بررسی تاثیر عصاره گیاه آنیسون(Pimpinella anisum L.) و گلپر(Heracleum persicum) در مقایسه با اکسی‌تتراسایکلین و پروبیوتیک پریمالاک بر پاسخ ایمنی، جمعیت میکروبی ایلئوم و خصوصیات مورفولوژیکی تهی‌روده جوجه‌های گوشتی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری تغذیه دام دانشگاه آزاد اسلامی واحد اراک

2 استادیار گروه علوم دامی دانشگاه آزاد اسلامی واحد اراک.

3 استاد پژوهشی موسسه تحقیقات علوم دامی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران

4 استادیار گروه علوم دامی دانشگاه آزاد اسلامی واحد اراک

چکیده

هدف از این آزمایش تعیین اثرات عصاره‌های گیاهان آنیسون و گلپر(mg/kg 200) در مقایسه با آنتی‌بیوتیک اکسی‌تتراسایکلین(mg/kg 200) و پروبیوتیک پریمالاک(mg/kg 100)بر پاسخ ایمنی، جمعیت میکروبی ایلئوم و خصوصیات مورفولوژیکی تهی‌روده جوجه‌های گوشتی بود. تعداد 400 قطعه جوجه گوشتی سویه راس 308 در قالب طرح کاملاً تصادفی به پنج تیمار آزمایشی اختصاص داده شدند. هر تیمار شامل 4 تکرار و هر تکرار دارای 20 قطعه جوجه یک روزه بود. ارزیابی پاسخ ایمنی جوجه‌ها به کمک تزریق سوسپانسیون 5 درصد گلبول قرمز گوسفند(SRBC)، بررسی جمعیت میکروبی روده با برداشتن محتویات ایلئوم پرنده‌ها و بررسی خصوصیات مورفولوژیکی روده با بریدن قسمت میانی تهی‌روده پس از کشتار صورت گرفت. نتایج این مطالعه نشان دادند که تیمارهای آزمایشی تاثیر معنی‌داری بر عیار آنتی‌بادی علیه SRBC نداشتند. فراوانی هتروفیل‌ها و نسبت هتروفیل به لنفوسیت، تحت تاثیر تیمارهای آزمایشی عصاره گیاهان آنیسون و گلپر کاهش معنی‌داری نشان دادند(05/0>P). جمعیت کل باکتری‌های هوازی، اشریشیاکلی و کلی‌فرم‌ها تحت تاثیر عصاره گیاه گلپر کاهش معنی‌داری داشتند(05/0>P). کلیه تیمارهای حاوی افزودنی در مقایسه با تیمار شاهد سبب افزایش معنی‌داری در ارتفاع پرزهای تهی‌روده شدند(05/0>P). نسبت طول پرز به عمق کریپت در پرزهای تهی‌روده توسط تیمارهای حاوی عصاره گیاه آنیسون و گلپر به طور معنی‌داری افزایش یافت(05/0>P). به طور کلی نتایج حاصل از این آزمایش نشان دادند که استفاده از عصاره گیاه آنیسون و گلپر به میزان 200 میلی‌گرم در هر کیلوگرم جیره ممکن است باعث بهبود پاسخ ایمنی، کاهش جمعیت میکروبی مضر ایلئوم و بهبود خصوصیات مورفولوژیکی تهی‌روده جوجه های گوشتی شود.

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