نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار- مجتمع آموزش عالی تربت جام – دانشکده کشاورزی- گروه علوم دامی.

2 استاد- دانشگاه فردوسی مشهد- دانشکده کشاورزی- گروه علوم دامی.

3 استادیار- دانشگاه فردوسی مشهد- دانشکده کشاورزی- گروه علوم دامی.

چکیده

دمای بالا یکی از فاکتورهای اصلی محیطی مؤثر بر کاهش سود اقتصادی واحدهای پرورش طیور است، زیرا رشد و تولید پرنده را مختل می‎کند. پروتئین‎های شوک گرمایی (HSPs) نقش کلیدی در مکانیسم دفاعی سلولی علیه گرمای محیط دارند. هدف از انجام مطالعه حاضر، بررسی میزان بیان ژن‎های HSPA2 و HSPB1 در کبد مرغان بومی خراسان تحت تنش گرمایی حاد بود. برای این هدف، تعداد 16 قطعه جوجه در سن 42 روزگی به دو گروه شاهد (دمای C 25 و رطوبت %50) و تیماری (دمای 40 و C 42 و رطوبت %50 به مدت 24 ساعت) تقسیم شده و پس از کشتار از کبد آن‎ها نمونه‎برداری به عمل آمد. سپس میزان بیان ژن‎های HSPA2 و HSPB1 با روش RT-qPCR مورد ارزیابی قرار گرفت. بیان ژن HSPA2 نسبت به گروه شاهد افزایش معنی‎داری داشت (05/0  P)، در حالی که برای ژن HSPB1 افزایش معنی‎داری مشاهده نشد. آنالیز شبکه پروتئینی وجود برهم‎کنش پروتئینی بین HSPA2 و HSPB1 نشان نداد ولی بر اساس آماره FDR در هستی‎شناسی ژن چهار مسیر فرایند پروتئینی در شبکه آندوپلاسمی، بلوغ اووسیت وابسته به پروژسترون، و مسیرهای علامت دهی MAPK و VEGF بیشترین معنی‎داری را داشتند. عدم وجود تلفات و همچنین نتایج آنالیز بیان ژن نشان می‎دهند که مرغان بومی خراسان توانایی مقاومت در برابر تنش گرمایی را دارند و نسبت به تنش گرمایی حاد واکنش ژنی نشان می‎دهند، و از طرفی ژن HSPA2 توانایی بیان در این شرایط محیطی را به جهت حفظ ساختار پروتئینی درون سلولی دارد.

