نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار پژوهشی پژوهشکده دامهای خاص پژوهشگاه زابل

2 استادیار، گروه علوم دامی، دانشکده کشاورزی و منابع طبیعی، دانشگاه خلیج فارس، بوشهر، ایران

چکیده

پژوهش حاضر به‌ منظور ارزیابی اثرات سین‌بیوتیک بر عملکرد، صفات کیفی تخم‌مرغ، جمعیت میکروبی و ریخت‌سنجی روده کوچک مرغ‌های تخم‌گذار در شرایط تنش مزمن گرمایی و برآورد نقطه بهینه مصرف آن با استفاده از مدل‌سازی خط شکسته انجام شد. این آزمایش در قالب طرح کاملاً تصادفی با چهار تیمار، چهار تکرار و نه قطعه‌ مرغ در هرتکرار انجام شد. تیمارها شامل سطوح صفر(شاهد) 5/0، 1 و 5/1 گرم در کیلوگرم سین‌بیوتیک بود که به جیره پایه اضافه شد. یافته‌ها نشان داد که افزودن سین-بیوتیک، درصد تخم‌گذاری، توده تخم‌مرغ و وزن تخم‌مرغ را در 42-44 هفتگی و 44-46 هفتگی افزایش داد )05/0(p<. همچنین توده تخم مرغ و درصد تخم‌گذاری در همه گروه‌های دریافت‌کننده سین‌بیوتیک در مقایسه با شاهد، در کل دوره بیشتر بود05/0(p<. کمترین و بیشترین مقدار توده تخم‌مرغ در کل دوره مربوط به گروه شاهد و گروه دریافت‌کننده 5/0 گرم سین‌بیوتیک بود(به ترتیب 88/45 و 6/49). شمار سالمونلا در گروه‌های 5/0، 1 و 5/1 گرم سین‌بیوتیک در مقایسه با شاهد کمتر شد )05/0(p<. برآورد سطح بهینه در جیره بر اساس مدل‌سازی خط شکسته خطی و درجه دو برای درصد تخم‌گذاری به ترتیب 93/0 و 05/1 گرم سین‌بیوتیک در کیلوگرم جیره بود. در مجموع، استفاده از سین-بیوتیک باعث بهبود وزن تخم مرغ، شاخص تولید و درصد تخم‌گذاری همراه با کاهش جمعیت سالمونلا شد و مقدار یک گرم در کیلوگرم برای شرایط تنش گرما توصیه می‌گردد.

