بررسی اثر گیاه دارویی خار مریم بر اندازه قطر هسته و شکست DNA اسپرم تیمار شده با کلرید کادمیوم در قوچ نژاد فراهانی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد گروه زیست شناسی دانشکده علوم پایه دانشگاه اراک.

2 دانشیارگروه علوم دامی دانشکده کشاورزی و منابع طبیعی دانشگاه اراک.

3 دانشیار گروه زیست شناسی دانشکده علوم پایه دانشگاه اراک.

چکیده

اسپرم‌ مورد استفاده در مطالعه حاضر از اپیدیدیم بیضه‌های قوچ‌های فراهانی کشتار شده جمع‌آوری و به چهار گروه تقسیم شد. در هر نمونه، 106× 5 اسپرم وجود داشت. تیمارهای آزمایشی شامل اسپرم‌های لحظه صفر، اسپرم‌های لحظه 180 دقیقه (شاهد)، اسپرم‌های تیمار شده با کلرید کادمیوم (10 میکرومولار) به مدت 180 دقیقه و اسپرم‌های تیمار شده با سیلیمارین (5 میکرومولار) همراه با کلرید کادمیوم (10 میکرومولار) به مدت 180 دقیقه بودند. برای بررسی جنبه‌های مورفولوژیکی آپوپتوز در هسته اسپرم از رنگ‌آمیزیDiff-Quick استفاده گردید. همچنین برای برآورد شکست DNA از رنگ‌آمیزی اکریدین اورنژ و تست SCD استفاده شد. آنالیز داده‌ها از طریق روش تجزیه واریانس یک طرفه صورت گرفت. مقایسه میانگین تیمارها با استفاده از آزمون توکی در سطح خطای 05/0 انجام شد. در این پژوهش، قطر هسته اسپرم در گروه تیمار شده با کلرید کادمیوم نسبت به گروه شاهد به طور معنی‌داری (05/0P<) کاهش یافت. استفاده از سیلیمارین همراه با کلرید کادمیوم توانست کاهش قطر هسته اسپرم را نسبت به گروه تیمار شده با کلرید کادمیوم به طور معنی‌داری جبران کند. همچنین نتایج نشان دادند که درصد شکست DNA در گروه تیمار شده با کلرید کادمیوم در مقایسه با گروه شاهد (001/0>P) و گروه تیمار شده با سیلیمارین همره با کلرید کادمیوم (01/0>P) به طور معنی‌داری افزایش یافت

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