

Authors

Abstract

320 one-day male broilers (Ross 308) were divided in 4 treatments (with 4 replicate and 20 chicks for a replicate). All of broilers reared under a cold environmental temperature to induce Ascites .Experimental treatments were included 1: was fed a commercial corn-soybean meal- based diet meeting NRC (1994) including 1.2% Arg and 40 IU of Vitamin E/kg (Control), 2: Control with 400 IU of Vitamin E/kg of feed 3: Control with 0.3% Arg in water and 4: combination of both Vitamin E and Arg. At d 14, temperature was reduced to amplify the incidence of Pulmonary Hypertension.  Body weight gain, feed intake and feed conversion ratio were measured weekly from week 3. Hematological, biochemical and pathological tests were measured at day 21 and 42; total red blood cell, hemoglobin, hematocrit, activity of alanine transaminase, aspartate transaminase, lactate dehydrogenase. At 42 days of age, 2 chicks from each replicate were randomly selected and slaughtered and then ratio of right ventricle weight to total ventricle weight calculated.The our results showed that Arg treatment had the highest body weight gain and the lowest feed conversion ratio in total period. Red blood cell, hematocrit, hemoglobin and plasma level protein was significantly lower in Arg and Arg- Vitamin E treatments compared to other group at d 42. Plasma protein was lower in Arg- Vitamin E treatment compared to control treatment.  Also, lactate dehydrogenase activity was significantly lower in Arg group and alanine transaminase activity was significantly higher in Arg- Vitamin E group at d 21. Moreover, ratio of right ventricle weight to total ventricle and mortality due to Ascites, were significantly lower in Arg group