کلیدواژه‌ها

موضوعات

Abdelqader, A., Al-Fataftah, A. R., & Daş, G. (2013). Effects of dietary Bacillus subtilis and inulin supplementation on performance, eggshell quality, intestinal morphology and microflora composition of laying hens in the late phase of production. Animal Feed Science and Technology179(1-4), 103-111. . https://doi.org/10.1128/AEM.00600-
Abdel-Wareth, A. A. A. (2016). Effect of dietary supplementation of thymol, synbiotic and their combination on performance, egg quality and serum metabolic profile of Hy-Line Brown hens. British poultry Science57(1), 114-122. doi.org/10.1080/00071668.2015.1123219
Akbarian, A., Golian, A.H., Kermanshahi, R, Farhoosh, A.R., Raji, S. (2013). Growth performance and gut health parameters of finishing broilers supplemented with plant extracts and exposed to daily increased temperature. Spanish Journal Agricalture Research. 11:109-119. doi.org/10.5424/sjar/2013111-3392
Bozkurt, M., Küçükyilmaz, K., Catli, A. U., Çınar, M., Bintaş, E., & Çöven, F. (2012). Performance, egg quality, and immune response of laying hens fed diets supplemented with mannan-oligosaccharide or an essential oil mixture under moderate and hot environmental conditions. Poultry science91(6), 1379-1386. https://doi.org/10.3382/ps.2011-02023
Brenes, A., & Roura, E. (2010). Essential oils in poultry nutrition: Main effects and modes of action. Animal Feed Science and Technology158(1-2), 1-14. doi:10.1016/j.anifeedsci.2010.03.007
Burkholder, K. M., Thompson, K. L., Einstein, M. E., Applegate, T. J., & Patterson, J. A. (2008). Influence of stressors on normal intestinal microbiota, intestinal morphology, and susceptibility to Salmonella enteritidis colonization in broilers. Poultry Science87(9), 1734-1741. https://doi.org/10.3382/ps.2008-00107
Deng, W., Dong, X. F., Tong, J. M., & Zhang, Q. (2012). The probiotic Bacillus licheniformis ameliorates heat stress-induced impairment of egg production, gut morphology, and intestinal mucosal immunity in laying hens. Poultry Science91(3), 575-582. https://doi.org/10.3382/ps.2010-01293
Dunislawska, A., Slawinska, A., Stadnicka, K., Bednarczyk, M., Gulewicz, P., Jozefiak, D., & Siwek, M. (2017). Synbiotics for broiler chickens—in vitro design and evaluation of the influence on host and selected microbiota populations following in ovo delivery. PloS one12(1), e0168587. doi.org/10.1371/journal.pone.0168587
Freestone, P. P., Sandrini, S. M., Haigh, R. D., & Lyte, M. (2008). Microbial endocrinology: how stress influences susceptibility to infection. Trends in Microbiology16(2), 55-64. https://doi.org/10.1016/j.tim.2007.11.005
Haugh RR (1937) The Haugh unit for measuring egg quality. U.S. Egg Poultry 43: 552-555 & 572-573.
Jahromi, F., Wesam Altaher, Y., Shokryazdan, P., Ebrahimi, R., Ebrahimi, M., Idrus, Z., ... & Liang, J. B. (2016). Dietary supplementation of a mixture of Lactobacillus strains enhances performance of broiler chickens raised under heat stress conditions. International Journal of Biometeorology60, 1099-1110. doi.org/10.1007/s00484-015-1103-x
Lara, L. J., & Rostagno, M. H. (2013). Impact of heat stress on poultry production. Animals, 3 (2): 356–369. doi.org/10.3390/ani3020356
Mahdavi, A. H., Rahmani, H. R., & Pourreza, J. (2005). Effect of probiotic supplements on egg quality and laying hen’s performance. International Poultry Science Journal4(7), 488-492.
Mashaly, M. M., Hendricks 3rd, G. L., Kalama, M. A., Gehad, A. E., Abbas, A. O., & Patterson, P. H. (2004). Effect of heat stress on production parameters and immune responses of commercial laying hens. Poultry Science83(6), 889-894. doi.org/10.1093/ps/83.6.889
Mikulski, D. 1., Jankowski, J., Naczmanski, J., Mikulska, M., & Demey, V. (2012). Effects of dietary probiotic (Pediococcus acidilactici) supplementation on performance, nutrient digestibility, egg traits, egg yolk cholesterol, and fatty acid profile in laying hens. Poultry Science91(10), 2691-2700. https://doi.org/10.3382/ps.2012-02370
Mohammed, A. A., Jacobs, J. A., Murugesan, G. R., & Cheng, H. W. (2018). Effect of dietary synbiotic supplement on behavioral patterns and growth performance of broiler chickens reared under heat stress. Poultry science, 97(4), 1101-1108. https://doi.org/10.3382/ps/pex421
Mohammed, A. A., Jiang, S., Jacobs, J. A., and Cheng, H. W. (2019). Effect of a synbiotic supplement on cecal microbial ecology, antioxidant status, and immune response of broiler chickens reared under heat stress. Poultry Science, 98(10): 4408-4415. https://doi.org/10.3382/ps/pez246
Mohebbifar, A., Kashani, S., Afsari, M., & Torki, M. (2013). Effects of commercial prebiotic and probiotics of diet on performance of laying hens, egg traits and some blood parameters. Annual Research & Review in Biology, 921-934.
Mookiah, S., Sieo, C. C., Ramasamy, K., Abdullah, N., & Ho, Y. W. (2014). Effects of dietary prebiotics, probiotic and synbiotics on performance, caecal bacterial populations and caecal fermentation concentrations of broiler chickens. Journal of the Science of Food and Agriculture94(2), 341-348. doi.org/10.1002/jsfa.6365
Murugesan, G. R., & Persia, M. E. (2015). Influence of a direct‐fed microbial and xylanase enzyme on the dietary energy uptake efficiency and performance of broiler chickens. Journal of the Science of Food and Agriculture, 95(12), 2521-252. https://doi.org/10.1002/jsfa.6984