کلیدواژه‌ها

نصیری، م.ر. و رودباری، ز. (1393). تجزیه و تحلیل ژنتیکی ناحیه سیتوکروم b در مرغ بومی خراسان. مجله بیوتکنولوژی کشاورزی. 6:  198-189.
Abhinand, C.S., Raju, R., Soumya, S.J., Arya, P.S., Sudhakaran, P.R. (2016). VEGF-A/VEGFR2 signaling network in endothelial cells relevant to angiogenesis. Journal of cell communication and signaling. 10: 347-354.
Ali, N. and Banu, N. (1991). Heat Shock Proteins: Molecular Chaperones.  Biochemical Education. 19: 166-172.
Capaldi, A.P., Kaplan, T., Liu, Y., Habib, N., Regev, A., Friedman, N., et al. (2008). Structure and function of a transcriptional network activated by the MAPK Hog1. Nature Genetics. 40:1300–6.
Causton, H.C., Ren, B., Koh, S.S., Harbison, C.T., Kanin, E., Jennings, E.G., et al.  (2001). Remodeling of yeast genome expression in response to environmental changes. Molecular Biology of the Cell. 12: 323-337.
Cedraz, H., Gromboni, J.G.G., Garcia, A.A.P.J., Farias Filho, R.V., Souza, T.M., et al. (2017). Heat stress induces expression of HSP genes in genetically divergent chickens. PLOS ONE 12: e0186083.https://doi.org/10.1371/journal.pone.0186083.
Coble, D.J., Fleming, D., Persia, M.E., Ashwell, C.M., Rothschild, M.F., Schmidt, C.J., Lamont, S.J. (2014). RNA-seq analysis of broiler liver transcriptome reveals novel responses to high ambient temperature. BMC Genomics.15:1084.
Cvoro, A., Dundjerski, J., Trajkovic, D., Matic, G. (1998). Heat stress affects the glucocorticoid receptor interaction with heat shock protein Hsp70 in the rat liver. Biochemistry and Molecular Biology International. 46: 63-70.
De Nadal, E., Ammerer, G., Posas, F. (2011). Controlling gene expression in response to stress. Nature Reviews Genetics. 12: 833-45.
Duangjinda, M., Tunim, S., Duangdaen, C., Boonkum, W. (2017). Hsp70 genotypes and heat tolerance of commercial and native chickens reared in hot and humid conditions. Brazilian Journal of Poultry Science. 19: 7-18.
Figueiredo, D., Gertler, A., Cabello, G., Decuypere, E., Buyse, J., Dridi, S. (2007). Leptin downregulates heat shock protein-70 (HSP-70) gene expression in chicken liver and hypothalamus. Cell Tissue Research. 329: 91-101.
Gasch, A.P., Spellman, P.T., Kao, C.M., Carmel-Harel, O., Eisen, M.B., Storz, G., et al. (2000). Genomic expression programs in the response of yeast cells to environmental changes. Molecular Biology of the Cell. 11: 4241-57.
Golan, S., Entin-Meer, M., Semo, Y., Maysel-Auslender, S., Mezad-Koursh, D., Keren, G., et al. (2014). Gene profiling of human VEGF signaling pathways in human endothelial and retinal pigment epithelial cells after anti VEGF treatment. BMC research notes. 7: 617.
Huang, S. (2017). Upregulation of TLR4 mRNA expression levels in broiler chickens under acute heat stress. Brazilian Journal of Poultry Science. 19: 87-94.
Hwang, Y.S., Ko, M.H., Kim, Y.M., Park, Y.H., Ono, T., Han, J.Y. (2016). The avian-specific small heat shock protein HSP25 is a constitutive protector against environmental stresses during blastoderm dormancy. Scientific Reports. 6: 36704.
Ju, X.H., Xu, H.J., Yong, Y.H., An, L.L., Jiao, P.R., Liao, M. (2014). Heat stress upregulation of toll-like receptors 2/4 and acute inflammatory cytokines in peripheral blood mononuclear cell (PBMC) of bama miniature pigs:an in vivo and in vitro study. Animal. 8: 1462-1468.
Kang, D., Park, J., Shim, K. (2019). Heat Treatment at an Early Age Has Effects on the Resistance to Chronic Heat Stress on Broilers. Animals (Basel), 9(12).
Lan, X., Hsieh, J.C.F., Schmidt, C.J., Zhu, Q., Lamont, S.J. (2016). Liver transcriptome response to hyperthermic stress in three distinct chicken lines. BMC Genomics. 17: 955.
Liu, Y., and Chang, A. (2008). Heat shock response relieves ER stress. The EMBO journal. 27: 1049-1059.
Miller, C., Schwalb, B., Maier, K., Schulz, D., Du¨mcke, S., Zacher, B., et al. (2011). Dynamic transcriptome analysis measures rates of mRNA synthesis and decay in yeast. Molecular Systems Biology. 7:458.