References

1-    Arab, H.A R. Jamshidi, A. Rassouli, G. Shams and Hassanadeh. M.H. (2006) Generation of hydroxyl radicals during ascites experimentally. British Poultry Science Volume 47, Number 2pp. 216—222.
2-    Bottje, W.B. & Wideman, Jr. R.F. (1995) Potential role of free radicals in the pathogenesis of pulmonary hypertension syndrome. Poult. Avian Biol. Rev., 6, 221-231.
3-    Bottje, W.G., Erf, G.F., Bersi, T.K., Wang, S., Barnes, D. and Beers, K.W. (1997) Effect of dietary dl-a tocopherol on tissue a- and g-tocopherol and pulmonary hypertension syndrome (ascites) in broilers. Poult. Sci., 76, 1506- 1512.
4-    Bowry, V. W., and R. Stocker .(1993) Tocopherol-mediated peroxidation: the prooxidant effect of vitamin E on the radical initiated oxidation of human low density lipoprotein. J. Am. Chem. Soc. 115: 6029–6040.
5-    Buys, N., J. Buyse, M. Hassanzadeh-Ladmakhi, and E. Decuypere(.1998) Intermittent  lighting reduces the incidence of ascites in broilers: An interaction with protein content of feed on performance and the endocrine system. Poult. Sci. 77: 54- 61.
6-    Daneshyar M, Kermanshahi H. and Golian. A. (2009) Changes of biochemical parameters and enzyme activities in broiler chickens with cold-induced ascites. Poultry Science 88:106–110.
7-    Enkvetchakul, B., Bottje, W. Anthony, N. Moore, R. and Huff W. (1993) Compromised antioxidant status associated with ascites in broilers. Poultry Sci. 72:2272–2280.
8-    Druyan, S., A. Ben-David, and A. Cahaner. 2007. Development of ascites-resistant and ascites-susceptible broiler lines. Poult. Sci. 86:811–822.
9-    Druyan, S., D. Shinder , A. Shlosberg , A. Cahaner , and S. Yahav. (2009) Physiological parameters in broiler lines divergently selected for the incidence of ascites. Poult. Sci. 88:1984–1990.
10-                            Fathi. M, Nazer Adl, K. Ebrahim Nezhad, Y. Aghdam Shahryar, H. Daneshyar M. and , Tanha. T. (2011) The role of oxidative stress in the development of congestive heart failure (CHF) in broilers with pulmonary hypertension syndrome (PHS). Journal of Animal and Veterinary advance.10(20): 2722-2719.
11-    Fathi. M, Tanha, T. Nazer Adl, K. Ebrahim Nezhad, Y. Aghdam Shahryar, H. and Daneshyar. M. (2012) Plasma Enzymes activities and antioxidant status characterization in pulmonary hypertension syndrome (PHS) in broilers. African Journal of Agricultural Research Vol. 7(5), pp.1414-1419.
12-    Geng. A. L., Guo, Y. M. and Yang. Y. (2004) Reduction of ascites mortality in broilers by coenzyme Q10. Poultry Science 83:1587–1593.
13-    Jorens, P. G., P. A. Vermiere, and A. G. Herman. (1993) L-arginine- dependent nitric oxide synthase: A new metabolic pathway in the lung and airways. Eur. Respir. J. 6:258 266.
14-    Iqbal., M., Cawthon, D. Wideman, R.F. Jr. and Bottje, W.G. (2001) Lun g mitochondrial dysfunction in pulmonary hypertension synd rome I. Site specific defects in electron transport chain. Poult. Sci., 80, 485-495.
15-    Lorenzoni, A. G., and Ruiz-Feria. C.A. (2006) Effects of vitamin E and l-arginine on Cardiopulmonary function and ascites parameters in broilers chickens reared under sub-normal temperatures. Poult. Sci. 85:2241–2250.
16-    Luger, D., D. Shinder, V. Rzepakovsky, M. Rusal, and S. Yahav. (2001). Association between weight gain, blood parameters, and thyroid hormones and the development of ascites syndrome in broiler chickens. Poult. Sci. 80:965- 971.
17-    Nain, S. B., B. Ling, J. Bandy, C. Alcorn, B. Wojnarowicz, B. Laarveld and A. A. Olkowski. (2008) The role of oxidative stress in the development of congestive heart failure in a chicken genotype selected for rapid growth. Avian Pathology. 37(4):367_373.
18-    Ruiz-Feria, C. A., M. T. Kidd and R. F. Wideman. (2001) Plasma Levels of Arginine, Ornithine, and Urea and Growth Performance of Broilers Fed Supplemental L-Arginine during Cool Temperature Exposure. Poult. Sci. 80:358–369.
19-    Ruiz-Feria. C.A., (2009) Concurrent supplementation of arginine, vitamin E, and vitamin C improve cardiopulmonary performance in broilers chickens. Poultry Science 88:526–535.
20-    Scheele, C. W., W. De Wit, M. T. Frankenhuis, and P.F.G .Vereijken. (1991) Ascites  in broilers. Experimental factors evoking symptoms related to ascites. Poult. Sci. 70:1069- 1083.
21-    Wideman, R.F. Jr. (1988) Ascites in poultry. Monsanto Nutr. Update 6:1-7.
22-    Wideman, R. F., Y. K. Kirby, M. Ismalil, W. Bottje, R. Moore, and R. Vardeman. (1995) Supplemental l-arginine attenuates pulmonary hypertension syndrome (ascites) in broilers. Poult. Sci.74:323–330.
23-    Wideman, R.F. Jr. (1998) Causes and control of ascites in b roilers. National Meeting on Poultry Health and Processing, 33, 56 - 85.
24-    Wideman, R.F. Jr. and C. D. Tackett. (2000) Cardio-pulmonary function in broiler reared at warm or cool temperatures: effect of acute inhalation of 100% oxygen.  Poult. Sci. 79:257- 264.
25-    Yersin, A. G., W. E. Huff, L. F. Kubena, M. H. Elissalde, R. B. Harvey, D. A. Witzel and L. E. Giroir. (1992) Changes in hematological, blood gas and serum biochemical  l variables

Files

Share

How to cite

Statistics