Numi, E, Nuotio, L, and Schncitz, C. (1992) The Spring P, Wenk C, Dawson KA and Newman KE (2000) competitive exclusion concept: Development and future. The effects of dietary mannaoligosaccharides on cecal. International Journal of Food Microbiology 15(3-4): 237– 240. doi.org/10.1016/0168-1605(92)90054-7
Pesti, G. M., J. A. Vedenov, J. A. Cason, and L. Billard. (2009). A comparison of methods to estimate nutritional requirements from experimental data. Poultry Science. 50: 16-320. https://doi.org/10.1080/00071660802530639
Radu-Rusu, C. G., Pop, I. M., & Simeanu, D. (2010). Effect of a synbiotic feed additive supplementation on laying hens performance and eggs quality. Lucrări Ştiinţifice, Seria Zootehnie, 53, 89-93.
Renaudeau, D., Collin, A., Yahav, S., De Basilio, V., Gourdine, J. L., & Collier, R. J. (2012). Adaptation to hot climate and strategies to alleviate heat stress in livestock production. Animal6(5), 707-728. doi.org/10.1017/S1751731111002448
Schrezenmeir, J., & de Vrese, M. (2001). Probiotics, prebiotics, and synbiotics—approaching a definition. The American Journal of Clinical Nutrition, 73(2), 361s-364s. doi.org/10.1093/ajcn/73.2.361s
Sejian, V., Bhatta, R., Gaughan, J. B., Dunshea, F. R., andLacetera, N. (2018). Adaptation of animals to heat stress. Animal. 12(s2): s431-s444. doi.org/10.1017/S1751731118001945
Sjofjan, O., Natsir, M.H., Adli, D.N., Adelina, D.D. and Triana, L.M., 2020, March. Effect of symbiotic flour (Lactobacillus sp. and fos) to the egg quality and performance of laying hens. In IOP Conference Series: Earth and Environmental Science (Vol. 465, No. 1, p. 012033).10.1088/1755-1315/465/1/012033.
Sugiharto, S., Yudiarti, T., Isroli, I., Widiastuti, E. and Kusumanti, E., 2017. Dietary supplementation of probiotics in poultry exposed to heat stress–a review. Annals of animal science, 17(3), pp.591-604.
Tang, S. G. H., Sieo, C. C., Ramasamy, K., Saad, W. Z., Wong, H. K., & Ho, Y. W. (2017). Performance, biochemical and haematological responses, and relative organ weights of laying hens fed diets supplemented with prebiotic, probiotic and synbiotic. BMC veterinary research, 13, 1-12. doi.org/ 10.1186/s12917-017-1160-y.
Thompson, K. L., and T. J. Applegate. 2006. Feed withdrawal al- ters small-intestinal morphology and mucus of broilers. Poultry Scienve. 85:1535–1540. doi.org/10.1016/j.vetmic.2011.09.012
Verbrugghe, E., Boyen, F., Gaastra, W., Bekhuis, L., Leyman, B., Van Parys, A., ... & Pasmans, F. (2012). The complex interplay between stress and bacterial infections in animals. Veterinary Microbiology155(2-4), 115-127. doi.org/10.1016/j.vetmic.2011.09.012
Xia, M. S., Hu, C. H., & Xu, Z. R. (2004). Effects of copper-bearing montmorillonite on growth performance, digestive enzyme activities, and intestinal microflora and morphology of male broilers. Poultry Science83(11), 1868-1875.
Yahav, S. (2009). Alleviating heat stress in domestic fowl: different strategies. World’s Poultry Science Journal. 44(32): 719–732. doi.org/10.1017/S004393390900049X
Yu, L. C. H., Wang, J. T., Wei, S. C., & Ni, Y. H. (2012). Host-microbial interactions and regulation of intestinal epithelial barrier function: From physiology to pathology. World journal of Gastrointestinal Pathophysiology3(1), 27.
Yusrizal, C. and T.C. Chen. (2003). Effects of adding chicory fructans in feed on broiler growth    performance, serum cholesterol, and intestinal length. International Journal of Poultry Science, 3: 214-219. doi/full/10.5555/20033119235
Zaboli, G. R., Rahimi, S., Shariatmadari, F., Torshizi, M. A. K., Baghbanzadeh, A., & Mehri, M. (2017). Thermal manipulation during Pre and Post-Hatch on thermotolerance of male broiler chickens exposed to chronic heat stress. Poultry Science96(2), 478-485. doi.org/10.3382/ps/pew344
Zarei, M., Ehsani, M., & Torki, M. (2011). Dietary inclusion of probiotics, prebiotics and synbiotic and evaluating performance of laying hens. American Journal of Agricultural and Biological Sciences6(2), 249-255. doi/full/10.5555/20113280837
Zhan XA, Hu CH, Xu ZR (2003). Effects of fructo-oligosaccharide on growth performance and intestinal microflora and morphology of broiler chicks. Chinese Journal of Veterinary Science. 23(2):196-198. doi/full/10.5555/20033091502
Zhang, C., Zhao, X. H., Yang, L., Chen, X. Y., Jiang, R. S., Jin, S. H., and Geng, Z. Y. (2017). Resveratrol alleviates heat stress-induced impairment of intestinal morphology, microflora, and barrier integrity in broilers. Poultry Science. 96(12): 4325-4332. doi.org/10.3382/ps/pex266
Zhang, Z. F., & Kim, I. H. (2013). Effects of probiotic supplementation in different energy and nutrient density diets on performance, egg quality, excreta microflora, excreta noxious gas emission, and serum cholesterol concentrations in laying hens. Journal of Animal Science91(10), 4781-4787. doi.org/10.2527/jas.2013-6484
Zhu, L., Liao, R., Wu, N., Zhu, G., & Yang, C. (2019). Heat stress mediates changes in fecal microbiome and functional pathways of laying hens. Applied Microbiology and Biotechnology103, 461-472.  doi.org/10.1007/s00253-018-9465-8