Ogata, T., Oishi, Y., Roy, R.R., Ohmori, H. (2005). Effects of T3 treatment on HSP72 and calcineurin content of functionally overloaded rat plantaris muscle. Biochemical and Biophysical Research Communications. 331: 1317-1323.
Quinn, C.M., Audet, G.N., Charkoudian, N., Leon, L.R. (2015). Cardiovascular and thermoregulatory dysregulation over 24 h following acute heat stress in rats. American Journal of Physiology Heart & Circulatory Physiology. 309: 91-95.
Richter, C., Viergutz, T., Schwerin, M., Weitzel, J.M. (2015). Prostaglandine synthase interacts with inducible heat shock protein 70 after heat stress in bovine primary dermal fbroblast cells. Cytometry Part A. 87: 61-67.
Rimoldi, S., Lasagna, E., Sarti, F.M., Marelli, S.P., Cozzi, M.C., Bernardini, G., and Terova, G. (2015). Expression profile of six stress-related genes and productive performances of fast and slow growing broiler strains reared under heat stress conditions, Meta Gene. 6: 17-25.
Roushdy, E.M., Zaglool, A.W., El-Tarabany, M.S. (2018). Effects of chronic thermal stress on growth performance, carcass traits, antioxidant indices and the expression of HSP70, growth hormone and superoxide dismutase genes in two broiler strains. Journal of Thermal Biology. 74: 337-343.
Sonna, L.A., Fujita, J., Gaffin, S.L., Lilly, C.M. (2002). Invited review: effects of heat and cold stress on mammalian gene expression. The Journal of Applied Physiology. 92: 1725-1742.
Saretzki, G., Walter, T., Atkinson, S., Passos, J.F., Bareth, B., Keith, W.N., Stewart, R., Hoare, S., Stojkovic, M., Armstrong, L., von Zglinicki, T., Lako, M. (2008). Downregulation of multiple stress defense mechanisms during differentiation of human embryonic stem cells. Stem Cells. 26: 455–464.
Soleimani, A.F., Zulkifli, I., Omar, A.R., Raha, A.R. (2011). Physiological responses of 3 chicken breeds to acute heat stress. Poultry Science. 90: 1435-40.
Staib, J.L., Quindry, J.C., French, J.P., Criswell, D.S., Powers, S.K. (2007). Increased temperature, not cardiac load, activates heat shock transcription factor 1 and heat shock protein 72 expression in the heart. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 292: 432–439.
Sun, L., Lamont, S.J., Cooksey, A.M., Mccarthy, F., Tudor, C.O., Vijay-Shanker, K., et al. (2015). Transcriptome response to heat stress in a chicken hepatocellular carcinoma cell line. Cell Stress & Chaperones. 20: 1-12.
Temim, S., Chagneaua, M., Peresson, R., Tesseraud, S. (2000). Chronic heat exposure alters protein turnover of three different skeletal muscles in finishing broiler chickens fed 20 or 25% protein diets. The Journal of Nutrition. 130: 813–9.
Vinoth, A., Thirunalasundari, T., Tharian, J.A., Shanmugam, M., Rajkumar, U. (2015). Effect of thermal manipulation during embryogenesis on liver heat shock protein expression in chronic heat stressed colored broiler chickens. Journal of Thermal Biology. 53: 162-171.
Whiteley, E.M. and Betenbaugh, M.J. (2003). Protein processing, processing in the Endoplasmic Reticulum and Golgi network. In Encyclopedia of Cell Technology, R.E. Spier (Ed.). doi:10.1002/0471250570.spi095.
Xie, J., Tang, L., Lu, L., Zhang, L., Xi, L., Liu, H.C., Odle, J., Luo, X. (2014). Differential Expression of Heat Shock Transcription Factors and Heat Shock Proteins after Acute and Chronic Heat Stress in Laying Chickens (Gallus gallus). PLoS One. 29: 9:e102204. doi: 10.1371/journal.pone.0102204. eCollection.
Xu, Y., Lai, X., Li, Z., Zhang, X., Luo, Q. (2018). Effect of chronic heat stress on some physiological and immunological parameters in different breed of broilers. Poultry Science. 97: 4073–4082.
Zhang, W.W., Kong, L.N., Zhang, X.Q., Luo, Q.B. (2014). Alteration of HSF3 and HSP70 mRNA expression in the tissues of two chicken breeds during acute heat stress. Genetics and Molecular Research. 13: 9787-9794.